Analysis of the effects of coumarin on Lens culinaris Medik by some biochemical parameters using real-time polymerase chain reaction

DOI: 10.18805/LR-439    | Article Id: LR-439 | Page : 640-645
Citation :- Analysis of the effects of coumarin on Lens culinaris Medik by some biochemical parameters using real-time polymerase chain reaction.Legume Research-An International Journal.2019.(42):640-645
Burcu Yuksel and Ozlem Aksoy burcu.yuksel@yahoo.com
Address : Kocaeli University, Vocational School of Kocaeli Health Sciences Kocaeli, 41380, Turkey.
Submitted Date : 1-07-2018
Accepted Date : 21-12-2018

Abstract

This study aimed to reveal the effects of coumarin on Lens culinaris Medik. The germinated and ungerminated seed counts of the experimental groups were determined and the EC50 value was calculated by probit analysis. In biochemical studies, catalase and superoxide dismutase enzyme activities were investigated together with lipid peroxidation and hydrogen peroxide quantities. The results demonstrated an increase in the amount of malondialdehyde, a measure of lipid peroxidation, a decrease in the amount of H2O2 and EC50 value in the CAT activity and an increase in the EC50 x 2 value. In real-time PCR analysis, three different genetic expressions related to abiotic stress (CAT, Cu / Zn SOD and Mn SOD gene expression) were examined. It was determined that coumarin caused genotoxic and biochemical damage on L. culinaris. 

Keywords

Catalase L. culinaris Coumarin RT PCR Superoxide dismutase.

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