Legume Research

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Research Article

Legume Research, volume 45 issue 12 (december 2022) : 1506-1516

Differential Responses on Chlorophyll Content, Osmolyte Accumulation and Membrane Damage Parameters under Salinity Stress on Tolerant and Susceptible Genotypes of Groundnut

Apurba Pal, Anjan Kumar Pal
1College of Horticulture, Khuntpani, Birsa Agricultural University, Kanke, Ranchi-834 006 Jharkhand, India. 

  • Submitted18-11-2019|

  • Accepted24-02-2020|

  • First Online 18-06-2020|

  • doi 10.18805/LR-4284

Cite article:- Pal Apurba, Pal Kumar Anjan (2022). Differential Responses on Chlorophyll Content, Osmolyte Accumulation and Membrane Damage Parameters under Salinity Stress on Tolerant and Susceptible Genotypes of Groundnut. Legume Research. 45(12): 1506-1516. doi: 10.18805/LR-4284.

ABSTRACT

Salinity can affect different physiological activity of plant in various ways. A controlled study was conducted to screen 26 genotypes of groundnut under 200mM NaCl salinity stress. The salt tolerance index or STI of the genotypes ranged from 47.57% to 96.40%. Out of all the genotypes KDG-197 (STI= 96.40%) was found to be the most tolerant under a salinity stress of 200 mM NaCl and it was closely followed by R 2001-2 (STI=87.92%), VG 315 (STI=84.05%), TCGS 1157 (STI=77.59%) and TG 51 (STI=73.67%). While the genotypes Girnar 3 (STI= 47.57%), OG 52-1 (STI=49.09%), TVG 0856 (STI= 49.28%) and J 86 (STI= 50.66%) were the most susceptible genotypes based on their relative performance under stress in respect of total dry weight. It has been noted further that  out of the nine genotypes, KDG 197 registered the minimum reduction (4.51% over control, 2.70% over control) in total chlorophyll and sugar accumulation respectively under NaCl stress whereas, Girnar 3 recorded the highest reduction in both parameters (60.00%, 70.32% over control) respectively, under saline condition. The genotype KDG 197 and R 2001-2 accounted for the highest increase in soluble protein and proline content in their leaves (144.02%, 780.16% over control) respectively than Girnar 3. KDG 197 recorded the minimum (3.39%) increase in lipid peroxidation under stress followed by R 2001-2 with an increase of 13.04% over control plants. In contrast, Girnar 3 registered the highest increase of TBARS content and electrolyte leakage (44.44%, 31.47% over control respectively) indicating maximum membrane damage but R 2001-2 recorded the minimum (3.00%) increase in electrolyte leakage percentage than Girnar 3 (31.47% over control) followed by OG 52-1 (26.14% over control) under stress. So, better osmotic adjustment through accumulation of proline, less membrane damage the leaves helped the tolerant genotypes to sustain under salinity stress in a better way than the susceptible genotypes. 

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