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Legume Research

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Research Article

Legume Research, volume 43 issue 5 (october 2020) : 711-717

Profiling the Seed Storage Protein Among Different Genotypes of Trigonella foenum-graecum L. (Fenugreek)

Nisha, Priyanka Khati, P.B. Rao
1Government P.G. College Syalde, Almora-263 661, Uttarkhand, India.

  • Submitted16-05-2018|

  • Accepted30-09-2019|

  • First Online 11-01-2020|

  • doi 10.18805/LR-4042

Cite article:- Nisha, Khati Priyanka, Rao P.B. (2019). Profiling the Seed Storage Protein Among Different Genotypes of Trigonella foenum-graecum L. (Fenugreek). Legume Research. 43(5): 711-717. doi: 10.18805/LR-4042.

ABSTRACT

A qualitative as well as quantitative categorization of seed storage proteins profiles of 23 genotypes of Trigonella foenum- graecum L. were performed by using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) for exploring the level of genetic discrepancy at seed storage protein level. Total soluble proteins were resolved on 10% resolving gel. A dendrogram was constructed on the basis of weight of seed storage proteins, which divide total genotypes into two groups further classified into different sub groups containing different genotypes in them. The bands obtained from gel electrophoresis can serve as a potent tool in discrimination of different genotypes on the basis of their protein content. Proteins with molecular weight 66, 43 and 35 kDa were found in all the genotypes except Fgk-76, PR, Rmt-303, PEB and Rmt-361, The 43 kDa protein band was found missing in Fgk-67, AFg-2, AM-2, AFg-4, Fgk-73, although the protein with 35 kDa weight was present in all the genotypes but not in Rmt-303 same as 63 kDa which is not present in Fgk-70 and 55 kDa  protein band was found missing in Fgk-67, Afg-4 and Rmt-361.

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