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Research Article

volume 43 issue 4 (august 2020) : 470-479,   Doi: 10.18805/LR-4089

Studies on genetic diversity of French bean (Phaseolus vulgaris L.) landraces of Himachal Pradesh based on morphological traits and molecular markers

R
Rajesh Kanwar
D
D.K. Mehta
R
Rajnish Sharma
R
R.K. Dogra
1Department of Seed Science and Technology, College of Horticulture, Dr. Yashwant Singh Parmar University of Horticulture and Forestry Nauni, Solan-173 230, Himachal Pradesh, India.

  • Submitted09-10-2018|

  • Accepted28-02-2019|

  • First Online 14-08-2019|

  • doi 10.18805/LR-4089

Cite article:- Kanwar Rajesh, Mehta D.K., Sharma Rajnish, Dogra R.K. (2019). Studies on genetic diversity of French bean (Phaseolus vulgaris L.) landraces of Himachal Pradesh based on morphological traits and molecular markers. Legume Research. 43(4): 470-479. doi: 10.18805/LR-4089.

ABSTRACT

The knowledge of the extent of underlying diversity in the germplasm can be effectively employed to breed desirable cultivars and to challenge the consequences of the unprecedented biological, physical and chemical stresses of the future growing conditions. In the present study, total 26 geographically diverse French bean genotypes collected from different agro-climatic zones of Himachal Pradesh and two check varieties viz., Baspa and Jawala were grown under randomized complete block design with three replications for deciphering the extent of genetic diversity. The extent of variability was assessed using DUS morphological traits and PCR based approach. Analysis of variance for quantitative traits showed significant differences among all the genotypes for all the characters studied. The genotypes RL-19 and RL-20 gave higher seed yield and performed better for many of the horticultural traits over all the checks. Further, 15 RAPD primers were used for scoring polymorphism at the DNA level. The French bean landraces produced 79 PCR fragments, of which 59 were polymorphic with an average of 3.93 polymorphic fragments per primer. The overall percentage of polymorphism was recorded 74.62 per cent. The average Jaccard similarity index among genotypes ranged from 0.48 to 0.98, thereby showing sufficient genetic variability. Geographical affiliations and morphological associations were observed while studying the genotypic patterns within each group i.e. A, B, C and D. Such variability is desirable in breeding programs. Which also reveal the inherent capacity of the community as a selector, conservator and improver of the potential genetic resource for sustainable future.

REFERENCES

  1. Anonymous. (2011). Package of practices for kharif crops (in Hindi). Directorate of Extension Education, CSK Krishi Vishvavidyalaya, Palampur, Himachal Pradesh, pp. 58-60. 
  2. Anonymous. (2016-17). www.nhb.gov.in.
  3. Bhushan K.B., Singh B.P., Dubey R.K. and Ram H.H. (2007). Correlation analysis for seed yield in French bean (Phaseolus vulgaris L.). Pantnagar Journal of Research. 5(1): 104-106.
  4. Biswas M.S., Hassan J. and Hossain M.M. (2010). Assessment of genetic diversity in French bean (Phaseolus vulgaris L.) based on RAPD marker. African Journal of Biotechnology. 9(32): 5073-5077.
  5. Chiorato A.F., Carbonell S., Benchimol L.L., Chiavegato M.B., Dias L.A. and Colombo C.A. (2007). Genetic diversity in common bean accessions evaluated by means of morphoagronomical and RAPD data. Science and Agriculture. 64: 256-262.
  6. Das R., Thapa U., Debnath S., Lyngdoh Y.A. and Mallick D. (2014). Evaluation of French bean (Phaseolus vulgaris L.) genotypes for seed production. Journal of Applied and Natural Science. 6(2): 594-598.
  7. Doyle J.J. and Doyle J.L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin. 19: 11-15.
  8. Galindo L.M., Gaitan-Solis E., Baccam P. and Tohme J. (2004). Varietal performance, genetic variability and correlation in rajmash (Phaseolus vulgaris L.). Legume Research. 18: 223-227.
  9. Gomez K.A. and Gomez A.A. (1984). Statistical Procedure for Agricultural Research. New York: John Wiley. 690p.
  10. Jaccard P. (1908). Nouvelles researches surla distribution florale. Bulletin de la Societe Vaudoise Sciences Naturelles. 44: 223-270.
  11. Kar A., Gogoi H.K. and Gogoi P.J. (2006). Study on bean variability in Arunachal Pradesh. Bulletin of Arunachal Forest Research. 22(1&2): 40-44.
  12. Karasu A. and Oz M. (2010). A study on coefficient analysis and association between agronomical characters in dry bean (Phaseolus vulgaris L.). Bulgarian Journal of Agricultural Science. 16(2): 203-211.
  13. Kumar A., Singh P.K., Rai N., Bhaskar G.P. and Datta D. (2014). Genetic diversity of French bean (Phaseolus vulgaris L.) genotypes on the basis of morphological traits and molecular markers. Indian Journal of Biotechnology. 13: 207-213.
  14. Kumar P.A., Reddy R.V.S.K., Pandravada S.R., Durga R.C.V. and Chaitanya V. (2014). Genetic divergence studies in indigenous French bean (Phaseolus vulgaris L.) germplasm. Plant Archives. 14(1): 189-192.
  15. Kumar V., Sharma S., Sharma A.K., Kumar M., Sharma S., Malik S., Singh K.P., Sanger R.S. and Bhat K.V. (2008). Genetic diversity in Indian common bean (Phaseolus vulgaris L.) using random amplified polymorphic DNA markers. Physiology and Molecular Biology of Plants. 4: 383-387.
  16. Mavromatis A.G., Arvanitoyannis I.S., Korkovelos A.E., Giakountis A., Chatzitheodorou V.A. and Goulas C.K. (2010). Genetic diversity among common bean (Phaseolus vulgaris L.) Greek landraces and commercial cultivars:nutritional components, RAPD and morphological markers. Spanish Journal of Agricultural Research. 8(4): 986-994.
  17. Metais I., Aubry C., Hamon B., Jalouzot R. and Peltier D. (2000). Description and analysis of genetic diversity between commercial bean lines (Phaseolus vulgaris L.). Theoretical and Applied Genetics. 101: 1207-1214.
  18. Neupane R.K., Shrestha R., Vaidya M.L., Bhattarai E.M. and Daral R. (2005). Agro-morphological diversity in common bean (Phaseolus vulgaris L.) landraces of Jumla, Nepal. Proceeding of the Fourth International Food Legumes Research Conference (IFLRC-    IV). pp. 1-11. 
  19. Okii D., Tukamuhabwa P., Odong T. and Namayanja A. (2014). Morphological diversity of tropical common bean germplasm. African Crop Science Journal. 22(1): 59-67
  20. Prakash J. and Ram R.B. (2014). Genetic variability, correlation and path analysis for seed yield and yield related traits in French bean (Phaseolus vulgaris L.) under Lucknow conditions. International Journal of Innovative Science, Engineering & Technology. 1(6): 41-50.
  21. Prashanth N.D. (2003). Studies on spacing and phosphorus levels on seed yield and quality and varietal identification in French bean. M.Sc. (Agri.) Thesis, University of Agricultural Sciences, Dharwad, Karnataka, India. 
  22. Szilagyi L., Tayyar S. and Ciuca M. (2011). Evaluation of genetic diversity in common bean (Phaseolus vulgaris L.) using RAPD markers and morpho-agronomic traits. Romanian Biotechnological Letters. 16(1): 98-105.
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    Research Article

    volume 43 issue 4 (august 2020) : 470-479,   Doi: 10.18805/LR-4089

    Studies on genetic diversity of French bean (Phaseolus vulgaris L.) landraces of Himachal Pradesh based on morphological traits and molecular markers

    R
    Rajesh Kanwar
    D
    D.K. Mehta
    R
    Rajnish Sharma
    R
    R.K. Dogra
    1Department of Seed Science and Technology, College of Horticulture, Dr. Yashwant Singh Parmar University of Horticulture and Forestry Nauni, Solan-173 230, Himachal Pradesh, India.

    • Submitted09-10-2018|

    • Accepted28-02-2019|

    • First Online 14-08-2019|

    • doi 10.18805/LR-4089

    Cite article:- Kanwar Rajesh, Mehta D.K., Sharma Rajnish, Dogra R.K. (2019). Studies on genetic diversity of French bean (Phaseolus vulgaris L.) landraces of Himachal Pradesh based on morphological traits and molecular markers. Legume Research. 43(4): 470-479. doi: 10.18805/LR-4089.

    ABSTRACT

    The knowledge of the extent of underlying diversity in the germplasm can be effectively employed to breed desirable cultivars and to challenge the consequences of the unprecedented biological, physical and chemical stresses of the future growing conditions. In the present study, total 26 geographically diverse French bean genotypes collected from different agro-climatic zones of Himachal Pradesh and two check varieties viz., Baspa and Jawala were grown under randomized complete block design with three replications for deciphering the extent of genetic diversity. The extent of variability was assessed using DUS morphological traits and PCR based approach. Analysis of variance for quantitative traits showed significant differences among all the genotypes for all the characters studied. The genotypes RL-19 and RL-20 gave higher seed yield and performed better for many of the horticultural traits over all the checks. Further, 15 RAPD primers were used for scoring polymorphism at the DNA level. The French bean landraces produced 79 PCR fragments, of which 59 were polymorphic with an average of 3.93 polymorphic fragments per primer. The overall percentage of polymorphism was recorded 74.62 per cent. The average Jaccard similarity index among genotypes ranged from 0.48 to 0.98, thereby showing sufficient genetic variability. Geographical affiliations and morphological associations were observed while studying the genotypic patterns within each group i.e. A, B, C and D. Such variability is desirable in breeding programs. Which also reveal the inherent capacity of the community as a selector, conservator and improver of the potential genetic resource for sustainable future.

    REFERENCES

    1. Anonymous. (2011). Package of practices for kharif crops (in Hindi). Directorate of Extension Education, CSK Krishi Vishvavidyalaya, Palampur, Himachal Pradesh, pp. 58-60. 
    2. Anonymous. (2016-17). www.nhb.gov.in.
    3. Bhushan K.B., Singh B.P., Dubey R.K. and Ram H.H. (2007). Correlation analysis for seed yield in French bean (Phaseolus vulgaris L.). Pantnagar Journal of Research. 5(1): 104-106.
    4. Biswas M.S., Hassan J. and Hossain M.M. (2010). Assessment of genetic diversity in French bean (Phaseolus vulgaris L.) based on RAPD marker. African Journal of Biotechnology. 9(32): 5073-5077.
    5. Chiorato A.F., Carbonell S., Benchimol L.L., Chiavegato M.B., Dias L.A. and Colombo C.A. (2007). Genetic diversity in common bean accessions evaluated by means of morphoagronomical and RAPD data. Science and Agriculture. 64: 256-262.
    6. Das R., Thapa U., Debnath S., Lyngdoh Y.A. and Mallick D. (2014). Evaluation of French bean (Phaseolus vulgaris L.) genotypes for seed production. Journal of Applied and Natural Science. 6(2): 594-598.
    7. Doyle J.J. and Doyle J.L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin. 19: 11-15.
    8. Galindo L.M., Gaitan-Solis E., Baccam P. and Tohme J. (2004). Varietal performance, genetic variability and correlation in rajmash (Phaseolus vulgaris L.). Legume Research. 18: 223-227.
    9. Gomez K.A. and Gomez A.A. (1984). Statistical Procedure for Agricultural Research. New York: John Wiley. 690p.
    10. Jaccard P. (1908). Nouvelles researches surla distribution florale. Bulletin de la Societe Vaudoise Sciences Naturelles. 44: 223-270.
    11. Kar A., Gogoi H.K. and Gogoi P.J. (2006). Study on bean variability in Arunachal Pradesh. Bulletin of Arunachal Forest Research. 22(1&2): 40-44.
    12. Karasu A. and Oz M. (2010). A study on coefficient analysis and association between agronomical characters in dry bean (Phaseolus vulgaris L.). Bulgarian Journal of Agricultural Science. 16(2): 203-211.
    13. Kumar A., Singh P.K., Rai N., Bhaskar G.P. and Datta D. (2014). Genetic diversity of French bean (Phaseolus vulgaris L.) genotypes on the basis of morphological traits and molecular markers. Indian Journal of Biotechnology. 13: 207-213.
    14. Kumar P.A., Reddy R.V.S.K., Pandravada S.R., Durga R.C.V. and Chaitanya V. (2014). Genetic divergence studies in indigenous French bean (Phaseolus vulgaris L.) germplasm. Plant Archives. 14(1): 189-192.
    15. Kumar V., Sharma S., Sharma A.K., Kumar M., Sharma S., Malik S., Singh K.P., Sanger R.S. and Bhat K.V. (2008). Genetic diversity in Indian common bean (Phaseolus vulgaris L.) using random amplified polymorphic DNA markers. Physiology and Molecular Biology of Plants. 4: 383-387.
    16. Mavromatis A.G., Arvanitoyannis I.S., Korkovelos A.E., Giakountis A., Chatzitheodorou V.A. and Goulas C.K. (2010). Genetic diversity among common bean (Phaseolus vulgaris L.) Greek landraces and commercial cultivars:nutritional components, RAPD and morphological markers. Spanish Journal of Agricultural Research. 8(4): 986-994.
    17. Metais I., Aubry C., Hamon B., Jalouzot R. and Peltier D. (2000). Description and analysis of genetic diversity between commercial bean lines (Phaseolus vulgaris L.). Theoretical and Applied Genetics. 101: 1207-1214.
    18. Neupane R.K., Shrestha R., Vaidya M.L., Bhattarai E.M. and Daral R. (2005). Agro-morphological diversity in common bean (Phaseolus vulgaris L.) landraces of Jumla, Nepal. Proceeding of the Fourth International Food Legumes Research Conference (IFLRC-    IV). pp. 1-11. 
    19. Okii D., Tukamuhabwa P., Odong T. and Namayanja A. (2014). Morphological diversity of tropical common bean germplasm. African Crop Science Journal. 22(1): 59-67
    20. Prakash J. and Ram R.B. (2014). Genetic variability, correlation and path analysis for seed yield and yield related traits in French bean (Phaseolus vulgaris L.) under Lucknow conditions. International Journal of Innovative Science, Engineering & Technology. 1(6): 41-50.
    21. Prashanth N.D. (2003). Studies on spacing and phosphorus levels on seed yield and quality and varietal identification in French bean. M.Sc. (Agri.) Thesis, University of Agricultural Sciences, Dharwad, Karnataka, India. 
    22. Szilagyi L., Tayyar S. and Ciuca M. (2011). Evaluation of genetic diversity in common bean (Phaseolus vulgaris L.) using RAPD markers and morpho-agronomic traits. Romanian Biotechnological Letters. 16(1): 98-105.
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