Legume Research

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Research Article

Assessment of the Genetic Diversity of Black Gram [Vigna Mungo (L.) Hepper] Collections for Yellow Mosaic Virus Resistance Using Simple Sequence Repeat Markers 

N. Sathees, D. Shoba, S. Saravanan, S. Merina Prem Kumari, M. Arumugam Pillai
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1Department of Plant Breeding and Genetics, Agricultural College and Research Institute, Killikulam, Vallanad, Tuticorin-628 252, Tamil Nadu, India.

  • Submitted16-04-2019|

  • Accepted11-09-2019|

  • First Online 03-12-2019|

  • doi 10.18805/LR-4155

Cite article:- Sathees N., Shoba D., Saravanan S., Kumari Prem Merina S., Pillai Arumugam M. (2019). Assessment of the Genetic Diversity of Black Gram [Vigna Mungo (L.) Hepper] Collections for Yellow Mosaic Virus Resistance Using Simple Sequence Repeat Markers. Legume Research. 44(7): 743-750. doi: 10.18805/LR-4155.

ABSTRACT

A total of nine black gram genotypes were tested for yellow mosaic virus (YMV) resistance along with other nine biometrical traits. The genotypes VBN 4, KKB-14-015, KKB-14-45 and KKB-14-022 exhibited complete resistance to YMV in field screening.  From the molecular characterization, out of 42 SSR markers studied, 15 SSR markers expressed polymorphism. The PIC values ranged from 0.37 (for SSR marker CEDG 024) to 0.79 (CEDG 154) with an average of 0.63. From the cluster analysis, YMV resistant genotypes KKB-14-045 and KKB-14-015 were located on clusters 6 and 7 and the susceptible but agronomic desirable genotypes IC 343943 and IC 436656 were located on cluster 3 which showed the diverse nature of these genotypes. Hence combinations viz., IC 343943 x KKB-14-015, IC 343943 x KKB-14-045, IC 436656 x KKB-14-015 and IC 436656 x KKB-14-045 would be ideal to produce desirable recombinants for YMV resistance breeding in black gram.

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