Genetic divergence studies through microsatellite markers in pigeonpea [Cajanus cajan (L) Millsp.]

DOI: 10.18805/LR-4022    | Article Id: LR-4022 | Page : 312-319
Citation :- Genetic divergence studies through microsatellite markers in pigeonpea [Cajanus cajan (L) Millsp.].Legume Research-An International Journal.2020.(43):312-319
Pankaj Sharma, Inderjit Singh, Asmita Sirari, Sarvjeet Singh and Gaurav Khosla pankaj-pbg@pau.edu
Address : Department of Plant Breeding and Genetics Punjab Agricultural University, Ludhiana-141 004, Punjab, India.
Submitted Date : 28-03-2018
Accepted Date : 5-05-2018

Abstract

The Genetic diversity was assessed among 96 pigeonpea accessions including 15 male sterile, 13 maintainer and 68 germplasm lines using 44 Simple Sequence Repeats (SSR) markers distributed over all the 11 chromosomes. Out of 44 SSR markers, 33 were polymorphic which showed 75% polymorphism among the used markers. For an individual primer, the alleles amplified varied from 2 to 4 with an average of 2.54. The Polymorphic Information Content (PIC) values ranged from 0.26 (CCM 0183) to 0.78 (CCM 0402 and CCM 0721). Based on 112 alleles amplified by SSR markers, the 96 genotypes were alienated into eight clusters. Cluster I and cluster VII were the largest with 22 genotypes each, cluster III and cluster IV were the smallest with two genotypes each, while cluster II, cluster V, cluster VI and cluster VIII consisted of 10, 15, 14 and 9 genotypes, respectively. Genotypes Pusa 991 and ULA 11 were found to be the most distant genotypes with highest dissimilarity coefficient (32%) where as AL 112A and AL 113A were the least distant genotypes with lowest dissimilarity coefficient (2%). Thus, highly distant genotypes can be used in pigeonpea improvement programs for getting desirable segregants. The selected panel of polymorphic SSR markers performed well in detection of genetic diversity patterns and can be used for future germplasm characterization studies in pigeonpea.

Keywords

Maintainer lines Male sterile Molecular diversity analysis Pigeonpea SSRs.

References

  1. Bohra, A., Dubey A., Saxena, R. K., Penmetsa, R. V., Poornima, K. N., Kumar, N., Farmer, A. D., Saxena, K. B., Kishor, (2011). Analysis of BAC-end sequences (BESs) and development of BES-SSR markers for genetic mapping and hybrid purity assessment in pigeonpea (Cajanus spp.). BMC Plant Biol, 11: 56.
  2. Bohra, A., Saxena, R. K., Gnanesh, B. N., Saxena, K. B., Byregowda, M., Rathore, A., et al (2012). An intra-specific consensus genetic map of pigeonpea [Cajanus cajan (L.) Millspaugh] derived from six mapping populations. Theor Appl Genet, 125: 1325-1338.
  3. Datta,J., Lal, N., Kaashyap, M. and Gupta, P. P. (2010). Efficiency of three PCR based marker systems for detecting DNA polymorphism in Cicer arietinum L and Cajanus cajan L. Millspaugh. Genet Eng Biotechnol J, 18: 1-5.
  4. FAOSTAT, (2016). Food and Agriculture Organization of the United Nations (FAO) Statistical Databases, http://faostat.fao.org/faostat/    en/#data/QC
  5. Jaggal, L.G., Patil, B. R., Naik, P. M. and Priya, K. (2016). Genetic diversity of selected accessions for seed protein among pigeonpea minicore collection. Legume Res, 39: 704-708.
  6. Kumar, C. V. S., Singh, I., Kumar, R. V., Patil, S., Tathineni, R., Mula, M. G., Saxena, R. K., et al (2016). Pigeonpea-a unique jewel in rainfed cropping systems. Legume Perspect, 11: 8-10.
  7. Lokesha Y. R., Muniswamy, G. G., Mahiboobsa, M. and Ramesh (2018). Molecular diversity and phenotypic characterization of A, B and R lines in Pigeonpea [Cajanus cajan (L.) Millsp.]. Legume Res, 41: 1-11.
  8. Manju, Y., Kumar, Y. Y., Pushpendra, K., Kumar, S. R., Renu, Y., Pawan, K., Shaily, J. et al (2017). Molecular diversity analysis as an improvement tool for pigeonpea [Cajanus Cajan (L.)].Res J Biotechnol, 12: 75-86.
  9. Murray, M. G. and Thompson, W. F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res, 8: 4321-4325
  10. Nadimpalli, R. G., Jarret, J. L., Pathak, S. C. and Kochert, G. (1993). Phylogenetic relationships of pigeonpea (Cajanus cajan) based on nuclear restriction fragment length polymorphism. Genome, 36: 216-223.
  11. Njunge, V., Deshpande, S., Siambi, M., Jones, R., Silima,S. and Villiers, S. D. (2016). SSR genetic diversity assessment of popular pigeonpea varieties in Malawi reveals unique fingerprints. Electron J Biotechnol, 21: 65-71.
  12. Odeny, A., Jayashree, B., Ferguson, M., Hoisington, D., Crouch, J. and Gebhardt, C. (2007). Development, characterization and utilization of microsatellite markers in pigeonpea. Plant Breed, 126: 130-136.
  13. Oinam, R., Jhansi, R. K., Anuradha, C., Jamaloddim, M. and Swathi. (2015). Genetic diversity study of pigeonpea (Cajanus cajan (L.) Millsp.) genotypes using SSR markers. Electron J Plant Breed, 6: 74-80.
  14. Panguluri, S. K., Janaiah, J., Govil, J. N., Kumar, P. A. and Sharma, P. C. (2006). AFLP fingerprinting in pigeonpea (Cajanus cajan L. Millsp.) and its wild relatives. Genet Res Crop Evol, 53: 523-531.
  15. Perrier, XandJacquemond-ColletJ.P.(2006). DARwin software. http://darwin.cirad.fr/darwin/
  16. Petchiammal, K. I., Muthiah, A. R. and Jayamani, P. (2015). Molecular characterization of CGMS, maintainer and inbred lines and diversity analysis in pigeonpea [Cajanus cajan (L.) Millsp.]. Legume Res, 38: 734-741.
  17. Pushpavalli, S. N. C. V. L., Rajeswari, R. R. and Kumar, C. V. S. (2016). Assessment of genetic diversity among pigeonpea male sterile    lines and popular cultivars using SSR markers. Electron J Plant Breed,7: 564-573.
  18. Ratnaparkhe, M. B., Gupta, V. S., Ven, Murthy. M. R. and Ranjekar, P. K. (1995). Genetic fingerprinting of pigeonpea (Cajanus cajan (L.) Millsp) and its wild relatives using RAPD markers. Theor Appl Genet, 91: 893-898.
  19. Sarkar, B., Chakravarthy, V. S. K., Varalaxami, Y., Yadav, S. K., Vanaja, M. and Maheswari, M. (2017). Genetic diversity among pigeonpea (Cajanus cajan L. Millspaugh) genotypes using genic SSRs with putative function for drought tolerance. Int J Curr Microbiol App Sci, 6:1804-1814.
  20. Saxena, R. K., Prathima, C., Saxena, K. B., Hoisington, D. A., Singh, N. K. and Varshney, R. K. (2010). Novel SSR markers for polymorphism detection in pigeonpea (Cajanus spp.). Plant Breed, 129: 142–148.
  21. Saxena, R. K., Penmetsa, R. V., Upadhyaya, H. D., Kumar, A., Carrasquilla, G. N., et al (2012). Large-scale development of cost effective single-nucleotide polymorphism marker assays for genetic mapping in pigeonpea and comparative mapping in legumes. DNA Res, 19: 449-461.
  22. Sheikh, W., Acharya, S., Patel, J. B., Kalaskar, S. R., Shinde, A. S. and Patel, K. A. (2015). Genetic fingerprinting of A and R lines of pigeonpea (Cajanus cajan (L.) Millsp.) using RAPD and SSR markers. Indian J of Biotechnol, 14: 328-333.
  23. Singh, A. K., Rai, V. P., Chand, R., Singh, R. P. and Singh, M. N. (2013). Genetic diversity studies and identification of SSR markers associated with Fusarium wilt (Fusarium udum) resistance in cultivated pigeonpea (Cajanus cajan). J Genet, 92: 273-280.
  24. Yang, S., Pang, W., Harper, J., Carling, J., Wenzl, P., Huttner, E., Zong, X. and Kilian, A. (2006). Low level of genetic diversity in cultivated pigeonpea compared to its wild relatives is revealed by diversity arrays technology (DArT). Theor Appl Genet, 113: 585-595. 

Global Footprints