Legume Research-An International Journal

Exploring the use of legumes as host plant species in Glomus mosseae sporulation

DOI: 10.18805/LR-419    | Article Id: LR-419 | Page : 913-918
Citation :- Exploring the use of legumes as host plant species in Glomus mosseae sporulation.Legume Research-An International Journal.2018.(41):913-918
Monther Mohumad Tahat, Kamaruzaman Sijam1 and Kholoud Alananbeh m.tahat@ju.edu.jo
Address : Department of Plant Protection, School of Agriculture, University of Jordan, Aljubeiha, Amman, 11942 Jordan.
Submitted Date : 29-03-2018
Accepted Date : 18-07-2018
First Online:

Abstract

The production of high quality, large scale, pathogen free and homogenous mycorrhizal inoculums are required for research purposes and soil bio-fertility. In the current study, a pot culture technique was followed to produce healthy quality and mass quantity of Glomus mosseae spores for research purposes. Five legumes plants were selected {(pea (Pisum sativum), broad bean, (Vicia faba) black eyed beans (Vigna unguiculata) soybean (Glycine max), and mung beans (Phaseolus aureus)}. The legumes were grown for two months under controlled conditions after pre-inoculated with healthy G. mosseae spores. The highest number of spores was counted in the mung beans rhizosphere and it was the best host in nodules weight production and root colonization rate. Soybeans produced the lowest spore’s number. It was found that the correlation between root colonization rate and spores number in the soil was positive. The rhizobium nodule weight was related positively with arbuscular mycorrhizal fungi spores manipulations in the soil.

Keywords

Black eyed beans Glomus mosseae Mung beans Mycorrhizal fungi Rhizobium.

References

  1. Al-Karaki, G., Mc-Michae, B. and John, Z. (2004). Field response of wheat to arbuscular mycorrhizal fungi and drought stress. Mycorrhiza,14:263–269
  2. Ballesteros-Almanza, L., J. Altamirano-Hernandez, J., Peña-Cabriales, J., Santoyo, G., Sanchez-Yañez, J.M., et al. (2010). Effect of Co-inoculation with mycorrhiza and rhizobia on the nodule trehalose content of different bean genotypes. The Open Microbiology. J.,4: 83–92
  3. Chaubey, A.K., Singh, H.P. and Shahi, U.P. (2015). Mycorrhizal strains efficacy in soybean. Agric. Sci. Digest., 35 (3):183-186.
  4. Clemow, S. R., Clairmont, L. Madsen, L.H. and Frédérique, C. G. (2011). Reproducible hairy root transformation and spot-inoculation methods to study root symbioses of pea. Plant Methods.7:46.2-15
  5. Cooper, A. J. (1975). Crop production in recirculating nutrient solutions. Sci.Hort. 3:251-258
  6. Douds, D.D. (1997). A procedure for the establishment of Glomus mosseae in a dual culture with Ri TDNA, transformed carrot roots. Mycorrhiza.7:57-61
  7. Douds, D.D., Nagahashi G., Pfeffer, P.E., Reider, C. and Kayser, W.M. (2006). On-farm production of AM fungus inoculum in mixtures of compost and vermiculite. Bioresour. Technol. 97:809-818
  8. Gerdman, J.W. and Nicolson, T.H. (1963). Spores of mycorrhizal Endogone Sp. extracted from soil by wet sieving and decanting. Trans. Br. Mycol. Soc. 46: 235-244
  9. Giovannetti, M. and Mosse, B. (1980). An evaluation of techniques to measure vesicular-arbuscular infection in the roots. New Phytol. 84: 489-500.
  10. Indriani , N. P , Yuwariah, Y., Rochana, A., Susilawati, I. and Khairani, L. (2016). The role of Vesicular Arbuscular Mycorrhiza (VAM) and rock phosphate application on production and nutritional value of centro legumes (Centrosema pubescens). Legume Research, 39 (6): 987-990.
  11. Jeffries, P., Gianinazzi, S., Perotto, S., Turnau, K., and Barea, J. M. (2003).The contribution of arbuscular mycorrhizal fungi in sustainable maintenance of plant health and soil fertility. Biol. Fert. Soils.37: 1-16
  12. Lugo, M.A., Maza, M.E.G. and Cabello, M.N. (2003). Arbuscular mycorrhizal fungi in a mountain grassland II. Seasonal variation of colonization studied, along with its relation to grazing and metabolic host type. Mycologia.95: 407-415
  13. Mortimera, P.E., Pe´rez-Ferna´ndezb, M.A. and Valentine, A.J. (2008). The role of arbuscular mycorrhizal colonization in the carbon and nutrient economy of the tripartite symbiosis with nodulated Phaseolus vulgaris. Soil Biol Biochem. 40: 1019–1027
  14. Mosse, B. (1962). The establishment of vesicular arbuscular mycorrhiza under aseptic conditions. J. Gen. Microbiol. 27: 509-520
  15. Philips, J. and Hayman, D.S. (1970). Improved procedure for clearing roots and staining parasitic and vesicular mycorrizal fungi for rapid assessment of infection. Trans. Br. Mycol. Soc. 55: 185-185
  16. Shi, Z.Y., Zhang, X.L., Xu, S.X. Lan, Z.J., Li, K.,Wang Y.M., Wang, F.Y. and Chen, Y.L. (2017). Mycorrhizal relationship in lupines: A review, Legume Research, 40(6): 965-973.
  17. Sivakumar, N. (2013). Effect of edaphic factors and seasonal variation on spore density and root colonization of arbuscular mycorrhizal fungi in sugarcane fields. Ann. Microbiol.63:151-160
  18. Smith, S.E. and Read D. J. (2008). Mycorrhizal Symbiosis. 3rd Edn., Academic Press, London.
  19. Smith, S.E. and Smith, F.A. (2011). Roles of arbuscular mycorrhizas in plant nutrition and growth: new paradigms from cellular to ecosystem scales. Annu. Rev. Plant. Biol.62:227-250
  20. Stancheva, I., Geneva, M., Zehirov. G., Tsvetkova, G., Hristozkov, M. and Georgiev, G. (2006). Effects of combined inoculation of pea plants with arbuscular mycorrhizal fungi and Rhizobiumon nodule formation and nitrogen fixing activity. Gen. Appl. Plant physiology. 32, 61-66
  21. Su, Y.Y., Sun. X. and Guo, L.D. (2011). Seasonality and host preference of arbuscular mycorrhizal fungi of five plant species in the Inner Mongolia steppe. China. Braz. J. Microbiol.42: 57-65
  22. Tahat, M.M. and Sijam, K. (2012). Mycorrhizal fungi and abiotic environmental conditions relationship. Research. J. Environ. Sci. 6: 125-133
  23. Tahat, M.M., Kamaruzaman, S., Radziah, O., Kadir, J., Masdek, H.N. (2008). Plant host selectivity for multiplication of Glomus mosseae spore. Int. J. Bot. 4:466–470.
  24. Temegne, N.C., Nkou-Foh T.D., Taffouo, V.D., Wakem, G.A. and Youmbi, E. (2018) Effect of mycorrhization and soluble phosphate on growth and phosphorus supply of Voandzou [Vigna subterranea (L.) Verdc.]. Legume Research, 41 (1): 1-6
  25. Thiagarajan, T.R., Ames, R.N. and Ahmad, M.H. ( 1992). Response of cowpea (Vigna unguiculata) to inoculation with co-selected vesicular arbuscular mycorrhizal fungi and rhizobium strains in field trials. Can. J. Microbiol. 38, 573-576
  26. Torrecillas, E., Alguacil, M.M. and Roldán, A. (2012). Host preference of arbuscular mycorrhizal fungi colonizing annual herbaceous plant species in semiarid Mediterranean prairies. Appl. Environ. Microbiol.78:6180-6186
  27. Vosatka, V. and Gryndler, M. (1999). Treatment with culture fraction from Pseudomonas putida modifies the development of Glomus fiscuatum mycorrhiza and the response of potato and maize plants to inoculation. Appl. Soil. Ecol. 11: 245-251
  28. Walker, C. and Vestberg, M.(1994). A simple and inexpensive method for producing and maintaining closed pot cultures of arbuscular mycorrhizal fungi. Agric. Sci. Finland. 3:233-240
  29. Wu, Q.S and Zou, Y.N. (2012). Evaluating effectiveness of four inoculation methods with arbuscular mycorrhizal fungi on trifoliate orange seedlings. Int. J. Agric. Biol.14: 266–270
  30. Wuen, K., Saito, K., Sato, S. and Sugawara, K. (2002). Arbuscular mycorrhizal colonization and sporulation in rhizosphere of common species on native and sown grasslands J. Jpn. Soc. Grassland Sci. 48: 248-253.
  31. Xiao, T.J., Yang,Q.S., Ran. W., Xu, G.H., and Shen, Q. R.(2010). Effect of inoculation with arbuscular mycorrhizal fungus on nitrogen and phosphorus utilization in upland rice-mungbean intercropping system. Agric. Sci. China. 9:528-535
  32. Zoble, R.W., Del-Tredici, P. and Torry, J.C. (1976). Methods for growing plants aeroponiccally. Plant Physiol. 57(3): 344-346. 

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