Legume Research

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Research Article

Legume Research, volume 45 issue 12 (december 2022) : 1476-1483

Simple Sequence Repeats Marker based Detection of Genetic Diversity of Indian Bean Dolichos lablab (L.) of Family Fabaceae

Vishwajeet Singh, Rajdeep Kudesia, Seema Bhadauria
1Department of Botany, Bundelkhand University, Jhansi-284 128, Uttar Pradesh, India.

  • Submitted24-03-2021|

  • Accepted01-05-2021|

  • First Online 08-06-2021|

  • doi 10.18805/LR-4617

Cite article:- Singh Vishwajeet, Kudesia Rajdeep, Bhadauria Seema (2022). Simple Sequence Repeats Marker based Detection of Genetic Diversity of Indian Bean Dolichos lablab (L.) of Family Fabaceae. Legume Research. 45(12): 1476-1483. doi: 10.18805/LR-4617.

ABSTRACT

Background: Assessment and molecular characterization of genetic diversity among the Dolichos lablab (L.) have huge implication in scheming strategies for breeding. In India, there is less comprehensive information on the potential genetic diversity of lablab bean and this is a major challenge for systematic use of lablab bean in genetic breeding programs. To exploit the available trait of interest, the genetic diversity of the locally available genotypes must be known. The PCR-based SSR approach requires just nanogram amounts of template DNA, subjected to quick detection and less influenced by environment. In this manner SSR have been utilizing broadly to study hereditary assorted qualities of yields.
Methods: In this study, genetic diversity on ten Indian Dolichos lablab (L.) genotypes were surveyed utilizing SSR markers at Department of Botany, Bundelkhand University, Jhansi during 2019-20 got from Indian gene bank. For SSR analysis, 5 primers were used for checking the diversity in Dolichos lablab (L.) and the performance of markers were measured by using 4 parameters (1) PIC, (2) Mean resolving Power, (3) Resolving Power and (4) MI. 
Result: SSR (05) markers produced a total of 51 bands, out of which 45 bands (88.23%) were polymorphic which varies from 6 (Primer-111) to 15 (Primer-AGB-9) with mean of 10.5 bands per primer. The polymorphic information content value ranges from 0.265 to 0.488 with a mean value of 0.390. The value of Matrix index is 3.2. The value of resolving power ranges from 2.2 to 12.60 with a mean value of 7.40. In the UPGMA dendogram, the 10 genotypes were separated into two main clusters with all the primers used. Our present examination uncovered that genetic markers might be effectively used for deciding hereditary variety and connections in Dolichos lablab (L.) genotypes and could be utilized as a part of breeding programs.

REFERENCES

  1. Adebisi, A.A. and Bosch, C.H. (2004). Lablab purpureus (L.) Sweet. In Grubben, G.J.H, Denton O.A., (Eds.): Plant Resources of Tropical Africa (PROTA), no. 2, vegetables. PROTA Foun-dation, Wageningen, The Netherlands / Backhuys, Leiden, The Netherlands / CTA, Wageningen, The Netherlands. 343-348.
  2. Asare, T.A., Gowda, S.B., Galyuon, A.I., Aboagye, L.L., Takrama, F.J. and Timko, P.M. (2010). Assessment of the genetic diversity in cowpea (Vigna unguiculata l. Walp.) germplasm from Ghana using simple sequence repeat markers. Plant Genetic Resources: Characterization and Utilization. 8: 142-150.
  3. Atienza, S.G., Torres, A.M., Millan, T. and Cubero, J.I. (2005). Genetic diversity in Rosa as revealed by RAPDs. Agriculturae Conspectus Scientificus. 70: 75-85.
  4. Beaumont, M.A., Ibrahim, K.M., Boursot, P. and Bruford, M.W. (1998). Measuring genetic distance In: Molecular Tools for Screening and Biodiversity, [Karp, A., Isaac, P. G. and Ingram, D. S. (eds.)] Chapman and Hall, pp.. 315-325. 
  5. Benchimol, L.L., Campos, Tde., Carbonell, S.A.M. and Colombo, C. (2007). Structure of genetic diversity among common bean (Phaseolus vulgaris L.) varieties of Mesoamerican and Andean origins using new developed microsatellite markers. Genetic Resources and Crop Evolution. 54: 1747-1762.
  6. Devos, K.M. and Gale, M.D. (1992). The use of random amplified polymorphic DNA markers in wheat. Theoretical and Applied Genetics. 84: 567-572.
  7. Díaz, M.L. and Buendía, H.F. (2011). Genetic diversity of Colombian land races of common bean as detected through the use of silver-stained and fluorescently labelled microsatellites. Plant Genetic Resources. 9: 86-96.
  8. Doyle, J.J. and Doyle, J.L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin. 19: 119-128.
  9. Gnanesh, B.N., Reddi Sekhar, M., Gopal, K., Reddy, R. and Eswara Reddy, N.P. (2005). Genetic Diversity Analysis of Field bean (Lablab purpureus L. Sweet) through RAPD Markers. Indian Journal of Plant Genetic Resources. 18(3): 233-235.
  10. Gupta, P.K. and Varshney, R.K. (2000). The development and use of microsatellite markers for genetic analysis and plant breeding with emphasis on bread wheat. Euphtyica. 113: 163- 185.
  11. Hair, J.R., Anderson, R.E., Tatham, R.L. and Black, W.C. (1995). Multi- variate data analysis with readings. 4th edition, Prentice-Hall, Englewood Cliffs, NJ.
  12. Keerthi, C.M., Ramesh, S., Byregowda, M. and Vaijayanthi, P.V. (2018). Simple Sequence Repeat (SSR) Marker Assay-Based Genetic Diversity among Dolichos Bean (Lablab purpureus L. Sweet) Advanced Breeding Lines Differing for Productivity per se Traits. International Journal of Current Microbiology and Applied Sciences. 7(5): 3736-3744.
  13. Kernodle, S.P., Cannon, R.E. and Scandalios, J.G. (1993). Concentration of primer and template qualitatively affects product in RAPD-PCR. Biotechniques. 14: 362-364.
  14. Li, G., Ra, W.H., Park, J.W., Kwon, S.W., Lee, J.H., Park, C.B. and Park, Y.J. (2011). Developing EST-SSR Markers to study molecular diversity in Liriope and Ophiopogon. Biochemical Systematics and Ecology. 39: 241-252.
  15. Morgante, M., Hanafey, M. and Powell, W. (2002). Microsatellites are preferentially associated with nonrepetitive DNA in plant genomes. Nature Genetics. 30: 194-200.
  16. Nagy, S., Poczai, P., Cernak, I., Gorgi, A., Hegedus, G. and Taller, J. (2012). PIC calc: An online progrmme to calculate polymorphic information content for molecular genetic studies. Biochemical Genetics. 50: 670-672.
  17. Narshimulu, G., Jamaloddin, M., Vemireddy, L.R., Anuradha, G. and Siddiq, E. (2011). Potentiality of evenly distributed hypervariable microsatellite markers in marker-assisted breeding of rice. Plant Breeding. 130: 314-320.
  18. Patil, P., Venkatesha, S.C., Ashok, T.H., Gowda, T.K.S., Byre and Gowda, M. (2009). Genetic Diversity in Field Bean as Revealed with AFLP Markers. Journal of Food Legumes. 22(1): 18-22.
  19. Peakall, R. and Smouse, P.E. (2006). GENALEX 6.2: genetic analysis in Excel. Population genetic software for teaching and research http://www.anu.edu.au/BoZo/GenAlEx/. Molecular Ecology Notes. 6: 288-295.
  20. Powell, W., Morgante, M. and Andre, C. et al. (1996). The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding. 2: 225-238. 
  21. Prevost, A. and Wilkinson, M.J. (1999). A new system for comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theoretical and Applied Genetics. 98: 107-112.
  22. Rai, N., Singh, P.K., Rai, A.C. and Rai, V.P. (2011). Singh, M. Genetic diversity in Indian bean (Lablab purpureus) germ-plasm based on morphological traits and RAPD markers. Indian Journal of Agricultural Sciences. 81(9): 801-6.
  23. Reif, J.C., Melchinger, A.E., Xia, X.C. and Warburton, M.L. (2003). Genetic distance based on simple sequence repeats and heterosis in tropical maize populations. Crop Science. 43: 1275-1282.
  24. Roldan-Ruiz, I., Dendauw, J., Vanbockstaele, E., Depicker, A. and De Loose, M. (2000). AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.). Molecular Breeding. 6: 125-134.
  25. Singh, S.P. (1999). Improvement of small-seeded race Meso-american cultivars. In Common Bean Improvement in the Twenty-First Century (ed. Singh, S.P.), Kluwer, Dordrecht, 225-274.
  26. Slavov, G.T., Howe, G.T., Gyaourova, A.V., Birkes, D.S. and Adams, W.T. (2005). Estimating pollen flow using SSR markers and paternity exclusion: Accounting for mistyping. Molecular Breeding. 14: 3109-3121.
  27. Sultana, N., Ozaki, Y. and Okubo, H. (2000). The use of RAPD markers in lablab bean (Lablab purpureus (L.) Sweet phylogeny. Bulletin of the Institute of Tropical Agriculture. 23: 45-51.
  28. Tautz, D. (1989). Hypervariability of simple sequences as a general source for polymorphic DNA markers. Nucleic Acids Research. 17(16): 6463-6471.
  29. Tefera, T, Oluoch, M. and Maass, B.L. (2006). Identifying Vegetable Lablab Types by Participatory assessment: Panelists’ Perceptions of Morphological Traits and Organoleptic Taste Assessment. The 13th Australian Society of Agronomy Conference, Perth, 11-14 September.
  30. Tian, Z., Wang, S., Wang, W. and Liu, L. (2005). Study on the Diversity of Germplasm Resources of Dolichos lablab (L.). Natural Science Journal of Hainan University. 23(1): 53-60.
  31. Varshney, R.K., Graner, A. and Sorrells, M.E. (2005). Genic micro-satellite markers in plants: Features and applications. Trends Biotechnol. 23: 48-55.
  32. Varshney, R.K., Chabane, K., Hendre, P.S., Aggarwal, R.K. and Graner, A. (2007). Comparative assessment of EST-SSR, EST-SNP and AFLP markers for evaluation of genetic diversity and conservation of genetic resources using wild, cultivated and elite barleys. Plant Science. 173: 638-649.
  33. Venkatesha, S.C., Gowda, M.B., Mahadevu, P., Rao, A.M., Kim, D.J., Ellis, T.H.N. and Knox, M.R. (2007). Genetic Diversity within Lablab purpureus and the Application of Gene-Specific Markers from a Range of Legume Species. Plant Genetic Resources: Characterization and Utilization. 5(3): 154-171.
  34. Vos, P., Hogers, R., Bleeker, M., Reijans, M., Van de lee, T. and Hornes, M. (1995). AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research. 23: 4407-4414.
  35. Wang, M.L., Gillaspie, A.G., Newman, M.L., Dean, R.E., Pittman, R.N., Morris, J.B. and Pederson, G.A. (2004). Transfer of Simple Sequence Repeat (SSR) Markers across the Legume Family for Germplasm Characterization and Evaluation. Plant Genetic Resources. 2(2): 107-119.
  36. Wang, M.L., Morris, J.B., Barkley, N.A., Dean, R.E., Jenkins, T.M. and Pederson, G.A. (2007). Evaluation of Genetic Diversity of the USDA Lablab purpureus germplasm Col- lection Using Simple Sequence Repeat Markers. Journal of Horticultural Science Biotechnology. 82(4): 571-578.
  37. Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. and Tingey, S.V. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research. 18: 6531-6535.
  38. Woodhead, M., Russell, J., Squirrell, J., Hollingsworth, P.M., Mackenzie, K. and Gibby, M. et al. (2005). Comparative analysis of population genetic structure in Athyrium distentifolium (Pteridophyta) using AFLPs and SSRs from anonymous and transcribed gene regions. Molecular Ecology. 14: 1681-1695.
  39. Zhang, G., Shengchun, Xu., Weihua, M., Yaming, G. and Qizan, Hu. (2013). Development of EST-SSR markers to study genetic diversity in hyacinth bean (Lablab purpureus L.). POJ. 6(4): 295-301.
  40. Zhong, S.B., Yang, B.J. and Alfenas, A.C. (2008).Development of microsatellite markers for the guava rust fungus, Puccinia psidii. Molecular Ecology Notes. 8: 348-350.
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