Legume Research

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Research Article

Legume Research, volume 44 issue 8 (august 2021) : 986-992

Development of Mapping Population and Validation of Molecular Markers Associated with MYMV Resistance in Mungbean

S.C. Mogali, B.N. Abhilash, L.G. Jaggal
1University of Agricultural Sciences, Dharwad- 580 005, Karnataka, India.

  • Submitted01-11-2019|

  • Accepted30-07-2020|

  • First Online 28-09-2020|

  • doi 10.18805/LR-4272

Cite article:- Mogali S.C., Abhilash B.N., Jaggal L.G. (2021). Development of Mapping Population and Validation of Molecular Markers Associated with MYMV Resistance in Mungbean. Legume Research. 44(8): 986-992. doi: 10.18805/LR-4272.

ABSTRACT

Background: Mungbean yellow mosaic virus disease is the most devastating disease on Mungbean production. The virus is transmitted by whitefly and can cause yield losses from 75 to 100 per cent. The development of mungbean cultivars resistant to both virus and its vector is considered as one of the most desirable means of managing the disease as it is environmentally safe and highly efficient. The selection of resistant genotypes in conventional methods is complex and time consuming. Hence, the use of molecular markers linked with resistance genes is powerful as it hastens the breeding programmes. The current study was aimed to develop mapping population and to validate molecular markers associated with Mungbean yellow mosaic virus (MYMV).
Methods: The present investigation was carried out with 260 F2 individuals that were derived from crossing DGGV-2 and IPM 2-14 during Kharif-2017 at Main Agril Research Station, UAS, Dharwad. Hybrid seeds of this cross were harvested individually and sown during rabi 2017 along with the two parents, as checks for distinguishing the true hybrids. Hybridity of F1s was confirmed through molecular marker analysis and the true F1s were selfed to raise the F2 generation.
Result: Of the 24 previously reported simple sequence repeat markers used for detecting the polymorphism, two markers viz., CEDG305 and CEDG115 were found to be polymorphic between DGGV-2 and IPM-2-14. Two hundred and sixty F2 plants segregated in the ratio of 3 S:1 R (202 susceptible: 58 resistant) as phenotypic and 1: 2 :1 as genotypic ratio implying that single recessive gene controlled resistance. Single marker analysis revealed that the molecular markers CEDG305 and CEDG115 were associated with MYMV resistance with a phenotypic variance of 24.5 and 10.3 per cent respectively.  

REFERENCES

  1. Agbagwa, I.O., Datta, S., Patil, P.G., Singh, P. and Nadarajan, N. (2012). A protocol for high quality genomic DNA extraction from legumes. Genet. Mol. Res. 11(4): 4632-4639. 
  2. Alam, A.M., Somta, P. and Srinives, P. (2014). Identification and confirmation of quantitative trait loci controlling resistance to mungbean yellow mosaic disease in mungbean. [Vigna radiata (L.) wilczek]. Mol. Breed. 34: 1497-1506. 
  3. Anonymous (2017). Annu. Rep. (2016-17), Directorate of Pulses Development, Ministry of Agriculture and Farmers Welfare, Gov. India, Vindhyachal Bhawan, New Delhi (India), p. 9. 
  4. Anusha, N. (2014). Identification of molecular markers linked to yellow mosaic virus resistance in greengram [Vigna radiate (L.) Wilczek]. Ph. D. Thesis, Acharya NG Ranga Agric. Univ., Rajendranagar, Hyderabad, Telangana (India). 
  5. Boling, M., Sander, D.A. and Matlock, R.S. (1961). Mungbean hybridization technique. Agron. J. 53: 54-55. 
  6. Chen, H.M., Ku, H.M., Schafleitner, R., Bains, T.S., Kuo, C.G., Liu, C.A. and Nair, R.M. (2013). The major quantitative trait locus for mungbean yellow mosaic India virus resistance is tightly linked in repulsion phase to the major bruchid resistance locus in a cross between mungbean [Vigna radiata (L.) Wilczek] and its wild relative Vigna radiata ssp. sublobata. Euphytica. 192(2): 205-216. 
  7. Dhole, V.J. and Reddy, K.S. (2013). Development of a SCAR marker linked with a MYMV resistance gene in mungbean [Vigna radiata (L.) Wilczek]. Plant Breed. 132(1): 127-132. 
  8. Gupta, S.K., Singh, R.A. and Chandra, S. (2005). Identification of a single dominant gene for resistance to mungbean yellow mosaic virus in blackgram [Vigna mungo (L.) Hepper]. J. Breed. Genet. 37(2): 85-89. 
  9. Gupta, S.K., Gupta, D.S., Anjum, T. K., Pratap, A. and Kumar, J. (2013). Inheritance and molecular tagging of MYMIV resistance gene in blackgram [Vigna mungo (L.) Hepper]. Euphytica. 193: 27-37. 
  10. Han, O.K., Kaga, A., Isemura, T., Wang, X.W., Tomooka, N. and Vaughan, D.A. (2005). A genetic linkage map for azuki bean [Vigna angularis (Willd.) Ohwiand Ohashi]. Theor. Appl. Genet. 111: 1278-1287. 
  11. Kitsanachandee, R., Somta, P., Chatchawankanphanich, O., Akhtar, K.P., Shah, T.M., Nair, R.M., Bains, T.S., Sirari, A., Srinives, P. and Kaur, L. (2013). Detection of quantitative trait loci for mungbean yellow mosaic India virus (MYMIV) resistance in mungbean [Vigna radiata (L.) Wilczek] in India and Pakistan. Breed. Sci. 63: 367-373. 
  12. Mohan, S., Sheeba, A., Murugan, E. and Ibrahim, S. M. (2014). Screening of mungbean germplasm for resistance to mungbean yellow mosaic virus under natural condition. Indian J. Genet. Plant Breed. 51(7): 891-896. 
  13. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York, 2: 145-151. 
  14. Singh, A.K. and Singh, K.P. (2000). Screening for disease incidence of yellow vein mosaic virus (YVMV) in okra [Abelmoschus esculentus (L.) Moench] treated with gamma rays and EMS. Veg. Sci. 27(1): 72-75. 
  15. Suman, S., Sharma, V.K., Kumar, H. and Shahi, V.K. (2018). Re-evaluation of the mungbean [Vigna radiata (L.) Wilczek] genotypes for resistance to mungbean yellow mosaic virus (MYMV) under screen-house conditions. Int. J. Curr. Microbiol. Appl. Sci. 7(4): 2821-2829. 
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