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Molecular Characterization of Fusarium oxysporum Causing Chickpea Wilt by Internal Transcribed Spacer (ITS) Marker

DOI: 10.18805/LR-4151    | Article Id: LR-4151 | Page : 842-848
Citation :- Molecular Characterization of Fusarium oxysporum Causing Chickpea Wilt by Internal Transcribed Spacer (ITS) Marker.Legume Research.2021.(44):842-848
Bharati Jadhav, Sarika. R. Bhalerao and Priya George bhartijadhav.2013@gmail.com
Address : Department of Plant Biotechnology, VDCABT, Latur, Vasantarao Naik Marathawada Krishi Vidyapeeth, Parbhani-431 401, Maharashtra, India.
Submitted Date : 10-04-2019
Accepted Date : 12-10-2019

Abstract

Fusarium wilt caused by Fusarium  oxysporum f. sp. ciceris is one of the serious disease causes tremendous loss to crop throughout the world and has assumed serious proportions in the recent years. Wilt affected chickpea plant roots samples were collected from forty eight different regions of Maharashtra. The collected samples were characterized by designed ITS primers. The Fusarium oxysporum f. sp. ciceris (Foc) showed about 302 bp amplicon when subjected to PCR with these primers. Further the amplified product was digested with five restriction enzymes viz. AluI, EcoRI, HaeIII, MboI and MspI and restriction fragment length polymorphism (RFLP) pattern were analysed. A dendrogram derived from ITS-RFLP analysis of the rDNA region divided the Foc isolates into four clusters. The maximum genetic distance 0.75 exhibited by five Foc isolates viz. Foc-30, Foc-36, Foc-24, Foc-25 and Foc-48 belonging to Shahartakli, Shelur, Nashik, Satara, Kolhapur regions respectively and found to be more diverse among the other Foc isolates. Whereas isolate no. Foc-20, Foc-21 and Foc-8 from Ambajogai, Karjat and Rahata shown minimum genetic distance (0.13). This showed the genetic variability among the Foc isolates collected from different regions of Maharashtra.   

Keywords

Chickpea Fusarium wilt ITS RFLP

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