Legume Research

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Research Article

Legume Research, volume 45 issue 1 (january 2022) : 32-38

Effect of Genetic Elimination of Kunitz Trypsin Inhibitor on Agronomic and Quality Traits in Soybean [Glycine max (L.) Merril]

Sandeep Kaur Dhaliwal, Satwinder Kaur Dhillon, B.S. Gill, Gurpreet Kaur, Asmita Sirari, Sunita Sharma
1Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana-141 001, Punjab, India.

  • Submitted30-07-2019|

  • Accepted09-03-2020|

  • First Online 22-08-2020|

  • doi 10.18805/LR-4205

Cite article:- Dhaliwal Kaur Sandeep, Dhillon Kaur Satwinder, Gill B.S., Kaur Gurpreet, Sirari Asmita, Sharma Sunita (2022). Effect of Genetic Elimination of Kunitz Trypsin Inhibitor on Agronomic and Quality Traits in Soybean [Glycine max (L.) Merril]. Legume Research. 45(1): 32-38. doi: 10.18805/LR-4205.

ABSTRACT

Background: Among the anti-nutritional factors present in soybean, kunitz trypsin inhibitor (Kti) serves as major anti-nutrient, retarding the activity of digestive proteases. Genetic removal of Kti allele to develop agronomically desirable genotypes with high nutritional value is major breeding objective in soybean.
Methods: The present study was performed on a set of 125 F5 genotypes derived from cross of SL525 (Kti +ve) and NRC101 (null Kti) to investigate the outcomes of genetic removal of Kti allele on characters of economic importance. 
Result: Comparison of mean of null Kti and Kti +ve plants for various parameters showed that introgression of null Kti allele adversely affected germination and grain yield. Germination, days to maturity, grain yield, plant height and seed weight were positively correlated with trypsin inhibitor activity and days to flowering, oil and protein content and fatty acid were unaffected by the allele present. The utilisation of the identified null kti genotypes would reduce extra cost on heat treatment incurred during soy processing and also boost utilization of soybean for bio-fortification in wheat flour to make chapattis.

REFERENCES

  1. A.O.A.C. (2000). Official Methods of Analysis. Association of Official Analytical Chemists International, Maryland, USA
  2. Appleqvist, L.A. (1968). Rapid methods of lipid extraction and fatty acid ester preparation for seed and leaf tissue with special remarks on preventing the accumulation of lipid contaminants. Ark Kenci, 28: 351-370.
  3. Arefrad, M., Babaian, J.N., Nematzadeh, G., Karimi, M., Kazemitabar, S.K. (2014). Assessment of seed storage protein composition of six Iranian adopted soybean cultivars [Glycine max (L.) Merrill.]. Journal of Plant Molecular Breeding. 2: 29-44. 
  4. Chen, H., Zhang, J., Neff, M.M., Hong, S.W., Zhang, H. (2008). Integration of light and abscisic acid signaling during seed germination and early seedling development. Proceedings of the National Academy Sciences of the United States of America. 105: 4495-4500. 
  5. Clemente, A., Marin-Manzano, M. C., Arques, M. C., Domoney, C. (2013). Bowman-birk inhibitors from legumes: Utilisation in disease prevention and therapy. In: Hemandez-Ledesma B, Hsieh CC (ed.) Bioactive food-peptides in health and disease, Intech Open Publisher, Pp 23-44 
  6. Friedman, M., Brandon, D.L., Bates, A.N., Hymowitzt, T. (2001). Nutritional and health benefits of soy proteins. Journal of Agricultural and Food Chemistry. 49: 1069-1086.
  7. Jofuku, K.D., Goldberg, R.B. (1989). Kunitz trypsin inhibitor genes are differentially expressed during the soybean life cycle and in transformed tabacco plants. Plant Cell. 1: 1079-1093.
  8. Kakade, M.L., Rackis, J.J., McGhee, J.E., Puskin, G. (1974). Determination of trypsin inhibitor of soy products: A collaborative analysis of an improved procedure. Cereal Chemistry. 51: 376-382.
  9. Khalil, I.A. and Manan, F. (ed) (1990). Colorimetry and Flame phptometry. In: Chemistry one (Bioanalytical chemistry). Taj printing press, Peshawar, Pakistan. Pp 131-57.
  10. Kunitz, M. (1945). Crystallization of a trypsin inhibitor from soybean science. Science. 101: 668-669.
  11. Liener, I. E. (1995). Possible adverse effects of soybean anticarcinogens. Journal of Nutrition. 125: 744-750.
  12. Marchetti, S., Delledonne, M., Fogher, C. (2000). Soybean kunitz, C-II and PI-IV inhibitor genes confer different levels of insect resistance to tobacco and potato transgenic plants. Theoretical of Applied Genetics. 101: 519-526.
  13. Moraes, R.M.A., Soares, T.C.B., Colombo, L.R., Salla, M.F.S., Barros, J.G., et al. (2006). Assisted selection by specific DNA markers for genetic elimination of the kunitz trypsin inhibitor and lectin in soybean seeds. Euphytica. 149: 221-226.
  14. Penfield, S., Josse, E.M., Kannangara, R., Gilday, A.D., Halliday, K.J. (2005). Cold and light control seed germination through the bHLH transcription factor SPATULA. Current Biology. 15: 1998-2006. 
  15. Rani, A., Kumar, V., Husain, S. M., Chauhan, G. M. (2010). NRC101 (IC0582901; INGR10054) and NRC102 (IC582902; INGR10055): Soybean (Glycine max) germplasm lines free from Kunitz inhibitor polypeptide. Indian of Journal Plant Genetic Resources. 24(1):122.
  16. Ryan, C.A. (1973). Proteolytic enzymes and their inhibitors in plants. Annual Review of Plant Physiology. 24: 173.
  17. Singh, T.P., Phul, P.S., Dhillon, S.K., Gill, B.S. (2005). SL525: A new yellow mosaic resistant soybean variety. Agriculture Research Journal. 42: 125. 
  18. Vesna, P., Mirjana, S., Vesna, D., Ana, N., Aleksandar, M., Snezana, M.D. (2014). Development of soybean varieties with specific nutritional composition of grain. Journal of Hygienic Engineering Design. 8: 174-177.
  19. Walling, L., Drews, G.N., Goldberg, R.B. (1986). Transcriptional and post-transcriptional regulation of soybean seed protein mRNA levels. Proceedings of the National Academy Sciences of the United States of America. 83: 2123-2127.
  20. Wilson, K.A., Papastoitsis, G., Hartle, P., Wilson, A.L.T. (1988). Survey of the proteolytic activities degrading the kunitz trypsin inhibitor and glycinin in germinating soybeans (Glycine max). Plant Physiology. 88: 355-360. 
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