Legume Research

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Research Article

Legume Research, volume 43 issue 5 (october 2020) : 728-734

Evaluation of urdbean (Vigna mungo) genotypes for mungbean yellow mosaic virus resistance through phenotypic reaction and genotypic analysis

M. Tamilzharasi, C. Vanniarajan, A. Karthikeyan, J. Souframanien, M. Arumugam pillai, P. Meenakshisundram
1Department of Plant Breeding and Genetics, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai-625 104, Tamil Nadu, India.

  • Submitted03-05-2018|

  • Accepted27-11-2018|

  • First Online 13-02-2019|

  • doi 10.18805/LR-4035

Cite article:- Tamilzharasi M., Vanniarajan C., Karthikeyan A., Souframanien J., pillai Arumugam M., Meenakshisundram P. (2019). Evaluation of urdbean (Vigna mungo) genotypes for mungbean yellow mosaic virus resistance through phenotypic reaction and genotypic analysis. Legume Research. 43(5): 728-734. doi: 10.18805/LR-4035.

ABSTRACT

Mungbean yellow mosaic virus (MYMV) is a whitefly-transmitted major destructive virus affecting urdbean productivity in India. The objective of this research was to identify urdbean genotypes resistant against MYMV based on the phenotypic reaction and genotypic analysis. A total 48 urdbean genotypes were evaluated for resistance to MYMV by visual scoring of symptoms in the field under natural conditions. Disease severity was assessed using 0-9 rating scale, according to the mean disease score, the urdbean genotypes were categorized into five groups resistant (R, 14 genotypes), moderately resistant (MR, 4 genotypes), moderately susceptible (MS, 10 genotypes), susceptible (S, 18 genotypes) and highly susceptible (HS, 2 genotypes). These results were confirmed through genotyping based on MYMV-resistance tagged molecular markers CEDG180, ISSR8111357 and YMV1 FR. In addition, biochemical analysis was carried out in the genotypes of each category (R-HS). Results showed that MYMV resistance was significantly and positively correlated with the phytic acid and total phenol contents, whereas negative correlation was observed with total sugars in susceptible genotypes. The new identified genotypes (resistant sources) can be utilized in the urdbean breeding programme for improving resistance to MYMV.

REFERENCES

  1. Anjum, T., Gupta, K. S., and Datta, S. (2010). Mapping of mungbean yellow mosaic India virus (MYMIV) and powdery mildew resistant gene in black gram (Vigna mungo). Electronic Journal of Plant Breeding, 1:1148-1152.
  2. Balakrishnan, N. (2006). Influence of allelochemical contents in plants on the incidence of major pest of cotton. Indian J. Pl. Prot., 34: 202-205.
  3. Basamma, K., Salimath, P. M., Malagouda, P., and Suma, B. (2015). Genetics, molecular dissection and identification of high yielding disease resistant lines to MYMV in blackgram (Vigna mungo). Legume Res., 38: 851-854.
  4. Chen, H.M., Ku, H.M., Schafleitner, R., Bains, T.S., Kuo, G.S., Liu, C.A., Nair, R.M. (2012). The major quantitative trait locus for Mungbean yellow mosaic Indian virus resistance is tightly linked in repulsion phase to the major bruchid resistance locus in a cross between mungbean (Vigna radiata) and its wild relative Vigna radiata ssp. sublobata. Euphytica. 192: 205-216.
  5. Dhole, V. J. and Reddy, K. S. (2015). Genetic variation for phytic acid content in mungbean (Vigna radiata L. Wilczek). The Crop Journal, 3:157-162.
  6. Ganapathy, T., Kuruppiah, R and Gunasekaran, K (2003). Identifying the source of resistance for Mungbean Yellow Mosaic Virus (MYMV), urdbean leaf curl virus disease in urdbean (Vigna mungo). In Annual Meeting and Symposium on recent Dev.s in the diagnosis and Management of Plant Diseases for Meeting Global Challenges, December 18-20, 2003, Univ. of Agric. Sci., Dharwad, p.30.
  7. Gao, Y., Shang, C., Maroof, M.A.S., Biyashev, R.M., Grabau, E.A., Kwanyuen, P., Burton, J.W and Buss, G.R. (2007). A modified colorimetric method for phytic acid analysis in soybean. Crop Sci. 47:1797–1803. 
  8. Girish, K.R. and Usha, R. (2005). Molecular characterization of two soybean-infecting begomoviruses from India and evidence for recombination among legume-infecting begomoviruses from South-East Asia. Virus Res. 108:167176.
  9. Gopi, P., Satyanarayana, A., Rama Krishna, A. and Samba Siva Rao, K.R.S. (2016). Evaluation of black gram germplasm for resistance against YMV. J. of Plant Pathol., 7: 2157-7471.
  10. Gupta, S. K., Souframanien, J and Reddy, K. S. (2015). Validation of molecular markers linked to yellow mosaic virus disease resistance in diverse genetic background of black gram (Vigna mungo (L.) Hepper). Electron. J. of Plant Breed., 6: 755-763.
  11. Gupta, S., Gupta, D.S., Anjum, T.K., Pratap, A., Kumar, J. (2013). Inheritance and molecular tagging of MYMIV resistance gene in black gram (Vigna mungo L. Hepper). Euphytica, 193: 27-37.
  12. Gupta, S.K and Gopalakrishna, T. (2008). Molecular markers and their application in grain legumes Breed. J. of Food Legumes., 21: 1-14.
  13. Karthikeyan, A., Shobhana, V. G., Sudha, M., Raveendran, M., Senthil, N., Pandiyan, M., and Nagarajan, P. (2014). Mungbean yellow mosaic virus (MYMV): a threat to green gram (Vigna radiata) production in Asia. International Journal of Pest Management, 60: 314-324.
  14. Karthikeyan, A., Sudha, M., Pandiyan, M., Senthil, N., Shobhana, V.G., Nagarajan, P.( 2011). Screening of MYMV resistant mungbean (Vigna radiata (L) Wilczek) progenies through agroinoculation. Int J Plant Pathol. 2:115-125.
  15. Karuppanapandian, T., Karuppudurai, T., Sinha, P.B., Kamarul, H.A. and Manoharan. K. (2006). Genetic diversity in green gram (Vigna radiata) landraces analyzed by using random amplified polymorphic DNA (RAPD). African J. of Biotechnol., 5: 29-31.
  16. Maiti, S., Basak, J., Kundagrami, S., Kundu, A., Pal, A. (2011). Molecular marker-assisted genotyping of mungbean yellow mosaic India virus resistant germplasms of mungbean and urdbean. Mol Biotechnol. 47: 95104.
  17. Manivannan, N., Sethuraman, K., and Natarajan, S. (2001). Screening of green gram (Vigna radiata (L.) Wilczek) germplasm for yellow mosaic resistance. Legume Res., 24: 268-271.
  18. Mayee, C.D and Datar, V.V. (1986). Phytopathometry, Department of Plant Pathol., Marathawada Agric. Univ., Parbhani. Technical Bull. No.1: 145–46.
  19. Mohan, S., Sheeba, A., Murugan, E. and Ibrahim. S. M. (2014). Screening of mung bean germplasm for resistance to MYMV under Natural Condition. Indian J. of Sci. and Technol., 7: 891–896.
  20. Murugan, M and Dhandapani. (2007). Induced systemic resistance activates defence responses to interspecific insect infestation on tomato. J. Veg. Sci., 12:43-62.
  21. Prasanthi, L., Bhaskara Reddy, B. V., Rani, K. R., Devi, R. M., Geetha, B., Prasad, Y. S., and Reddy, K. R. (2011). Development of RAPD/SCAR marker for yellow mosaic disease resistance in blackgram. Legume Res.,34: 129-133.
  22. Raghuraman, M., Gupta, G.P. and Singh, R.P. (2004). Biochemical constituents imparting resistance in upland cotton (G. hirsutum) to whitefly (B. tabaci). Indian J. Agric. Sci., 74: 505-507. 
  23. Sadasivam and Manickam (1996) Biochemical methods 2nd edition, New Age International (p) Ltd. Publisher New Delhi P.12.
  24. Souframanien, J and Gopalakrishna ,T (2006). ISSR and SCAR markers linked to the MYMV resistance gene in black gram (Vigna mungo). Plant Breed., 125: 619-622.
  25. Taggar, G, K., Gill, R.S., Gupta, A.K. and Singh, S. (2014). Induced changes in the antioxidative compounds of V. mungo genotypes due to infestation by B. tabaci. J. Environ. Bio., 35: 1037-1047.
  26. Usharani, K.S., Surendranath, B., Haq, Q.M.R., Malathi, V.G. (2004). Yellow mosaic virus infecting soybean in Northern India is distinct from the species infecting soybean in southern and western India. Curr. Sci. 86: 845-850.
  27. Vanniarajan, C., Ganeshram, S., Souframanien, J., Veni, K., Lavanya, S. A., and Kuralarasan, V. (2017). Gamma rays induced urdbbean [Vigna mungo (L.) Hepper] mutants with YMV resistance, good batter quality and bold seeded type. Legume Rese. DOI:10    188051LR-3824. 
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