Genetic diversity analysis of advanced breeding lines derived from interspecific and intervarietal crosses of black gram based on morphological and molecular markers

DOI: 10.18805/LR-3999    | Article Id: LR-3999 | Page : 480-487
Citation :- Genetic diversity analysis of advanced breeding lines derived from interspecific and intervarietal crosses of black gram based on morphological and molecular markers.Legume Research-An International Journal.2020.(43):480-487
R. Devi, R.K. Mittal, V.K. Sood, P.N. Sharma rajnidevi037@gmail.com
Address : Department of Crop Improvement, College of Agriculture, CSK Himachal Pradesh Agriculture University, Palampur-176 062, Himachal Pradesh, India.
Submitted Date : 2-02-2018
Accepted Date : 16-08-2018

Abstract

The main objective of this study was to assess the genetic diversity of 34 genotypes comprising of 25 advanced derivatives of interspecific cross between V. mungo x V. umbellata, five intervarietal crosses along with four checks (including blackgram parents) using 11 morphological traits and molecular markers (RAPD and ISSR) and for their resistance towards Cercospora, anthracnose and MYMV. The lines Palampur-93 x BRS-1 (236-A-L-1-4), HPBU-35 and HPBU-111 were found statistically at par to the best check Him Mash-1 for seed yield. Correlation studies revealed that the selection should be based on large seeds, more number of pods per plant and more number of seeds per pod indicating the importance of these traits in yield determination. The cluster analysis on molecular basis grouped the blackgram parents and advance lines differently than at morphological level which revealed genetic variation among genotypes and also confirmed that rigorous selection had been made for blackgram types in segregating generations rather than ricebean types as most of the advance derivatives of V. mungo x V. umbellata were found to be concentrated near blackgram parents. The lines PDU-1 x PRR-1 (62-3-L-10-1) and Palampur-93 x PRR-1 (258-1L-2-5) were found resistant to both Cercospora and anthracnose while, Palampur-93 x BRS-1 (236-A-L-3-2) for MYMV. The information generated from this study would be helpful in characterizing the advanced derived lines and parents in the selection and utilization of diverse genotypes to enhance variability and productivity along with resistance breeding of V. mungo.

Keywords

Black gram Genetic diversity Inter simple sequence repeat Random amplified polymorphic DNA V. mungo x V. umbellate.

References

  1. Anderson, J.A., Churchill, G.A., Sutrique, J.E., Tanksley, S.D. and Sorrells, M.E. (1993) Optimizing parental selection for genetic linkage maps. Genome 36:181-186. 
  2. Anonymous (2017). Commodity Profile for Pulses-March, 2017. http://agricoop.nic.in/sites/default/files/Pulsespdf.
  3. Belaj, A., Munoz-Diez, C., Baldoni, L., Porceddu, A., Barranco, D. and Satovic, Z. (2007). Genetic diversity and population structure of wild olives from North-Western Mediterranean assessed by SSR markers. Ann Bot 100:449-458.
  4. Dikshit, H.K., Jhang, T. Singh, N.K., Koundal, K.R., Bansal, K.C., Chandra, N., Tickoo, J.L. and Sharma, T.R. (2007). Genetic differentiation of Vigna Species by RAPD, URP and SSR Markers. Biologia Plantarum 3: 451-457.
  5. Goyal, P., Jain, R., Kachhwaha, S., and Kothari, S.L. (2015). Assessment of genetic diversity in Pithecellobium dulce (Roxb.) Benth. germplasm using RAPD and ISSR markers. Trees 29:637–653.
  6. Karuppanapadian, T., Wang, H.W., Karuppudurai, T., Rajendhran, J., Kwan, M., Jang, G.S., Kim, S.H., Manoharan, K., and Kim, W. (2010). Efficiency of RAPD and ISSR markers in assessing genetic diversity and relationships in blackgram (Vigna mungo (L.) Hepper) varieties. Can J Plant Sci 90:443-452.
  7. Marappa, N. (2008). Screening of mungbean genotypes and its wild relatives for resistant sources to Cercospora leaf spot disease. Asian Journal of Bio Science 3:324-326.
  8. Mayee, C.D. and Datar, V.V. (1986). Phytopathometry. Technical Bulletin-1 (Special bulletin-3), Marathwada Agricultural University, Parbhani, Maharastra, India. Pp 74-76.
  9. Mondini, L., Noorani, A., and Pagnotta, M.A. (2009). Assessing plant genetic diversity by molecular tools. Diversity 1:19–35.
  10. Murray, M.G. and Thompson, W.F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321-4325.
  11. Perrier, X. and Jacquemoud-Collet, J.P. (2006). DARwin software http://darwin.cirad.fr/.
  12. Rani, Y.U. and Rao, J.S. (1981). Path analysis of yield components in blackgram. Indian J. Agril. Sci 51: 378-381. 
  13. Rao, L.S., Usha Rani, P., Deshmukh, P.S., Kumar, P.A., Panguluri, S.K. (2007). RAPD and ISSR fingerprinting in cultivated chickpea (Cicer arietinum L.) and its wild progenitor Cicer reticulatum Ladizinsky. Genet Resour Crop Ev 54:1235-1244.
  14. Ravi, M., Geethanjali, S., Sammeyafarheen, F. and Maheswaran, M. (2003). Molecular marker based genetic diversity analysis in rice (Oryza sativa L.) using RAPD and SSR markers. Euphytica 133: 243-253.
  15. SAS (2012) Base SAS® 9.4, SAS Institute Inc., Cary, NC, USA.
  16. Sharma, K., Agarwal, V., Gupta, S., Kumar, R. and Prasad, M. (2008). ISSR marker-assisted selection of male and female plants in promising dioecious crops: jojoba (Simmondsia chinensis). Plant Biotechnol Rep 2:239-243.
  17. Singh, I., Sandhu, J.S. and Singh, S. (2013). Introgression of productivity and other desirable traits from ricebean (Vigna umbellata) into black gram (Vigna mungo). Plant Breed 132:401–406.
  18. Sood, V.K., Rana, I. and Hussian, W. (2014). Genetic diversity of genus Avena from North-Western Himalayas using molecular markers. In: Proceedings of National Academy of Sciences, India Section B: Biological Sciences 86:151–158.
  19. Souframanien, J. and GopalaKrishna, T. (2004). A comparative analysis of genetic diversity in blackgram genotypes using RAPD and ISSR markers. Theor Appl Genet 109:1687-1693.
  20. Vishalakshi, B., Umakanth, B., Shanbhag, A.P., Ghatak, A., Sathyanarayanan, N., Madhav, M.S., Gopala Krishna, G. and Yadla, H. (2017). RAPD assisted selection of black gram (Vigna mungo L. Hepper) towards the development of multiple disease resistant germplasm. 3 Biotech 7:1.
  21. Waugh, R. and Powell, W. (1992). Using RAPD markers for crop improvement. Trends Biotechnol 10:186-191. 

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