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Research Article

volume 42 issue 2 (april 2019) : 173-177,   Doi: 10.18805/LR-460

Biochemical responses of sainfoin shoot and root tissues to drought stress in In vitro Culture 

R
Ramazan Beyaz
1Department of Soil Science and Plant Nutrition, Faculty of Agriculture, Kýrþehir Ahi Evran University, Kýrþehir, Turkey.

  • Submitted19-10-2018|

  • Accepted16-12-2018|

  • First Online 23-02-2019|

  • doi 10.18805/LR-460

Cite article:- Beyaz Ramazan (2019). Biochemical responses of sainfoin shoot and root tissues to drought stress in In vitro Culture. Legume Research. 42(2): 173-177. doi: 10.18805/LR-460.

ABSTRACT

This study was conducted to investigate the biochemical responses of the shoot and root tissues of sainfoin to drought stress under in vitro conditions. Seeds of sainfoin were cultured on MS (Murashige and Skoog) medium with addition of concentrations of PEG-6000 (50, 100, and 150 g/l). Biochemical analyzes (CAT, SOD, GR, and APX enzyme activity; proline, malondialdehyde (MDA) and chlorophyll contents) were carried out on the 35-day-old seedlings. The principal results of the study were that CAT and SOD antioxidant enzymes seemed to play a critical role in oxidative stress in both tissues of sainfoin seedlings. On the other hand, a significant decrease in GR activity and no change in APX activity detected in both tissues under stress. The contents of proline and MDA increased in both tissues while the chlorophyll contents decreased in the shoot tissue. Antioxidant enzyme activities seemed to be more active in the root tissue than the shoot tissue. Accumulation of proline was higher in the root tissue, while the MDA content was higher in the shoot tissue of the seedlings. 

REFERENCES

  1. Almeselmani, M., Deshmukh, P.S., Sairam, R.K., Kushwaha, S.R., Singh, T.P. (2006). Protective role of antioxidant enzymes under high temperature stress. Plant Science, 171:382-388.
  2. Bates, L.S., Waldren, R.P., Teare, I.D. (1973). Rapid determination of free proline for water stress studies. Plant Soil, 39:205-207.
  3. Beyaz, R., Sancak, C., Yildiz, M. (2017). Morphological and biochemical responses of sainfoin (Onobrychis viciifolia Scop.) ecotypes to salinity. Legume Research, 41(2): 253-258.
  4. Bhattarai, S. (2017). Characterization of diverse germplasm of sainfoin (Onobrychis viciifolia Scop.) using agro-morphological traits and AFLP molecular markers, Thesis (M.S.), University of Saskatchewan.
  5. Cakmak, I. and Marschner, H. (1992). Magnesium defficiency and higlight intensity enhance activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase in bean leaves. Plant Physiol, 98:1222-1226.
  6. Carbonero, C.H., Mueller-Harvey, I., Brown, T.A., Smith, L. (2011). Sainfoin (Onobrychis viciifolia): A beneficial forage legume. Plant Genet. Resour.; Characterization Util, 9:70-85. 
  7. Curtis, O.F. and Shetty, K. (1996). Growth medium effects on vitrification, total phenolics, chlorophyll, and water content of in vitro propagated oregano clones. Acta Horticulture 426: 498-503.
  8. Çakmak, I., Atlý, M., Kaya, R., Evliya, H., Marschner, H. (1994). Association of high light and zinc deficiency in cold-induced leaf chlorosis in grapefruit and mandarin trees. J. Plant Physiol, 146: 355-360.
  9. Guo, Y.Y., Yua, H.Y., Yang, M.M., Kong, D.S., Zhang, Y.J. (2018). Effect of drought stress on lipid peroxidation, osmotic adjustment and antioxidant enzyme activity of leaves and roots of Lycium ruthenicum Murr. seedling. Russ. J. Plant Physiol, 65(2): 244-250.
  10. Hayat, S., Hayat, Q., Alyemeni, M.N., Wani, A.S., Pichtel, J., Ahmad, A. (2012). Role of proline under changing environments. Plant Signaling & Behavior, 7(11): 1456-1466.
  11. Hosseini, S.M. and Hasanloo, S.M. (2015). Physiological characteristics, antioxidant enzyme activities, and gene expression in spring canola (Brassica napus L.) cultivars under drought stress conditions. Turkish Journal of Botany, 39:413-420.
  12. Irani, S., Majidi, M.M., Mirlohi, A., Zargar, M., Karami, K. (2015). Assessment of drought tolerance in sainfoin: physiological and drought tolerance ýndices. Agronomy Journal, 107(5): 1771-1781.
  13. Kavas, M., Baloðlu, M.C., Akça, O., Köse, F.S., Gökçay, D. (2013). Effect of drought stress on oxidative damage and antioxidant enzyme activity in melon seedlings. Turkish Journal of Biology, 37: 491-498.
  14. Kölliker, R., Kempf, K., Malisch, C.S., Lüscher, A. (2017). Promising options for improving performance and proanthocyanidins of the forage legume sainfoin (Onobrychis viciifolia Scop.). Euphytica, 213:179.
  15. Lutts, S., Kine, J.M., Bouharmont, J. (1996). NaCl-Induced Senesence in leaves of rice (oryza sativa l.) cultivars differing in salinity resistance. Annual Botany, 78: 389-398.
  16. Meher, Shivakrishna, P., Reddy, K.A., Rao, D.M. (2018). Effect of PEG-6000 imposed drought stress on RNA content, relative water content (RWC), and chlorophyll content in peanut leaves and roots. Saudi Journal of Biology Sciences, 25: 285-289.
  17. Mohajer, S., Taha, R.M., Mohajer, M., Esmaeili, A.K. (2014). Micropropagation of Bioencapsulation and Ultrastructural Features of Sainfoin (Onobrychis viciifolia) Grown In Vivo and In Vitro. The Scientific World Journal, 2014: 1-12.
  18. Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15:473-497.
  19. Özaslan-Parlak, A. and Parlak, M. (2008). Effect of salinity in irrigation water on some plant development parameters of sainfoin (Onobrychis viciifolia Scop.) and soil salinity. Journal of Agricultural Science, 14: 320-325.
  20. Piwowarczyk, B., Kamiñska, I., Rybiñski, W. (2014). Influence of PEG generated osmotic stress on shoot regeneration and some biochemical parameters in lathyrus culture. Czech Journal of Genetics Plant and Plant Breeding, 50 (2): 77-83.
  21. Puangbuta, D., Jogloya, S., Vorasoota, N. (2017). Association of photosynthetic traits with water use efficiency andSPAD chlorophyll meter reading of Jerusalem artichoke under droughtconditions. Agricultural Water Managen, 188: 29-35.
  22. Ren, J., Sun, L.N., Zhang, Q.Y., Song, X.S. (2016). Drought tolerance is correlated with the activity of antioxidant enzymes in Cerasus humilis Seedlings. BioMed Research International, :1-9.
  23. Sancak, C. (1999). In Vitro micropropagation of sainfoin (Onobrychis viciifolia Scop.). Turkish Journal of Biology, 23 (1999):133-136.
  24. ªen, A. (2012). Oxidative stress studies in plant tissue culture, antioxidant enzyme, In: Intech book. [El-Missiry, M.A., (Eds.)] E-    Publishing Inc, pp. 60-69.
  25. Talbi, S., Romero-Puertas, M.C., Hernández, A., Terrón, L., Ferchichi, A., Sandalio, L.M. (2015). Drought tolerance in a Saharian plant Oudneya africana: Role of antioxidant defences. Environmental and Experimantal Botany, 111 (2015):114-126.
  26. Tambussi, E.A., Bartoli, C.G., Bettran, J., Guiamet, J.J., Araus, J.C. (2000). Oxidative damage to thylakoids proteins in water stressed leaves of wheat (Triticum aestivum L). Physiologia Plantarum, 108:398-404.
  27. Uzun, S., Avcý, S., Ozcan, S., Sancak, C. (2017). Effects of NaCl on germination and seedling characteristics of different onobrychis taxa. Fresenius Environmental Bulletin, 26(11): 6317-6323. 
  28. Zhang, L., Peng, J., Chen, T., Zhao, X.H., Zhang, S.P., Liu, S.D., Dong, H.L., Feng, L.L., Yu, H.X. (2014). Effect of Drought stress on lipid peroxidation and proline content in cotton roots. The Journal of Animal & Plant Sciences, 24(6):1729-1736. 
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    Research Article

    volume 42 issue 2 (april 2019) : 173-177,   Doi: 10.18805/LR-460

    Biochemical responses of sainfoin shoot and root tissues to drought stress in In vitro Culture 

    R
    Ramazan Beyaz
    1Department of Soil Science and Plant Nutrition, Faculty of Agriculture, Kýrþehir Ahi Evran University, Kýrþehir, Turkey.

    • Submitted19-10-2018|

    • Accepted16-12-2018|

    • First Online 23-02-2019|

    • doi 10.18805/LR-460

    Cite article:- Beyaz Ramazan (2019). Biochemical responses of sainfoin shoot and root tissues to drought stress in In vitro Culture. Legume Research. 42(2): 173-177. doi: 10.18805/LR-460.

    ABSTRACT

    This study was conducted to investigate the biochemical responses of the shoot and root tissues of sainfoin to drought stress under in vitro conditions. Seeds of sainfoin were cultured on MS (Murashige and Skoog) medium with addition of concentrations of PEG-6000 (50, 100, and 150 g/l). Biochemical analyzes (CAT, SOD, GR, and APX enzyme activity; proline, malondialdehyde (MDA) and chlorophyll contents) were carried out on the 35-day-old seedlings. The principal results of the study were that CAT and SOD antioxidant enzymes seemed to play a critical role in oxidative stress in both tissues of sainfoin seedlings. On the other hand, a significant decrease in GR activity and no change in APX activity detected in both tissues under stress. The contents of proline and MDA increased in both tissues while the chlorophyll contents decreased in the shoot tissue. Antioxidant enzyme activities seemed to be more active in the root tissue than the shoot tissue. Accumulation of proline was higher in the root tissue, while the MDA content was higher in the shoot tissue of the seedlings. 

    REFERENCES

    1. Almeselmani, M., Deshmukh, P.S., Sairam, R.K., Kushwaha, S.R., Singh, T.P. (2006). Protective role of antioxidant enzymes under high temperature stress. Plant Science, 171:382-388.
    2. Bates, L.S., Waldren, R.P., Teare, I.D. (1973). Rapid determination of free proline for water stress studies. Plant Soil, 39:205-207.
    3. Beyaz, R., Sancak, C., Yildiz, M. (2017). Morphological and biochemical responses of sainfoin (Onobrychis viciifolia Scop.) ecotypes to salinity. Legume Research, 41(2): 253-258.
    4. Bhattarai, S. (2017). Characterization of diverse germplasm of sainfoin (Onobrychis viciifolia Scop.) using agro-morphological traits and AFLP molecular markers, Thesis (M.S.), University of Saskatchewan.
    5. Cakmak, I. and Marschner, H. (1992). Magnesium defficiency and higlight intensity enhance activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase in bean leaves. Plant Physiol, 98:1222-1226.
    6. Carbonero, C.H., Mueller-Harvey, I., Brown, T.A., Smith, L. (2011). Sainfoin (Onobrychis viciifolia): A beneficial forage legume. Plant Genet. Resour.; Characterization Util, 9:70-85. 
    7. Curtis, O.F. and Shetty, K. (1996). Growth medium effects on vitrification, total phenolics, chlorophyll, and water content of in vitro propagated oregano clones. Acta Horticulture 426: 498-503.
    8. Çakmak, I., Atlý, M., Kaya, R., Evliya, H., Marschner, H. (1994). Association of high light and zinc deficiency in cold-induced leaf chlorosis in grapefruit and mandarin trees. J. Plant Physiol, 146: 355-360.
    9. Guo, Y.Y., Yua, H.Y., Yang, M.M., Kong, D.S., Zhang, Y.J. (2018). Effect of drought stress on lipid peroxidation, osmotic adjustment and antioxidant enzyme activity of leaves and roots of Lycium ruthenicum Murr. seedling. Russ. J. Plant Physiol, 65(2): 244-250.
    10. Hayat, S., Hayat, Q., Alyemeni, M.N., Wani, A.S., Pichtel, J., Ahmad, A. (2012). Role of proline under changing environments. Plant Signaling & Behavior, 7(11): 1456-1466.
    11. Hosseini, S.M. and Hasanloo, S.M. (2015). Physiological characteristics, antioxidant enzyme activities, and gene expression in spring canola (Brassica napus L.) cultivars under drought stress conditions. Turkish Journal of Botany, 39:413-420.
    12. Irani, S., Majidi, M.M., Mirlohi, A., Zargar, M., Karami, K. (2015). Assessment of drought tolerance in sainfoin: physiological and drought tolerance ýndices. Agronomy Journal, 107(5): 1771-1781.
    13. Kavas, M., Baloðlu, M.C., Akça, O., Köse, F.S., Gökçay, D. (2013). Effect of drought stress on oxidative damage and antioxidant enzyme activity in melon seedlings. Turkish Journal of Biology, 37: 491-498.
    14. Kölliker, R., Kempf, K., Malisch, C.S., Lüscher, A. (2017). Promising options for improving performance and proanthocyanidins of the forage legume sainfoin (Onobrychis viciifolia Scop.). Euphytica, 213:179.
    15. Lutts, S., Kine, J.M., Bouharmont, J. (1996). NaCl-Induced Senesence in leaves of rice (oryza sativa l.) cultivars differing in salinity resistance. Annual Botany, 78: 389-398.
    16. Meher, Shivakrishna, P., Reddy, K.A., Rao, D.M. (2018). Effect of PEG-6000 imposed drought stress on RNA content, relative water content (RWC), and chlorophyll content in peanut leaves and roots. Saudi Journal of Biology Sciences, 25: 285-289.
    17. Mohajer, S., Taha, R.M., Mohajer, M., Esmaeili, A.K. (2014). Micropropagation of Bioencapsulation and Ultrastructural Features of Sainfoin (Onobrychis viciifolia) Grown In Vivo and In Vitro. The Scientific World Journal, 2014: 1-12.
    18. Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15:473-497.
    19. Özaslan-Parlak, A. and Parlak, M. (2008). Effect of salinity in irrigation water on some plant development parameters of sainfoin (Onobrychis viciifolia Scop.) and soil salinity. Journal of Agricultural Science, 14: 320-325.
    20. Piwowarczyk, B., Kamiñska, I., Rybiñski, W. (2014). Influence of PEG generated osmotic stress on shoot regeneration and some biochemical parameters in lathyrus culture. Czech Journal of Genetics Plant and Plant Breeding, 50 (2): 77-83.
    21. Puangbuta, D., Jogloya, S., Vorasoota, N. (2017). Association of photosynthetic traits with water use efficiency andSPAD chlorophyll meter reading of Jerusalem artichoke under droughtconditions. Agricultural Water Managen, 188: 29-35.
    22. Ren, J., Sun, L.N., Zhang, Q.Y., Song, X.S. (2016). Drought tolerance is correlated with the activity of antioxidant enzymes in Cerasus humilis Seedlings. BioMed Research International, :1-9.
    23. Sancak, C. (1999). In Vitro micropropagation of sainfoin (Onobrychis viciifolia Scop.). Turkish Journal of Biology, 23 (1999):133-136.
    24. ªen, A. (2012). Oxidative stress studies in plant tissue culture, antioxidant enzyme, In: Intech book. [El-Missiry, M.A., (Eds.)] E-    Publishing Inc, pp. 60-69.
    25. Talbi, S., Romero-Puertas, M.C., Hernández, A., Terrón, L., Ferchichi, A., Sandalio, L.M. (2015). Drought tolerance in a Saharian plant Oudneya africana: Role of antioxidant defences. Environmental and Experimantal Botany, 111 (2015):114-126.
    26. Tambussi, E.A., Bartoli, C.G., Bettran, J., Guiamet, J.J., Araus, J.C. (2000). Oxidative damage to thylakoids proteins in water stressed leaves of wheat (Triticum aestivum L). Physiologia Plantarum, 108:398-404.
    27. Uzun, S., Avcý, S., Ozcan, S., Sancak, C. (2017). Effects of NaCl on germination and seedling characteristics of different onobrychis taxa. Fresenius Environmental Bulletin, 26(11): 6317-6323. 
    28. Zhang, L., Peng, J., Chen, T., Zhao, X.H., Zhang, S.P., Liu, S.D., Dong, H.L., Feng, L.L., Yu, H.X. (2014). Effect of Drought stress on lipid peroxidation and proline content in cotton roots. The Journal of Animal & Plant Sciences, 24(6):1729-1736. 
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