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Screening lentil (Lens culinaris Medik) Genotypes for Resistance against Pre-Flowering Blight and Identification of Pathogen by ITS Sequencing

DOI: 10.18805/LR-4248    | Article Id: LR-4248 | Page : 1493-1496
Citation :- Screening lentil (Lens culinaris Medik) Genotypes for Resistance against Pre-Flowering Blight and Identification of Pathogen by ITS Sequencing.Legume Research.2021.(44):1493-1496
Anirban Roy, Camellia Das, Moutushi Sarkar, Sourav Mondal, Shamba Ganguly, Sumit Kumar Murmu, Birendra Nath Panja, Rajib Nath, Kuldeep Tripathi, Prabir Kumar Bhattacharyya, Somnath Bhattacharyya
  smnthbhat@gmail.com
Address : Department of Genetics and Plant Breeding, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia-741 252, West Bengal, India. 
Submitted Date : 30-09-2019
Accepted Date : 8-02-2020

Abstract

Major constraints in increasing lentil growing area in eastern India is mortality due to several diseases throughout the growth stages. Breeding lentil for disease tolerance by genotypic resistance is most promising and easier method. Therefore, a collection of 500 genotypes have been screened in this study for pre-flowering blight-like disease which is predominant in experimental field for last ten years. Fungal DNA isolation from ten randomly selected diseased plants followed by ITS sequencing using universal primer pair revealed cent percent similarity in NCBI database with single organism Alternaria alternata. Three popular varieties viz. WBL-77, Ranjan and Asha were kept as check for disease reaction comparison. 466 genotypes survived till flowering and scoring was made using 1-9 scale. Only three lines, EC866132, IC267667, IC201778 established as the tolerant lines against Alternaria blight attack at the pre-flowering stage.

Keywords

Alternaria Blight ITS Lentil.

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