Phenotypic characterization of indigenous rhizobia nodulating chickpea in Turkey reveals high diversity
 

DOI: 10.18805/LR-430    | Article Id: LR-430 | Page : 379-384
Citation :- Phenotypic characterization of indigenous rhizobia nodulating chickpea in Turkey reveals high diversity.Legume Research-An International Journal.2019.(42):379-384
M.A. Mahmoud, D. Mart and C. Can ayselcan1938@gmail.com
Address : Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt.
Submitted Date : 16-05-2018
Accepted Date : 21-08-2018

Abstract

A total of 120 root-nodule bacteria from chickpea plants (Cicer arietinum L.) from six regions in Turkey were characterized using 71 phenotypes. Utilization of carbon and nitrogen sources, tolerance to salt stress, temperature and pH, resistance to antibiotics and heavy metals and ability to produce some enzymes, were assessed. 90% of the isolates produced mucously, circular, smooth-margined and watery to creamy colonies with 2–4 mm diameter after 1-3 days of growth. The isolates utilized different compounds as sole carbon and nitrogen sources, endured 2% salt, grew optimally at 25-35°C and pHs between 6-8, exhibited insensitivity to the heavy metals Zn, Hg, and Cu and the antibiotics kanamycin, streptomycin and tetracycline. Numerical analysis separated the isolates into three clusters at 25% similarity. Results revealed diversity among isolates and were consistent with previous findings on chickpea Mesorhizobia.      

Keywords

Chickpea Diversity Phenotypic characterization Rhizobia.

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