Profiling the Seed Storage Protein Among Different Genotypes of Trigonella foenum-graecum L. (Fenugreek)

DOI: 10.18805/LR-4042    | Article Id: LR-4042 | Page : 711-717
Citation :- Profiling the Seed Storage Protein Among Different Genotypes of Trigonella foenum-graecum L. (Fenugreek).Legume Research-An International Journal.2020.(43):711-717
Nisha, Priyanka Khati and P.B. Rao nishaarya24@gmail.com
Address : Government P.G. College Syalde, Almora-263 661, Uttarkhand, India.
Submitted Date : 16-05-2018
Accepted Date : 30-09-2019

Abstract

A qualitative as well as quantitative categorization of seed storage proteins profiles of 23 genotypes of Trigonella foenum- graecum L. were performed by using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) for exploring the level of genetic discrepancy at seed storage protein level. Total soluble proteins were resolved on 10% resolving gel. A dendrogram was constructed on the basis of weight of seed storage proteins, which divide total genotypes into two groups further classified into different sub groups containing different genotypes in them. The bands obtained from gel electrophoresis can serve as a potent tool in discrimination of different genotypes on the basis of their protein content. Proteins with molecular weight 66, 43 and 35 kDa were found in all the genotypes except Fgk-76, PR, Rmt-303, PEB and Rmt-361, The 43 kDa protein band was found missing in Fgk-67, AFg-2, AM-2, AFg-4, Fgk-73, although the protein with 35 kDa weight was present in all the genotypes but not in Rmt-303 same as 63 kDa which is not present in Fgk-70 and 55 kDa  protein band was found missing in Fgk-67, Afg-4 and Rmt-361.

Keywords

Genetic diversity Genotypes SDS-PAGE Seed Protein Trigonella foenum-graecum

References

  1. Ahmad, F., and Slinkard, A. E. (1992). Genetic relationships in the genus Cicer L. as revealed by polyacrylamide gel electrophoresis of seed storage proteins. Theoretical and Applied Genetics. 84(5-6): 688-692. 
  2. Balodi, B. (1991). The genus Trigonella L. (Fabaceae) in the northwest Himalaya. J. Econ. Tax. Bot. 5: 11-16.
  3. Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry. 72(1-2): 248-254.
  4. Budavari, S. (1996). The merck index: An encyclopedia of chemicals, drugs, and biological. 12th ed. N.J. Merk & Co, Inc., Whitehouse Station, NJ.
  5. De Britto, A. J., Kumar, P. B. J. R., and Gracelin, D. H. S. (2012). Genetic characterization of Abrus precatorius L. varieties using SDS-PAGE. Current Biotica. 5(3): 263-269.
  6. Dong J, Na Li, Hong De-lin (2006). Genetic diversity of seed Storage Proteins in different Ecotypes Varieties of japonica Rice and its application. Rice Science. 13(2): 85-92.
  7. Erum, Shazia, Anwar Rashid, and Shahid Masood. (2011). “evaluation of kasuri methi Trigonella foenum -graecum l. Var. To establish gi right of pakistan.” Pakistan Journal of Agricultural Research 24.1-4.
  8. Ferguson, J. M., and Grabe, D. F. (1986). Identification of cultivars of perennial ryegrass by SDS-PAGE of seed proteins 1. Crop science. 26(1): 170-176. 
  9. Gepts, P. (1990). Genetic diversity of seed storage proteins in plants. Plant population genetics, breeding, and genetic resources. 64-82.
  10. Haq, U.I., Nangyal1, H., Jan, G., Khan, S.M., Sadiqullah., Sherwani, K.S. and Kausar, R. (2014). Magnitude of genetic diversity of fenugreek (Trigonilla foenum–gracum) germplasm based on agronomic traits and biochemical markers. South Asian Journal of Life Sciences. 2 (2): 33 – 40.
  11. Hora, A., Chittora, M. and Malik, C. P. (2013). Estimation of genetic diversity based on seed storage proteins among different varieties of Trigonella foenum-graecum. LS: International Journal of Life Sciences. 2(1): 1-6.
  12. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227(5259): 680.
  13. Laroubi, A., Touhami, M., Farouk, L., Zrara, I., Aboufatima, R., Benharref, A., and Chait, A. (2007). Prophylaxis effect of Trigonella foenum graecum L. seeds on renal stone formation in rats. Phytotherapy Research. 21(10): 921-925. . 
  14. Naidu, M. M., Shyamala, B. N., Naik, J. P., Sulochanamma, G. and Srinivas, P. (2011). Chemical composition and antioxidant activity of the husk and endosperm of fenugreek seeds. LWT-Food Science and technology. 44(2): 451-456.
  15. Sushma, N., and Devasena, T. (2010). Aqueous extract of Trigonella foenum -graecum (fenugreek) prevents cypermethrin-induced hepatotoxicity and nephrotoxicity. Human and Experimental Toxicology. 29(4): 311-319. 
  16. Nemati, M., Bazdar, J., and Hokmalipour, S. (2012). Study of total seed storage protein in wheat varieties based on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Tech. J. Engin. App. Sci. 2(2): 39-43.
  17. Newall, C. A., Anderson, L. A., and Phillipson, J. D. (1996). Herbal medicines. A guide for health-care professionals. The pharmaceutical press.
  18. Niknam, V., Sharifizadeh, B., Ebrahimzadeh, H., Zarre, S., and Izadpanah, M. (2004). Comparative study of proteins in seed of some species of Trigonella from Iran. Iranian International Journal of Science. 5(1): 1-11.
  19. Palaniswamy, M., Pradeep, B. V., Sathya, R., & Angayarkanni, J. (2010). In Vitro Anti-plasmodial activity of Trigonella foenum–    graecum L. Evidence-Based Complementary and Alternative Medicine. 7(4): 441-445.
  20. Petropoulos, G.A. 2002. Fenugreek- The genus Trigonella, Taylor and Francis, London and New York. 10: 163-165.
  21. Raymond, J., Inquello, V., and Azanza, J. L. (1991). The seed proteins of sunflower: comparative studies of cultivars. Phytochemistry. 30(9): 2849-2856.
  22. Smith, J. S. C., and Smith, O. S. (1986). Environmental effects on zein chromatograms of maize inbred lines revealed by reversed-    phase high-performance liquid chromatography. Theoretical and applied genetics. 71(4). 607-612.
  23. Snehlata, H. S., and Payal, D. R. (2012). Fenugreek (Trigonella foenum-graecum L.): an overview. Int J Curr Pharm Rev Res. 2(4): 169-87. 
  24. Sofalian, O., Asgari, A., Shokrpour, M., and Zakarya, R. A. (2012). Relationship between storage protein banding pattern and growth type of some bread wheat varieties. Emirates Journal of Food and Agriculture. 24(4): 288.
  25. Turi, N. A., Farhatullah, F., Rabbani, M. A. R., Khan, N. U., Akmal, M., Pervaiz, Z. H., and Aslam, M. U. (2010). Study of total seed storage protein in indigenous Brassica species based on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-    PAGE). African Journal of Biotechnology. 9(45): 7595-7602.

Global Footprints