Legume Research

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Legume Research, volume 45 issue 12 (december 2022) : 1484-1489

Crossability Barriers in Interspecific Hybridization of Ricebean [Vigna umbellata (Thunb.) Ohwi and Ohashi] with Other Vigna Species

Neelam Bhardwaj, Tanuja Kapoor, Parveen Sharma
1Rice and Wheat Research Centre, Malan-176 047, Himachal Pradesh, India.
  • Submitted05-11-2019|

  • Accepted01-09-2020|

  • First Online 12-03-2021|

  • doi 10.18805/LR-4277

Cite article:- Bhardwaj Neelam, Kapoor Tanuja, Sharma Parveen (2022). Crossability Barriers in Interspecific Hybridization of Ricebean [Vigna umbellata (Thunb.) Ohwi and Ohashi] with Other Vigna Species. Legume Research. 45(12): 1484-1489. doi: 10.18805/LR-4277.
Background: Ricebean [Vigna umbellata (Thunb.) Ohwi and Ohashi] is a multipurpose grain legume of Mid-Himalayan region mainly cultivated for food, fodder, green manure and has emerged as a good alternative to other pulse crops such as blackgram and greengram which do not flourish in this region due to their susceptibility to cold temperature stress. It is well reported that the nutritional value of ricebean is higher as compared to many other legumes of the Vigna family and has some superior qualities greater than greengram, blackgram and cowpea. It is also resistance to drought, diseases and pests specially the storage pests during growth period and possesses high percentage of seed viability. Despite having all the favourable traits, it is not much popular among the farmers due to the late maturity and indeterminate growth habit. Instead, farmers prefer other crops which fit easily into their cropping pattern and are easy to harvest. A little genetic improvement with respect to maturity and growth habit can revive its cultivation and show great results in its production as a valuable crop. Thus, the present investigation was formulated to introgress desired traits from mash and adzukibean into otherwise high yielding ricebean genotypes using inter-specific hybridization.  
Methods: The present investigation involves the inter-specific hybridization among three Vigna species viz, ricebean (Vigna umbellata), blackgram (Vigna mungo) and adzukibean (Vigna angularis). In the year 2017, six genotypes of ricebean (RBHP-36, RBHP-38, RBHP-43, RBHP-61, RBHP-107 and RBHP-108) were crossed with two genotypes of blackgram (HimMash-1 and Palampur-93) and one genotype of adzukibean (HPU-51) in glasshouse conditions. 
Result: The study revealed that successful crosses were possible only between ricebean and blackgram. All the Inter-specific crosses showed very low pod set percentage ranging from 0 -4% and F1 germination percentage ranging from 20-42%. Pod set percentage and pods harvested varied with combinations of two parental cultivars of each species for most of the inter-specific hybrids. The successful pod set was observed in 16 out of 36 inter-specific crosses. Highest crossability was observed in blackgram and ricebean crosses. Crossing of adzukibean with ricebean showed poor or no pod set among the entire cross combinations which are attributed to early embryo abortion and degeneration during embryogenesis.
  1. Ahn, C.S and Hartmann, R.W. (1977). Interspecific hybridization among four species of the genus Vigna. Proceedings for the First International Mungbean Symposium. Asian vegetable Research and Development Centre. Shanhua. Taiwan: 240-246.
  2. Bharathi, A, Selvaraj, K.S.V., Veerabadhiran, P. and Laksh, B.S. (2006). Crossability barriers in mungbean [Vignaradiata (L.) Wilczek]: with its wild relatives. Indian J. of Crop Sci. 1: 120-124.
  3. Chaisan T., Somta, P., Srinives, P., Chanprame,S., Kaveeta, R. and Dumrongkittikule, S. (2013). Development of tetraploid plants from an inter-specific hybrid between mungbean (Vigna radiata) and ricebean (Vigna umbellata). J. Crop Sci. Biotechnology. 16: 45-51.
  4. Honglin C., Xin, C., Jing, T., Yong, Y., Zhenxing, L., Xiyu, H., Lixia, W., Suhua, W., Jie, L., Liya, Z., Fengxiang, Y. and Xuzhen, C. (2016). Development of gene-based SSR markers in rice bean (Vigna umbellata L.) based on Transcriptome Data. PLoS One. 11(3): e0151040.
  5. Chowdhury, R.K. and Chowdhury, J.B. (1977). Inter generic hybridization between Vigna mungo L. Hepper and Phaseolus calcaratus RoxB. Indian J. Agric. Sci. 47: 117-121.
  6. Dhiman, R., Mittal, R.K., Chaudhary H.K., Yadav, A.K. (2013). Crossability relationship between blackgram (Vigna mungo) and ricebean (V. umbellata) for successful blackgram x ricebean hybridization programme. Indian J. of Agric. Sci. 83: 907-911.
  7. Gopinathan, M.C., Babu, C.R. and Shivanna, K.R. (1986). Interspecific hybridization between rice bean (Vigna umbellata) and its wild relative (V. minima): fertility sterility relationships. Euphytica. 35: 1017-1022.
  8. Nandini, C., Savitharamma, D., Pushpa Doddaraju, L. and Kumar, P. (2020). Polymorphic SSR marker identification for water use efficiency in groundnut (Arachis hypogea L.) parental lines. Legume Res.-An International. J. 43: 303-311.
  9. Nishant Bhanu, A., Singh, M.N. and Srivastava, K. (2018). Crossability studies of interspecific hybridization among Vigna species. Biomed J. Sci Tech Res. DOI: 10.26717/BJSTR.2018. 02.000818.
  10. Prince Lekhi, Gill, R.K. and Bains, T.S. (2018). Generation of interspecific hybrids for introgression of mungbean yellow mosaic virus resistance in Vigna radiata (L.) Wilczek. Legume Res.-An International Journal. 41(4): 526-531.
  11. Rashid, K., Daran, A.B.M., Nezhadahmadi, A., Yusof, M.F.B.M., Azhar, S., Efzueni, S. (2013). Intra-specific crosses and morphological studies of two cultivars of Vigna radiata through in-vitro and in-vivo techniques. Life Sci. J. 10: 2549-2555.
  12. Shayla, (2016). Introgression of urdbean anthracnose resistance and yield components involving Vigna mungo x V. umbellate hybridization. Ph.D. Thesis, Department of Crop Improvement, CSKHPKV, Palampur.
  13. Stebbins, G.L. (1958). The inviability weakness and sterility of interspecific hybrids. Adv. Genet. 9: 147-215.
  14. Thiyagu, K., Jayarnani, P. and Nadarajan, N., (2008), Pollen pistil interaction in interspecific crosses of Vigna sp. Cytologia. 73: 251-257.

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