Legume Research

  • Chief EditorJ. S. Sandhu

  • Print ISSN 0250-5371

  • Online ISSN 0976-0571

  • NAAS Rating 6.80

  • SJR 0.391

  • Impact Factor 0.8 (2024)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Legume Research, volume 43 issue 6 (december 2020) : 757-763

Morphological and Ultra-Structural Characterization of Blackgram [Vigna mungo (L.) Hepper] Genotypes Grown under High Temperature Stress

C. Partheeban, H. Vijayaraghavan
1Department of Crop Physiology, Tamil Nadu Agricultural University, Coimbatore-641 003, Tamil Nadu, India.

  • Submitted02-11-2019|

  • Accepted22-06-2020|

  • First Online 28-07-2020|

  • doi 10.18805/LR-4273

Cite article:- Partheeban C., Vijayaraghavan H. (2020). Morphological and Ultra-Structural Characterization of Blackgram [Vigna mungo (L.) Hepper] Genotypes Grown under High Temperature Stress. Legume Research. 43(6): 757-763. doi: 10.18805/LR-4273.

ABSTRACT

Background: In this decade, increasing temperatures and adverse environmental factors are expected to influence the crop yield, seed quality and germination ability. Prolonged heat stress affects the pod setting, seed morphology and alters the starch granules.
Methods: In this study, Scanning Electron Microscopy (SEM) and Light microscope was used to investigate the variations of morphological and ultra-structural characteristics in blackgram genotypes grown under high temperature (38+2°C) stress (HTS). Nineteen blackgram genotypes were evaluated under open top temperature controlled chamber (OTC) and the genotypes were classified into three categories such as heat tolerant, moderately heat tolerant and heat susceptible genotypes based on the Temperature Induction Response (TIR) and yield reduction percentage under HTS. Two genotypes were selected and examined the seed size, surface pattern, starch granule size and hilum from the each category.
Result: Seed coat morphology was captured using SEM and light microscope showed that the tolerant genotype VBG-07-001 had a clear shiny surface, thin and reduced cotyledon fissures, compact surface devoid of surface deposits and pits whereas rest of the genotypes (VBG-06-001, VBN-6, CoBg-11-02, CoBg-11-03 and VBG-08-003) had a rough surface. A bold and well-structured starch granule was observed in heat tolerant lines whereas, unstructured and pleated granules were observed in moderately tolerant and heat stress lines. These morphological and ultra-structural studies revealed remarked diversity among different genotypes that can be regarded as useful to screen the genotypic tolerant characters for HTS. 

REFERENCES

  1. Bhandari, K., Siddique, K.H., Turner, N.C., Kaur, J., Singh, S., Agrawal, S.K., et al. (2016). Heat stress at reproductive stage disrupts leaf carbohydrate metabolism, impairs reproductive function and severely reduces seed yield in lentil. Journal of Crop Improvement. 30: 118-151.
  2. Chaves, M.M., Flexas, J., Pinheiro, C. (2009). Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. Annals of Botany. 103(4): 551-560.
  3. Gandhi, D., Albert, S., Pandya, N. (2011). Morphological and micromorphological characterization of some legume seeds from Gujarat, India. Environmental and Experimental Botany. 9: 105-113.
  4. Gulgun Yildiz Tiryaki, Abdullah Cil and Iskender Tiryaki. (2016). Revealing Seed Coat Colour Variation and Their Possible Association with Seed Yield Parameters in Common Vetch (Vicia sativa L.). International Journal of Agronomy. http://dx.doi.org/10.1155/2016/1804108.
  5. Hasanuzzaman, M., Nahar, K., Alam, M.M., Roychowdhury, R. and Fujita, M. (2013). Physiological, biochemical and molecular mechanisms of heat stress tolerance in plants-Review. International Journal of Molecular Science. 14: 9643-    9684.
  6. Joseph, E., Crites, S.G., Swanson, B.G. (1993). Microstructure of black, green and red gram. Food Structure. 12: 155-156. 
  7. Julio Mercadera, Tolutope Akejuab, Melisa Brownc, Mariam Bundalaad, Matthew, J., Collinse, Les Copelandf, Alison Crowthergh, Peter Dunfieldb, Amanda Henryi, Jamie Inwooda, Makarius Itambuad, Joong-Jae Kimb, Steve Larterc, Laura Longoj, Thomas Oldenburgc, Robert Patalanoa, Ramaswami Sammynaikenk, María Sotoa, Robert Tylerl and Hermine Xhauflairm. (2018). Exaggerated expectations in ancient starch research and the need for new taphonomic and authenticity criteria, FACETS. 3: 777-798.
  8. Julio, M., Matthew, A., Robert, B., Mariam, B., Siobhan, C., Julien, F., Jamie, L., Makarius, I., Fergus, L., Patrick, L., Robert, P., Maria, S., Laura, T., Dale, W. (2018). Morphometrics of starch granules from sub-saharan plants and the taxonomic identification of ancient starch. Frontier in Earth Science. 6: 146.
  9. Kanak Sahai (2001). Anatomical variability in seed coat of some Cassia L. (Caesalpinioideae) species with taxonomic significance. Taiwania. 46(2): 158-166.
  10. Krishnamurthy, L., Gaur, P.M., Basu, P.S., Chaturvedi, S.K., Tripathi, S., Vadez, V, Rathore, A., Varshney, R.K., Gowda, C.L.L. (2011). Large genetic variation for heat tolerance in the reference collection of chickpea (Cicer arietinum L.) germplasm. Plant Genetic Resources. 9: 59-61.
  11. Kumar R Ranjeet, Sushil K Sharma, Suneha Goswami, Singh, G.P., Rajendra Singh, Kushboo Singh, Himanshu Pathak, Raj D Rai. (2013). Characterization of differentially expressed stress-associated proteins in starch granule development under heat stress in wheat (Triticum aestivum L.). Indian Journal of Biochemistry and Biophysics. 50: 126-138.
  12. Leila Mirzaei, Mostafa Assadi, Taher Nejadsatari, Iraj Mehregan. (2015). Comparative seed and leaf micromorphology of Colutea species (Fabaceae) from Iran. Environmental and Experimental Biology. 13: 183-187.
  13. Liu, P., Guo, W., Jiang, Z., Pu, H., Feng, C., Zhu, X., Peng, Y., Kuang, A. Little, C.R. (2011). Effects of high temperature after anthesis on starch granules in grains of wheat (Triticum aestivum L.). Journal of Agricultural Science. 149: 159-    169.
  14. Munier-Jolain, N.G. and Ney, B. (1998). Seed growth rate in grain legumes. II. Seed growth rate depends on cotyledon cell number. Journal of Experimental Botany. 49: 1971-1976.
  15. Nathalie Munier-Jolain and Christophe Salon. (2003). Can sucrose content in the phloem sap reaching field pea seeds (Pisum sativum L.) be an accurate indicator of seed growth potential. Journal of Experimental Botany. 54 (392): 2457-2465.
  16. Partheeban, C. and Vijayaraghavan, H. (2017). Evaluation of black gram [Vigna mungo (L.) hepper] genotypes under high temperature and interaction with elevated carbon dioxide. Research Journal of Recent Sciences. 6(1): 11-21.
  17. Partheeban, C., Vijayaraghavan, H., Sowmyapriya, S., Srividhya, S., Vijayalakshmi, D. (2017). Temperature induction response and accumulation of starch granules as indices to identify the thermotolerance of pulses at early growth stages. Legume Research. 40(4): 655-659.
  18. Ruchi Khedia, Manju Sharma, Sharma, K.P. Sharma, K.C. (2017). Scanning Electron Microscope Studies of Spermoderm Patterns of the Three Species of Vigna and their Cultivars. Research and Reviews: Research Journal of Biology. 5(2): 46-53.
  19. Sehgal, A., Sita, K., Kumar, J., Kumar, S., Singh, S., Siddique, K. H.M., et al. (2017). Effects of drought, heat and their interaction on the growth, yield and photosynthetic function of lentil (Lens culinaris Medikus) genotypes varying in heat and drought sensitivity. Frontier in Plant Science. 8: 1776. doi: 10.3389/fpls.2017.01776.
  20. Serrato, G.V., De Vries, M., Cornara, L. (1995). The Hilar Region in Leucaena leucocephala Lam. (De Wit) Seed: Structure, Histochemistry and the Role of the Lens in Germination. Annals of Botany. 75(6): 569-574.
  21. Sharma, L., Priya, M., Bindumadhava, H., Nair, R.M., Nayyar, H. (2016). Influence of high temperature stress on growth, phenology and yield performance of mungbean [Vigna radiata (L.) Wilczek] under managed growth conditions. Scientific Horticulture. 213: 379-391.
  22. Swanson, B.G., Hughes, J.S., Ramussen, H.P. (1985). Seed microstructure: Review of water imbibition in legumes. Food Microstructure. 4: 115-124. 
  23. Vijayalakshmi, D., Srividhya, S., Vivitha, P., Raveendran, M. (2015). Temperature induction response (TIR) as a rapid screening protocol to dissect the genetic variability in acquired thermotolerance in rice and to identify novel donors for high temperature stress tolerance. Indian Journal of Plant Physiology. 20(4): 368-374.
  24. Yang, H., Gu, X., Mengqiu, D., Weiping, L., Lu. D. (2018). Heat stress during grain filling affects activities of enzymes involved in grain protein and starch synthesis in waxy maize. Scientific Reports. 8: 15665.
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)