DNA Fingerprinting of Vegetable Soybean Cultivar ‘Zhexian No.9’ Using 101 New Developed HRM-Based SNP Markers

DOI: 10.18805/LR-456    | Article Id: LR-456 | Page : 8-17
Citation :- DNA Fingerprinting of Vegetable Soybean Cultivar ‘Zhexian No.9’ Using 101 New Developed HRM-Based SNP Markers.Legume Research-An International Journal.2020.(43):8-17
X.J. Fu, J.X. Pei, Y.T. Zheng, D.D. Guo, Q.H. Yang, H.X. Jin, D.H. Zhu, D.K. Dong and S.C. Xu shengchun2001@163.com
Address : Hangzhou National Sub-center of Soybean Improvement, Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences. Hangzhou, Zhejiang 310021, P.R. China.
Submitted Date : 10-10-2018
Accepted Date : 29-06-2019

Abstract

Single nucleotide polymorphisms (SNPs) have been proved to be powerful markers in genetic analysis due to their high abundance and polymorphism in plant genomes. The recently developed high-resolution melting (HRM) analysis method provides a novel, quick, and close-tube PCR approach to analyze SNP variations. In present study, 101 HRM-based SNP markers from 20 soybean chromosomes were developed for genotyping vegetable soybean cultivar ‘Zhexian No.9’ with ‘Williams 82’ as reference. 33.7% of these markers were polymorphic between ‘Zhexian No.9’ and ‘Williams 82’. Polymorphic markers were found on 85% (17 of 20) of the soybean chromosomes when comparing ‘Zhexian No.9’ and ‘Williams 82’. Finally, an array of 101 in-sequence nucleotide letters was generated as the first precise SNP fingerprint of ‘Zhexian No.9’. The described marker-developing methodology could be used in other crops with known genomic information. 

Keywords

HRM SNP fingerprint Soybean ‘Zhexian No.9’

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