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Legume Research

Screening lentil (Lens culinaris Medik) Genotypes for Resistance against Pre-Flowering Blight and Identification of Pathogen by ITS Sequencing

DOI: 10.18805/LR-4248    | Article Id: LR-4248 | Page : 1493-1496
Citation :- Screening lentil (Lens culinaris Medik) Genotypes for Resistance against Pre-Flowering Blight and Identification of Pathogen by ITS Sequencing.Legume Research.2021.(44):1493-1496
Anirban Roy, Camellia Das, Moutushi Sarkar, Sourav Mondal, Shamba Ganguly, Sumit Kumar Murmu, Birendra Nath Panja, Rajib Nath, Kuldeep Tripathi, Prabir Kumar Bhattacharyya, Somnath Bhattacharyya
  smnthbhat@gmail.com
Address : Department of Genetics and Plant Breeding, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia-741 252, West Bengal, India. 
Submitted Date : 30-09-2019
Accepted Date : 8-02-2020
First Online:

Abstract

Major constraints in increasing lentil growing area in eastern India is mortality due to several diseases throughout the growth stages. Breeding lentil for disease tolerance by genotypic resistance is most promising and easier method. Therefore, a collection of 500 genotypes have been screened in this study for pre-flowering blight-like disease which is predominant in experimental field for last ten years. Fungal DNA isolation from ten randomly selected diseased plants followed by ITS sequencing using universal primer pair revealed cent percent similarity in NCBI database with single organism Alternaria alternata. Three popular varieties viz. WBL-77, Ranjan and Asha were kept as check for disease reaction comparison. 466 genotypes survived till flowering and scoring was made using 1-9 scale. Only three lines, EC866132, IC267667, IC201778 established as the tolerant lines against Alternaria blight attack at the pre-flowering stage.

Keywords

Alternaria Blight ITS Lentil.

References

  1. Bashi, E. and Rotem, J. (1974). Adaptation of four pathogens to semiarid habitats as conditioned by penetration rate germinating conidium survival. Phytopathology. 64:1035-1039.
  2. Bayaa, B., Erskine, W. and Singh, M. (1997). Screening lentil for resistance to Fusarium wilt: methodology and sources of resistance. Euphytica. 98:69-74.
  3. Das, R., Nath, R. and Dikshit H.K. (2017). Host resistance of lentil genotypes against Stemphylium blight caused by Stemphylium botryosum Wallr. In lower Ganzetic alluvial zone of West Bengal, India. Journal of Mycopathological Research. 55:169-172.
  4. Guharoy, S., Bhattacharyya, S., Mukherjee, S.K., Mondal, N. and Khatua, D. (2006). Phytophthora melonis associated with fruit and vine rot disease of pointed gourd in India as revealed by RFLP and sequencing of ITS region. J Phytopathology. 154:612-615. 
  5. Iqbal, A., Khalil, I.A., Ateeq, N. and Khan, M.S. (2006). Nutritional quality of important food legumes. Food Chem. 97:331-335.
  6. Kant, P., Materne1, M., Rodda M.S. and Slater, A.T. (2017). Screening lentil germplasm for Stemphylium blight resistance. Australasian Plant Pathol. 46:129-136.
  7. Kumar, P. (2007). Blight of lentil (Lens culinaris) in the cross Barimasur-    4 × Cdc milestone. Ph D-Thesis, Department of Plant Sciences, University of Saskatchewan, Saskatoon. 1-81.
  8. Mandal, D, Bhattacharyya, P., Das, R., Bhattacharyya, S. (2018). Diversity analysis by SSR markers and morphological markers among Stemphylium blight tolerance genotypes with high yielding cultivars of Lentil (Lens culinaris Medik). Legume Research. Online DOI: 10.18805/LR-4029.
  9. Meena, J.K., Singh, A., Dikshit, H. K., Mishra, G.P., Aski, M., Srinivasa, N., Gupta, S., Singh, D. and Tripathi, A. (2017). Screening of lentil (Lens culinaris Medikus sub sp. culinaris) germplasm against Fusarium Wilt (Fusarium oxysporum f. sp. lentil). Int. J. Curr. Microbiol. App. Sci. 6:2533-2541.
  10. Mwakutuya, E. (2010). Influence of temperature and wetness periods on the development of Stemphylium blight on lentil. Plant Disease. 94:1229-1224.
  11. Prasad, L., Dikshit, H. K., Aski, M.S. and Devi, T.P. (2017). First report of leaf spot of lentil caused by Alternaria tenuissima in India. Indian Phytopath. 70: 405.
  12. Pryor, B.M. and Gilbertson, R.L. (2000). Molecular phylogenetic relationships amongst Alternaria species and related fungi based upon analysis of nuclear ITS and mt SSU rDNA sequences. Mycol. Res.104:1312-1321.
  13. Thomma, B.P. (2003). Alternaria spp.: From general saprophyte to specific parasite. Molecular Plant Pathology. 4:225-236.
  14. Wheeler, B.E.J. (1969). An Introduction to Plant Disease and Fungi, John Wiley. Phytopathology. 22:837-845.

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