Identification and Molecular Characterization of Mung Bean Yellow Mosaic Virus in French Bean through Coat Protein Gene

DOI: 10.18805/LR-4234    | Article Id: LR-4234 | Page : 1198-1202
Citation :- Identification and Molecular Characterization of Mung Bean Yellow Mosaic Virus in French Bean through Coat Protein Gene.Legume Research.2021.(44):1198-1202
T.C. Archith, V. Devappa, B. Manjunath, Chirag Reddy archithf1@gmail.com
Address : Department of Plant Pathology, College of Horticulture, UHS campus, GKVK, Bengaluru-560 065, Karnataka, India.
Submitted Date : 10-09-2019
Accepted Date : 16-06-2020


Background: French bean (Phaseolus vulgaris L.), is one of the important legume vegetables grown in India. The disease mung bean yellow mosaic virus (MYMV) limits successful production of beans. MYMV has become an epidemic in bean growing regions, particularly in the tropical and subtropical countries. In summer, the disease is more serious and widely distributed and reported in various countries. The current study is aimed at identification of MYMV and molecular characterization of mung bean yellow mosaic virus in French bean. This study will be helpful for early detection of the virus, so that better preventive measures can be taken to control MYMV.
Methods: The investigations were conducted during 2016-2017, all the agro climatic zones of Karnataka province were surveyed. The coat protein (CP) gene of MYMV was amplified using gene specific primer with DNA isolated from MYMV infected leaves samples in french bean. Polymerase chain reaction products were Sequenced and analysed using a bioinformatic tool. 
Result: The CP gene decrypt sequences analysis revealed that the identity and similarity in global alignment for all the obtained sequences ranging from 80.8 to 95.3% with reference to MYMV, whereas for horse gram yellow mosaic virus (HYMV) it ranges from 90.4 to 99.1%. Two distinct yellow mosaic viruses infecting french bean (MYMV and HYMV) were identified and it was observed that there exists considerable genetic variation among these species. Present work showed that the CP region is efficient enough to provide a simple, rapid and reliable method for early detection of MYMV infections in french bean, which would help to develop proper management strategies to control these viruses.


Gemini virus HYMV MYMV Mung bean Whitefly Yellow mosaic disease


  1. Brown, J.K., Idris, A.M., Jerez, I.T., Banks, G.K., Wyatt, S.D. (2001). The core region of the coat protein gene is highly useful for establishing the provisional identification and classification of begomoviruses. Archives of Virology. 146:1581-1598 
  2. Chenulu, V.V., Venkateswarlu, V.,Rangaraju, R. (1979). Studies on yellow mosaic disease of mung bean.Indian Phytopathology. 32: 230-235.
  3. Deng, D., Mcgrath, P.F., Robinson, D.J., Harrison, B.D. (1994). Detection and differentiation of whitefly transmitted geminiviruses in plants and vector insects by the polymerase chain reaction with degenerate primers. Annals of Applied Biology.125: 327-336. 
  4. Fauquet., C.M. and Stanley, J. (2005). Geminivirus classification and nomenclature; progress and problems. Annals of Applied Biology. 142: 165-189.
  5. Haq, Q.M., Rouhibakhsh, A., Ali, A., Malathi, V.G. (2011). Infectivity analysis of a black gram isolate of mung bean yellow mosaic virus and genetic assortment with MYMV in selective hosts. Virus Genes. 6: 24-28.
  6. Jindal, P., Sohal, B.S., Bajaj, K.L., Jindal, P. (1998). Effect of uniconazole on the polyphenol metabolism in the leaves of mung bean (V. radiata, L. Wilczek) infected with mung bean yellow mosaic virus. Plant Physiology and Biochemistry. 25: 62-66. 
  7. Karthikeyan, A.S., Vanitharani, R., Balaji, V., Anuradha, S., Thillaichidambaram, P., Shivaprasad, P.V., Parameswari, C., Balamani, V., Saminathan, M., Veluthambi, K. (2004). Analysis of an isolate of Mungbean yellow mosaic virus (MYMV) with a highly variable DNA B component. Archives of Virology. 149: 1643-1652. 
  8. Krishna, T.G. and Jawali, N. (1997). DNA isolation from single or half seeds suitable for random amplified polymorphic DNA analysis. Analytical Biochemistry. 250: 125-127. 
  9. Mahajan, N., Parameswari, C., Veluthambi, K. (2011). Severe stunting in Black gram caused by the Mungbean yellow mosaic virus (MYMV) KA27 DNA B component is ameliorated by co- infection or post-infection with the KA22 DNA B: MYMV nuclear shuttle protein is the symptom determinant. Virus Research 157: 25-34.
  10. Marimuthu, T., Subramanian, C.L., Mohan, R. (1981). Assessment of yield losses due to yellow mosaic infection in mung bean. Pulse Crops News Letter. 1: 1-104. 
  11. Mayo, M.A. and Pringle, C.R. (1988). Virus Taxonomy-1997. Journal of General Virology.79: 649-657.
  12. Mohammad, N.I., Sonia, K.S., Rita. (2012). Molecular Characterization of Mungbean Yellow Mosaic Disease and Coat Protein Gene in Mungbean Varieties of Bangladesh.Plant Tissue Culture and Biotechnology. 22: 73 81.
  13. Morinaga, T., Ikegami, M., Miura, K. (1990). Physical mapping and molecular cloning of Mungbean yellow mosaic virus DNA. Intervirology. 31: 50-56.
  14. Obaiah, S., Bhaskara Reddy, B.V., Eswara Reddy, N.P., Siva Prasad, Y. (2013). Screening of some black gram (Vignamungo L. Hepper) genotypes for resistance to yellow mosaic virus. Current Biotica. 7: 96-100.
  15. Richa Maheshwari., Gatikrushna Panigrahi., Angappan. K. (2014). Molecular characterization of distinct YMV (Yellow mosaic virus) isolates affecting pulses in India with the aid of coat protein gene as a marker for identification. Molecular Biology Reports. 41: 2635-2644.
  16. Rybicki, E.P. (1994). A phylogenetic and evolutionary justification for three genera of Geminiviridae.Archives of Virology. 139: 49-77.
  17. Srivastava, H.K. (1979). Heterosis for chiasma frequencies and quantitative traits in common beans (Phaseolus vulgarisL.). Theoretical and Applied Genetics. 56: 25-29. 
  18. Varma, A., Dhar, A.K., Mandal, B. (1992). MYMV transmission and control in India. In: Green SK, Kim D (eds) Mungbean yellow mosaic disease. Asian Vegetable Research and Development Centre, Taipei. pp 8-27. 
  19. Wyatt, S.D. and Brown, J.K. (1996). Detection of subgroup III geminivirus isolates in leaf extracts by degenerate primers and polymerase chain reaction. Phytopathology. 86: 1288-1291. 

Global Footprints