Legume Research

  • Chief EditorJ. S. Sandhu

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Legume Research, volume 44 issue 5 (may 2021) : 527-538

Effect of Liquid and Charcoal Based Consortium Biofertilizers Amended with Additives on Growth and Yield in Chickpea (Cicer arietinum L.)

Anjali, Poonam Sharma, Sharon Nagpal
1Punjab Agricultural University, Ludhiana-141 004, Punjab, India.
  • Submitted04-03-2019|

  • Accepted21-10-2019|

  • First Online 03-12-2019|

  • doi 10.18805/LR-4131

Cite article:- Anjali, Sharma Poonam, Nagpal Sharon (2019). Effect of Liquid and Charcoal Based Consortium Biofertilizers Amended with Additives on Growth and Yield in Chickpea (Cicer arietinum L.). Legume Research. 44(5): 527-538. doi: 10.18805/LR-4131.
The present study was carried out to compare the shelf life and bio-efficacy of liquid and charcoal based biofertilizers of LGR33+RB1 amended with different additives viz.0.1% CMC, 2% PVP and 10mM trehalose stored at 4oC and 28oC upto 300 days in chickpea. Significantly high viable cell count of bacterial population as well as plant growth promoting (PGP) traits viz. IAA production and P solubilization at an interval of 30 days were recorded in liquid based biofertilizer treatments with additives during the entire study period (300 days) at 4oC and 28oC as compared to charcoal based biofertilizer treatments. However, among different additives amendment with 0.1% CMC showed maximum viable population and PGP traits in both liquid as well as charcoal based biofertilizers. In vivo studies, all liquid as well as charcoal based biofertilizer treatments with additives improved growth parameters in chickpea as compared to recommended consortium treatment without additives. Liquid biofertilizer treatment LGR33+RB1+0.1% CMC recorded maximum plant height, chlorophyll content, number of nodules, dry weight of nodules as well as  leghaemoglobin content, soil enzyme activity, total N and P content of shoot and soil along with improvement in grain yield over uninoculated control. 
  1. Alamraj E L D, Venkateswarlu B, Suseelendra D, Kumar G P, Ahmed S K M H, Meenakshi T, Sultan U, Pinisetty S and Narasu M L (2013). Effect of polymeric additives, adjuvants, surfactants on survival, stability and plant growth promoting ability of liquid bioinoculants. Journal of Plant Physiology and Pathol. 1: 2-12.
  2. Bashan Y, de-Bashan LE, Prabhu SR, Hernandez JP (2014). Advances in plant growth-promoting bacterial inoculant technology: formulations and practical perspectives. Plant Soil. 378:1-33.
  3. Brahmaprakash, G. P., Girisha, H. C., Navi, V., Laxmipathy, R. and Hedge, S.V. (2007). Liquid Rhizobium inoculant formulations to enhance biological nitrogen fixation in food legumes. Journal of Food Legumes. 20: 75-80.
  4. Buntić, A. V, Stajković-Srbinovi, O.S., Knežević, M. M., Kuzmanović, D. Z., Rasulić, N. V. and Delić, D. I. (2019). Development of liquid rhizobial inoculants and pre-inoculation of alfalfa seeds. Archives Biol Sci. 71: 379-387.
  5. Chandra, R. and Pareek, N. (2007). Comperative performance of liquid and carrier based inoculants in urdbean and mungbean. Journal of Food Legumes. 20: 80-82.
  6. Dixit, G. P. (2015). All India Coordinated Research Project on Chickpea. Indian Council of Agricultural Research, IIPR, Kanpur (U.P). pp 28.
  7. Fankem, H., Nwaga, D., Deubel, A., Dieng, L., Merbach, W. and Etoa, F.X. (2006). Occurrence and functioning of phosphate solubilizing microorganisms from oil palm tree (Elaeisguineensis) rhizosphere in Cameroon. African Journal of Biotechnology. 5: 2450-2460.
  8. Gopal, S. and Baby, A. (2016). Enhanced shelf-life of Azospirillum and PSB through addition of chemical additives in liquid formulations. International Journal of environmental science. 5: 2023-2029.
  9. Gupta, S. C. (2005). Evaluation of liquid and carrier based Rhizobium inoculants in chickpea. Indian J ournal of Pulses Research. 18: 40-42.
  10. Hedge, S. V. (2008). Liquid biofertilizers in Indian agriculture. Biofertilizer News Letter: 17-22.
  11. Kumaresan, G. and Reetha, D. (2011). Survival of Azospirillumbrasilense in liquid formulation amended with different chemical additives. Journal of Phytology. 3: 48-51.
  12. Meena, L. R., Singh, R. K. and Gautam, R .C. (2006). Response of chickpea (Cicer arietinum L.) to moisture conservation practices, phosphorus levels and bacterial inoculation under rainfed condition. International Journal of Tropical Agriculture. 22: 49-60.
  13. Mugilan, P., Gayathri, E. K., Elumalai, R. and Elango, R. (2011). Studies on improve survivability and shelf life of carrier using liquid inoculation of Pseudomonas striata. International Journal of Pharmaceutical and Bioliogical Archive. 2: 1271-1275.
  14. Namasivayam SKR, Saikia SL, Bharani ARS (2014). Evaluation of persistence and plant growth promoting effect of bioencapsulated formulation of suitable bacterial bio-fertilizers. Biosci Biotech Res Asia. 11: 407-415.
  15. Navi, V. (2004). Development of liquid inoculant formulations for Bradyrhizobium sp. (Arachis), Azospirillumlipoferum and Azotobacterchroococcum. Ph.D. Thesis, UAS, Banglore, India.
  16. Oad, F.C., Kumar, L. and Biswas, J. K. (2002). Effect of Rhizobium Japonicum inoculum doses (liquid culture) on the growth and seed yield of soybean crop. Asian Journal of Plant Science. 1: 340-342
  17. Pandey, S. K. Gupta. and Mukharjee, A. K. (2007). Impact of cadmium and lead on Catharanthus roseus- A phytoremediation study. Journal of Environmental Biology. 28: 655-662. 
  18. Pant, G. and Agrawal, P. K. (2014) Isolation and characterization of indole acetic acid producing plant growth promoting rhizobacteria from rhizospheric soil of Withaniasomnifera. Journal of Biological and Scientific Opinion. 2: 377-83.
  19. Prakash, O. (2010). Development and evaluation of liquid rhizobial inoculant technology for winter legumes. Ph.D. Thesis, CCSHAU, Hisar, India.
  20. Raja. and Takankhar, V. G. (2018) Response of liquid biofertilizers (Bradyrhizobium and PSB) on nutrient content in soybean (Glycine max L.). International Journal of Current Microbiology and Applied Sciences. 7: 3701-3706.
  21. Rigby, D. and Caceres, D. (2001). Organic farming and the sustainability of agricultural systems. Agricultural Systems. 68: 21-40.
  22. Sahai, P. and Chandra, R. (2009). Shelf life of liquid and carrier based Mesorhizobium sp. and Pseudomonas sp. inoculants under different storage conditions. Journal of Food legumes. 22: 280-282.
  23. Sahai, P. and Chandra, R. (2011). Performance of liquid and carrier- based inoculants of Mesorhizobium ciceri and PGP (Pseudomonas diminuta) in chickpea (Cicer arietinum L.) on nodulation, yield and soil properties. Journal of the Indian Society of Soil Science. 59(3): 263-267.
  24. Saharan, B .S. and Nehra, V. (2011). Plant growth promoting rhizobacteria: A critical review. Life Science and Medical Research. 2011: 1-30.
  25. Sehrawat, A., Suneja, S., Yadav, A. and Anand, R. C. (2015). Influence of different additives on shelf life of Rhizobial inoculants for mungbean (Vigna Radiata L.). International Journal of Recent Scientific Research. 6: 4338-4342.
  26. Sharma, P., Gupta, R. P. and Khanna, V. (2006). Evaluation of liquid Rhizobium inoculants in mungbean, urdbean and pigeonpea under field conditions. Indian Journal of Pulses Research. 19: 208-209.
  27. Shukla, M., Patel, R. H., Verma, R., Deewan, P. and Dotaniya, M. L. (2013). Effect of bio-organics and chemical fertilizers on growth and yield of chickpea (Cicer arietinum L.) under middle Gujarat conditions. Vegetos. 26: 183-187.
  28. Tabatabai, M. A. and Bremner, J. M. (1969). Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Boilogy and Biochemistry. 1: 301-307.
  29. Tagore, G. S., Namdeo, S. L. and Shah, S. K. (2013). Effect of microbial inoculants on nutrient uptake, yield and quality of chickpea genotypes. International Journal of Agricultural Sciences and Veterinary Medicine. 2: 19-23.
  30. Trivedi, M., Shanware, A. and Kalkar, S. (2016). Development of liquid formulation of native Rhizobium sp. for effective plant nourishment. International Journal Environmental and Agricuture Research. 2: 17-24.
  31. Ullah, S., Raza, M., Imran, M., Azeem, M., Awais, M. and Bilal, M .A. (2016). Plant growth promoting rhizobacteria amended with Mesorhizobiumciceriinoculation effect on nodulation and growth of chickpea (Ciceri arietinum). American Research Thoughts. 2: 3408-3420.
  32. Verma, J. P., Yadav, J. and Tiwari, K. N. (2010). Impact of plant growth promoting rhizobacteria on crop production. International Journal of Agricultural Research. 5: 954-983.
  33. Verma, J. P., Yadav, J., Tiwari, K. N. and Kumar, A. (2013). Effect of indigenous Mesorhizobiumspp. and plant growth promoting rhizobacteria on yields and nutrients uptake of chickpea (Cicer arietinum L.) under sustainable agriculture. Ecological Engineering. 51: 282-286.
  34. Wilson, D. O. and Reisenauer, H.M. (1963). Determination of leghaemoglobin in legume nodules. Analytical Biochemistry. 6: 27-30.
  35. Witham, P. H., Dyes, D. F. and Delvin, R. M. (1971). Chlorophyll absorption of spectrum and quantitative determination. Experiments in Plant Physiology, Von Nostr and Reinhoed Company, New York. pp. 51-56.
  36. Zommere, Z., Nikolajeva, V. (2017). Immobilization of bacterial association in alginate beads for bioremediation of oil-contaminated lands. Environmental and Experimental Botany. 15:105-11. 

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