Legume Research

  • Chief EditorJ. S. Sandhu

  • Print ISSN 0250-5371

  • Online ISSN 0976-0571

  • NAAS Rating 6.59

  • SJR .349 (2022) Q2

  • Impact Factor .669 (2022)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus

Genetic Diversity Assessment in Chickpea (Cicer arietinum L.) through Agro-morphological and ISSR Molecular Markers

D.K. Janghel, Krishan Kumar, Mukesh Kumar, A.K. Chhabra
  • Email
1Department of Genetics and Plant Breeding, College of Agriculture, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125 004, Haryana, India.
  • Submitted03-12-2019|

  • Accepted30-04-2020|

  • First Online 28-07-2020|

  • doi 10.18805/LR-4293

Cite article:- Janghel D.K., Kumar Krishan, Kumar Mukesh, Chhabra A.K. (2021). Genetic Diversity Assessment in Chickpea (Cicer arietinum L.) through Agro-morphological and ISSR Molecular Markers. Legume Research. 44(7): 751-758. doi: 10.18805/LR-4293.
Background: Chickpea (Cicer arietinum L.), commonly known as gram or Bengal gram, is a self-pollinated diploid plant (2n= 2x= 16) ranked third after common bean and field pea. Genetic diversity assessment is the fundamental of any breeding programme, conservation of genetic resources and as a general guide for the choice of parents in hybrid breeding. The present investigation aimed to study the genetic diversity among 45 elite chickpea genotypes using agro-morphological traits and ISSR molecular markers.
Methods: The experimental material comprised of 45 elite chickpea genotypes, grown in Randomized Block Design (RBD) with three replications under natural field conditions at Research Farm of Pulses Section, CCSHAU, Hisar during Rabi 2014-15. Genetic divergence was studied using 11 agro-morphological traits by Non-hierarchical Euclidean cluster analysis and 25 ISSR primers using UPGMA based method.
Result: Genetic divergence study based on agro-morphological traits and molecular markers showed ample amount of genetic variation among elite chickpea genotypes which were discussed and compared their diversity analysis as well. The present study confirmed the importance of agro-morphological traits and ISSR markers for detecting tremendous amount of genetic diversity in chickpea which may be used to select good parental material in chickpea breeding programmes for further improvement.
  1. Aggarwal, H., Rao, A., Rana, J.S., Singh, J., Kumar, A., Chhokar, V., Beniwal, V. (2015). Assessment of genetic diversity among 125 cultivars of chickpea (Cicer arietinum L.) of Indian origin using ISSR markers. Turkish Journal of Botany. 39: 218-226.
  2. Aggarwal, H., Rao, A., Rana, J.S., Singh, J., Kumar, A., Chhokar, V., Beniwal, V. (2011). Inter-simple sequence repeats reveal significant genetic diversity among chickpea (Cicer arietinum L.) cultivars. Journal of Plant Science. 6: 202-212.
  3. Babayeva, S.M., Nasibova, J.A., Akparov, Z.I., Shikhaliyeva, K.B., Mammadova, A.D., Izzatullayeva, V.I., Abbasov, M.A. (2018). Application of DNA markers in determination of fusarium resistance and genetic diversity in chickpea. Legume Research. 41(4): 537-542.
  4. Burton, G. and Devane, E.M. (1953). Estimating heritability in tall festuca (Festuca arundinacea) from replicated clonal material. Agronomy Journals. 45: 478-481.
  5. FAO (2013). FAOSTAT. Food and Agriculture Organization of the United Nations. http://faostat.fao.org/default.aspx.
  6. Hanson, C.H., Robinson, H.F., Comstock, R.E. (1956). Biometrical studies in yield in segregating populations of Korean lespediza. Agronomy Journal. 48: 214-318.
  7. Johnson, H.W., Robinson, H.F., Comstock, R.E. (1955). Estimates of genetic and environmental variability in soybean. Agronomy Journal. 47: 314-318.
  8. Johnson, P.L., Sai, S., Nanda, H.C., Sharma, R.N. (2015). Variability and stability analysis for seed yield and its components in chickpea (Cicer arietinum L.). Journal of Food Science and Agricultural Technology. 1(1): 152-156.
  9. Mallu, T.S., Mwangi, S.G., Nyende, A.B., Rao, N.V.P.R.G., Odeny, D.A., Rathore, A., Kumar, A. (2015). Assessment of genetic variation and heritability of agronomic traits in chickpea (Cicer arietinum L.). International Journal of Agronomy and Agricultural Research. 6(1): 77-88.
  10. Nizama, J.R., Patel, S.R., Patel, A.I. (2013). Genetic variability and heritability among quantitative traits in chickpea under tropical region. Asian Resonance. 2(4): 45-48.
  11. Nkongolo, K.K., Michael, P., Gratton, W.S. (2002). Cloning and characterization of RAPD markers inferring genetic relationships among pine species. Genome. 45: 51-58.
  12. Ojha, V.S., Shiva, N., Singh, R. (2010). Genetic variability in chickpea (Cicer arietinum L.). Progressive Research. 5(2): 275-276.
  13. Peerzada, O.H., Chaurasia, A.K., Ahmad, N.I. (2014). Evaluation of chickpea germplasm (Cicer arietinum L.) Desi for yield and yield contributing traits. An International Quarterly Journal of Life Sciences. 9(4): 1805-1809.
  14. Rasool, S. (2013). Genetic diversity as revealed by RAPD analysis among chickpea genotypes. Pakistan Journal of Botany. 45(3): 829-834.
  15. Rohlf, R.J. (1990). NTSYS-PC, numerical taxonomy and Multivariate Analysis System, Version 1.8. Exeter Software, Setauket, New York. http://www.exetersoftware.com/cat/ntsyspc/    ntsyspc.htm.
  16. Saghai-Maroof, M.A., Solima, K.M., Jorgenson, R.A., Allard, R.W. (1984). Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location and population dynamics. Proceedings of the Natural Academy of Sciences. USA, 81: 8014-8018.
  17. Singh, P.K., Sharma, H., Srivastava, N., Bhagyawant, S.S. (2014). Analysis of genetic diversity among wild and cultivated chickpea genotypes employing ISSR and RAPD markers. American Journal of Plant Sciences. 5: 676-682.
  18. Spark, D.N. (1973). Euclidean cluster analysis. Algorithm As. 58. Applied Statistics. 22: 126-130.
  19. Sreelakshmi, C., Shivani, D., Kumar, C.V.S. (2010). Genetic divergence, variability and character association studies in Bengal gram (Cicer arietinum L.). Electronic Journal of Plant Breeding. 1(5): 1339-1343.
  20. Sudupak, A., Akkaya, S., Kence, A. (2002). Analysis of genetic relationships among perennial and annual Cicer species growing in Turkey using RAPD markers. Theoretical and Applied Genetics. 105: 1220-1228.
  21. Varshney, R.K., Song, C., Saxena, R.K., Azam, S., Yu, S., Sharpe, A.G., Cannon, S., Baek, J., Rosen, B.D., Tar’an B. (2013). Draft genome sequence of chickpea (Cicer arietinum) provides a resource for trait improvement. Nature Biotechnology. 31: 240-248.
  22. Wang, N., Hatcher, D.W., Tyler, R.T., Toews, R., Gawalko, E.J. (2010). Effect of cooking on the Composition of beans (Phaseolus vulgaris L.) and chickpea (Cicer arietinum L.). Food Research International. 43: 589-594.

Editorial Board

View all (0)