Legume Research

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Research Article

Molecular Characterization of Fusarium oxysporum Causing Chickpea Wilt by Internal Transcribed Spacer (ITS) Marker

Bharati Jadhav, Sarika. R. Bhalerao, Priya George
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1Department of Plant Biotechnology, VDCABT, Latur, Vasantarao Naik Marathawada Krishi Vidyapeeth, Parbhani-431 401, Maharashtra, India.

  • Submitted10-04-2019|

  • Accepted12-10-2019|

  • First Online 03-12-2019|

  • doi 10.18805/LR-4151

Cite article:- Jadhav Bharati, Bhalerao R. Sarika., George Priya (2021). Molecular Characterization of Fusarium oxysporum Causing Chickpea Wilt by Internal Transcribed Spacer (ITS) Marker. Legume Research. 44(7): 842-848. doi: 10.18805/LR-4151.

ABSTRACT

Fusarium wilt caused by Fusarium  oxysporum f. sp. ciceris is one of the serious disease causes tremendous loss to crop throughout the world and has assumed serious proportions in the recent years. Wilt affected chickpea plant roots samples were collected from forty eight different regions of Maharashtra. The collected samples were characterized by designed ITS primers. The Fusarium oxysporum f. sp. ciceris (Foc) showed about 302 bp amplicon when subjected to PCR with these primers. Further the amplified product was digested with five restriction enzymes viz. AluI, EcoRI, HaeIII, MboI and MspI and restriction fragment length polymorphism (RFLP) pattern were analysed. A dendrogram derived from ITS-RFLP analysis of the rDNA region divided the Foc isolates into four clusters. The maximum genetic distance 0.75 exhibited by five Foc isolates viz. Foc-30, Foc-36, Foc-24, Foc-25 and Foc-48 belonging to Shahartakli, Shelur, Nashik, Satara, Kolhapur regions respectively and found to be more diverse among the other Foc isolates. Whereas isolate no. Foc-20, Foc-21 and Foc-8 from Ambajogai, Karjat and Rahata shown minimum genetic distance (0.13). This showed the genetic variability among the Foc isolates collected from different regions of Maharashtra.   

REFERENCES

  1. Alymanesh, M.R., Falahatirastegar, M., Jafarpour, B., Mahdikhanimoghadam, E., (2009). Genetic diversity in the fungus Fusarium solani f. sp. cucurbitae race 1, the casual agent of root and crown rot of cucurbits in Iran, using molecular markers. Pakistan Journal of Biological Sciences. 12: 836–843.
  2. Barhate, B.G., Dake, G.N., Game, B.C. and Padule, D.N. (2006). Variability for virulence in Fusarium oxysporum f. sp. ciceri causing wilt of chickpea. Legume Research. 29(4): 308-310.
  3. Bhattacharjee, D., Mane, S. S. and Bhattacharjee, J. (2015). Molecular characterization of Fusarium oxysporum f. sp. ciceri using scar markers. An International Quarterly Journal of Life Sciences. 10(4): 2089-2097. 
  4. Booth, C. (1971). Fusarium nomenclature in the genus of Fusarium. Commonwealth Mycological Institute, Kew, Surrey. Pp. 27-35.
  5. Bryan, G. T. and Daniels, M. J. (1995). Comparison of Fungi within the Gaeumannomyces-Phialophora complex by analysis of ribosomal DNA sequence. Applied Environmental Microbiology. 61:681-689. 
  6. Cenis, J. L. (1992). Rapid extraction of fungal DNA for PCR amplification. Nucleic Acids Research. 20 (9):2380. 
  7. Das,S. and Shende, S.S. (2015). ITS marker for detection of Fuarium oxysporum causing safflower wilt. Thesis. VNMKV, Parbhani, Maharashtra.
  8. Datta, S., Chaudhari, R. G., Shamim, M. D. and Vishwa, D., (2011). Polymorphism in the internal transcribed spacer (ITS) region of the ribosomal DNA among different Fusarium species. Archives of Phytopathology and Plant Protection. 44: (6)558556. 
  9. Diguta, C.F., Vincent, B., Guilloux-Benatier, M., Alexandre, H. and Rousseaux, S. (2011). PCR ITS-RFLP: a useful method for identifying filamentous fungi isolates on grapes. Food Microbiology. 28(6):1145-1154.
  10. Dohroo, P. and Sharma, S. K. (1992). Variability in Fusarium oxysporum f. sp. zingiberi. Mycosciences. 3: 176-180. 
  11. Dubey, S. C., Singh, S. R. and Singh, B. (2010). Morphological and pathogenic variability of Indian isolates of Fusarium oxysporum f. sp. ciceris causing chickpea wilt. Archives of Phytopathology and Plant Protection. 43:174-90. 
  12. Dubey, S. C., Suresh, M., Singh, B. (2007). Evaluation of Trichoderma species against Fusarium oxysporum f.sp ciceris for integrated management of chickpea wilt. Biological Control. 40: 118-127. 
  13. Golakiya, B. B., Bhimani, M. D. and Akbari, L. F. (2018). Efficacy of different fungicides for the management of chickpea wilt (Fusarium oxysporum f. sp. ciceri). International Journal of Chemical Studies. 6 (2): 199-205. 
  14. Haware, M. P., Nene, Y. L. and Natarajan, M. (1996). Survival of Fusarium oxysporum f. sp. ciceri. Plant Diseases. 66:809-10.
  15. Ingle, A. P. (2017). Diversity and identity of Fusarium species occurring on fruits, vegetables and food grains. Nusantra Bioscience. 1(1):44-51.
  16. Kamel, A. Ibrahim, N. A., Mohmed, A. Abdel, S. Mohamed, S. and Joseph, A. (2003). PCR identification of Fusarium genus based on nucler ribosomal-DNA sequence data. African Journal of Biotechnology. 2 (4): 82-85. 
  17. Kumar, U. (2017). Integrated wilt disease management in chickpea. International Journal of Pure and Applied Biosciences. 5 (6): 981-984.
  18. Navas-Cortes, J. A., Hau, B. and Jimenez-Diaz, R. M. (2000). Yield loss in chickpea in relation to development of Fusarium wilt epidemics. Phytopathology. 90:1269-78. 
  19. Nazar, R.N., Hu, X., Schmidt, J., Culham, D. and Robb, J. (1991). Potential use of PCR-amplified ribosomal intergenic sequences in the detection and differentiation of Verticillium wilt pathogens. Physiological and Molecular Plant Pathology. 39(1): 1-11. 
  20. Nene, Y. L., Haware, M. P. and Reddi, M. V. (1981). Chickpea diseases, resistance screening techniques. International Crop Research Institute for the Semi-Arid Ttropics, Patancheru, India. 21:65-71.
  21. Nybom, H. and Hall H.K., (1991). Minisatellite DNA fingerprints can distinguish Rubus cultivars and estimate their degree of relatedness. Euphytica. 53: 107-114.
  22. O´Donnell, K. (1992). Ribosomal DNA internal transcribed spacers are highly divergent in the phytopathogenic ascomycete Fusarium sambucinum (Gibberella pulicaris). Current Genetics. 22: 213-220.
  23. Padwick, G. W. (1941). Report of the Imperial Mycologist Rep. Imperial Agricultural Research Institute, New Delhi. pp 94-101.
  24. Patra, S., Kumar, M., Biswas and Mahato, A. (2017). Sustainable management of chickpea wilt caused by Fusarium oxysporum f.sp. ciceri. International Journal of Pure and Applied Biosciences. 5 (1): 526-529. 
  25. Prasanthi, L., Reddy, B.B., Rani, K.R. and Naidu, P.H. (2009). Molecular marker for screening fusarium wilt resistance in pigeonpea [Cajanus cajan (L.) Millspaugh]. Legume Research. 32(1): 19-24.
  26. Rohlf, M. (1998). NTSYS-pc. Numerical taxonomy and multivariate analysis system, version 2.02i. Department of Ecology and Evolution. State University of New York, Setauket, USA
  27. Saxena, M. C. and Singh, K. B. (1987). The Chickpea C.A.B. Int. ICARDA. pp 250-252
  28. Sutherland, R., Altus, V., Alexander, A. and Berg, N. (2012). Pathogenicity associated genes in Fusarium oxysporum f. sp. cubense race 4. South African Journal of Science. 8: 109-112.
  29. Thakur, S.K. and Sirohi, A. (2009). Correlation and path coefficient analysis in chickpea (Cicer arietinum L.) under different seasons. Legume Research. 32(1):1-6.
  30. Welsh, J., Honeycut R.J., McClelland M. and Sorbral B.W.S., (1991). Parentage determination in maize hybrids using the arbitrarily primed polymerase chain reaction (AP-PCR). Theoretical and Applied Genetics. 82: 473-476. 
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