Legume Research

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Legume Research, volume 44 issue 7 (july 2021) : 743-750

Assessment of the Genetic Diversity of Black Gram [Vigna Mungo (L.) Hepper] Collections for Yellow Mosaic Virus Resistance Using Simple Sequence Repeat Markers 

N. Sathees, D. Shoba, S. Saravanan, S. Merina Prem Kumari, M. Arumugam Pillai
1Department of Plant Breeding and Genetics, Agricultural College and Research Institute, Killikulam, Vallanad, Tuticorin-628 252, Tamil Nadu, India.
  • Submitted16-04-2019|

  • Accepted11-09-2019|

  • First Online 03-12-2019|

  • doi 10.18805/LR-4155

Cite article:- Sathees N., Shoba D., Saravanan S., Kumari Prem Merina S., Pillai Arumugam M. (2019). Assessment of the Genetic Diversity of Black Gram [Vigna Mungo (L.) Hepper] Collections for Yellow Mosaic Virus Resistance Using Simple Sequence Repeat Markers. Legume Research. 44(7): 743-750. doi: 10.18805/LR-4155.
A total of nine black gram genotypes were tested for yellow mosaic virus (YMV) resistance along with other nine biometrical traits. The genotypes VBN 4, KKB-14-015, KKB-14-45 and KKB-14-022 exhibited complete resistance to YMV in field screening.  From the molecular characterization, out of 42 SSR markers studied, 15 SSR markers expressed polymorphism. The PIC values ranged from 0.37 (for SSR marker CEDG 024) to 0.79 (CEDG 154) with an average of 0.63. From the cluster analysis, YMV resistant genotypes KKB-14-045 and KKB-14-015 were located on clusters 6 and 7 and the susceptible but agronomic desirable genotypes IC 343943 and IC 436656 were located on cluster 3 which showed the diverse nature of these genotypes. Hence combinations viz., IC 343943 x KKB-14-015, IC 343943 x KKB-14-045, IC 436656 x KKB-14-015 and IC 436656 x KKB-14-045 would be ideal to produce desirable recombinants for YMV resistance breeding in black gram.
  1. Alice, D. and Nadarajan, N. (2007). Screening techniques and assessment methods for disease resistance. Department of Pulses, TNAU.
  2. Archana, V. and Jawali, N. (2007). Genetic variation and relatedness in Vigna unguiculata revealed by microsatellites. BARC Newsletter, 285.
  3. Bajpai, A., Srivastava, N., Rajan, S. and Chandra, R. (2008). Genetic Diversity and discrimination of mango accessions using RAPD and ISSR markers. Indian Journal of Horticulture. 65(4): 377- 382.
  4. Basamma, K., Salimath, P. M., Malagouda, P. and Suma, B. (2015). Genetics, molecular dissection and identification of high yielding disease resistant lines to MYMV in blackgram (Vigna mungo). Legume Research. 38: 851-854.
  5. Chaitieng, B., Kaga, A., Tomooka, N., Isemura, T., Kuroda, Y. and Vaughan, D. A. (2006). Development of a black gram [Vigna mungo (L.) Hepper] linkage map and its comparison with an azuki bean (Vigna angularis (Willd.) Ohwi and Ohashi) linkage map. Theoretical and Applied Genetics. 113(7): 1261-1269.
  6. Gupta, S. K., Jegadeesan, S. and Reddy, K.S. (2015). Validation of molecular markers linked to yellow mosaic virus disease resistance in diverse genetic background of black gram [Vigna mungo (L.)Hepper]. Electronic Journal of Plant Breeding. 6(3):755-763.
  7. Han, O., Kaga, A., Isemura, T., Wang., Tomooka, N. and Vaughan, D. (2005). A genetic likage map for azuki bean (Vigna angularis (wild.) ohwi and ohashi).Theoretical and Applied Genetics. 111(7):1278-1287
  8. Hari, Y., Rao, P. J. M., Balakrishna, K., Rao, V. T., Reddy, P. R. R. and Mahesh, U. (2017). Genetic diversity analysis among Yellow Mosaic Virus (YMV) resistant and susceptible varieties in mungbean (Vigna radiata L.) using SSR markers. International Journal of Agriculture Innovations and Research. 6: 2319-1473.
  9. Jeevitha, S., Karthikeyan, R., Vignesh, M., Malarkodi, A., Tirumalai, R., Nainu, A. J. and Murugan, S. (2018). Estimation of morphological and molecular genetic diversity in blackgram (Vigna mungo (L.) Hepper) under YMV hotspot regime. Horticultural Biotechnology Research. 4: 06-09.
  10. Naik, B. J., Anuradha, C., Kumar, P. A., Sreedhar, V. and Chary, S. (2017). Identification of simple sequence repeats (SSR) markers linked to yellow mosaic virus (YMV) resistance in black gram (Vigna mungo (L). Hepper). Agriculture Update. 12: 812-819.
  11. Nene, Y.L. (1972). A Survey of the Viral Disease of Pulse crops in Uttar Pradesh, G.B. Pant University of Agriculture and Technology, Pantnagar, Research Buletin. 4:191.
  12. Palaniappan, J and Murugaiah, S. (2012). Genetic diversity as assessed by morphological and microsatellite markers in green gram (Vigna radiata L.). African Journal of Biotechnology. 11(84): 15091-15097.
  13. Prasanthi, L., Bhaskara Reddy, B. V., Rani, K. R., Devi, R. M., Geetha, B., Prasad, Y. S. and Reddy, K. R. (2011). Development of RAPD/SCAR marker for yellow mosaic disease resistance in black gram. Legume Research. 34: 129-133.
  14. Priya, L., Arumugam Pillai, M. and Shoba, D. (2018). Genetic divergence, variability and correlation studies in black gram [Vigna mungo (L.) Hepper]. Legume Research, DOI: 10.18805/LR-4065
  15. Pulses revolution-From food to Nutritional Security. (2018). Ministry of Agriculture and Farmers Welfare, Department of Agriculture, Cooperation and Farmers Welfare, Government of India, pp.26.
  16. Pyngrope, A. H., Noren, S. K., Wricha, T., Sen, D., Khanna, V. K. and Pattanayak, A. (2015). Genetic diversity analysis of black gram [Vigna mungo (L.) Hepper] using morphological and molecular markers. International Journal of Applied and Pure Science and Agriculture. 1(8): 104-113.
  17. Rana, D.S., Dass, A., Rajanna, G.A. and Kaur, R. (2016). Biotic and abiotic stress management in pulses. Indian Journal of Agronomy. 61(4th IAC special issue):S238-S248.
  18. Rathi, Y.P.S. (2002). Epidemology, yield losses and management of major disease of kharif pulses in India. Proceedings Plant Pathology and Asian Congress of Mycology and Plant Pathology. Oct 1-4. University of Mysore. 
  19. Saghai-Maroof, M. A., Soliman, K. M., Jorgensen, R. A. and Allard, R. (1984). Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proceedings of the National Academy of Science. 81(24):8014-8018.
  20. Shoba, D., Manivannan, N. and Vindhiyavarman, P. (2010). Genetic diversity analysis of groundnut genotypes using SSR markers. Electronic Journal of Plant Breeding. 1(6): 1420-1425.
  21. Sivaprakash, K. R., Prashanth, S. R., Mohanty, B. P. and Parida, A. (2004). Genetic diversity of black gram (Vigna mungo) landraces as evaluated by amplified fragment length polymorphism markers. Current Science. 86: 1411-1416.
  22. Somta, P., Sommanas, W. and Srinives, P. (2009). Molecular diversity assessment of AVRDC–The World Vegetable Center elite-    parental mungbeans. Breeding science. 59(2): 149-157.
  23. Tamilzharasi, M., Vanniarajan, C., Karthikeyan, A., Souframanien, J., Arumugam Pillai, M. and Meenakshisundaram, P. (2018). Evaluation of urdbean (Vigna mungo) genotypes for mungbean yellow mosaic virus resistance through phenotypic reaction and genotypic analysis. Legume Research, DOI: 10.18805/LR-4035
  24. Tanksley, S. D.,Young, N. D., Paterson, A. H. and Bonierbale, M. W. (1989). RFLP mapping in plant breeding: new tools for an old science. Biotechnology. 7(3): 257.
  25. Vanniarajan, C., Ganeshram, S., Souframanien, J., Veni, K., Lavanya, S. A., and Kuralarasan, V. (2017). Gamma rays induced urdbbean [Vigna mungo (L.) Hepper] mutants with YMV resistance, good batter quality and bold seeded type. Legume Research, DOI:10 188051LR-3824.
  26. Zhang, M. C., Wang, D. M., Zheng, Z., Humphry, M. and Liu, C. J. (2008). Development of PCR based markers for a major locus conferring powdery mildew resistance in mungbean (Vigna radiata). Plant Breeding. 127(4):429-432. 

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