Legume Research

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Research Article

Legume Research, volume 42 issue 2 (april 2019) : 270-276

The frequency of occurrence of pathogenic and saprotrophic fungi in pea seeds in different regions of Poland

Agnieszka Pszczó³kowska, Adam Okorski, Gabriel Fordoñski, Agnieszka Faligowska, Ewa Kaszkowiak, Jacek Olszewski, Alina Chareñska
1Department of Entomology, Phytopathology and Molecular Diagnostics, University of Warmia and Mazury in Olsztyn, Poland.

  • Submitted12-01-2018|

  • Accepted09-08-2018|

  • First Online 15-11-2018|

  • doi 10.18805/LR-405

Cite article:- Pszczó³kowska Agnieszka, Okorski Adam, Fordoñski Gabriel, Faligowska Agnieszka, Kaszkowiak Ewa, Olszewski Jacek, Chareñska Alina (2018). The frequency of occurrence of pathogenic and saprotrophic fungi in pea seeds in different regions of Poland. Legume Research. 42(2): 270-276. doi: 10.18805/LR-405.

ABSTRACT

Field pea seeds (cv. Tarchalska) grown in different regions of Poland (Warmia and Mazury, Wielkopolska, Kujawy) were subjected to mycological analyses. Simpson’s dominance index (DI) was used in the study to determine the abundance of each fungal species in the fungal communities isolated from seeds. PCR analyses were carried out with the use of species-specific primers for Fusarium fungi isolated from pea seeds to identify Tri genes responsible for trichothecene synthesis in the growing seasons of 2011, 2012 and 2013.Saprotrophic species predominated on pea seeds cv. Tarchalska grown in various Polish regions, whereas the isolated fungal pathogens were represented by species of the genus Fusarium and Botrytis cinerea. Genes encoding the synthesis of trichothecenes, the mycotoxins produced by Fusarium fungi, were not identified in the PCR analysis. In the group of peas grown in three Polish regions (Warmia and Mazury, Wielkopolska, Kujawy), the highest level of fungal colonization was noted in the pea seeds harvested in Warmia and Mazury. 

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