Ameliorative effect of thiourea priming on germination characteristics of mungbean (Vigna radiata L.) under water and salinity stress

DOI: 10.18805/LR-3966    | Article Id: LR-3966 | Page : 353-358
Citation :- Ameliorative effect of thiourea priming on germination characteristics of mungbean (Vigna radiata L.) under water and salinity stress.Legume Research-An International Journal.2020.(43):353-358
Shalini Jhanji and Madhu Dhingra shalinijhanji@pau.edu
Address : Punjab Agricultural University Ludhiana-141 004, Punjab, India.
Submitted Date : 17-11-2017
Accepted Date : 2-02-2018

Abstract

The germination behaviour of unsoaked, hydroprimed and thiourea primed seeds (TU,750, 1000 and 1250 ppm) in water , polyethylene glycol (PEG, -0.2 and -0.4 MPa ) and NaCl (30 and 50 mM) was investigated. The percent germination and seedling growth of TU primed seeds was best compared to other treatments under stressed conditions. The root/shoot ratio increased with stress in all seedlings and vice versa for seedling vigour index. TU primed seeds exhibited the highest tolerance index (85) under - 0.2 MPa PEG treatment and 40 tolerance index under 30mM salinity stress. Priming with thiourea @1000 ppm was the most effective in ameliorating water and salinity stress.

Keywords

Germination Mungbean Priming Salinity stress Thiourea Water stress.

References

  1. Abdelkader, A.F., Aronsson, H., Solymosi, K., Böddi, B. and Sundqvist, C. (2007): High salt stress induces swollen prothylakoids in dark-grown wheat and alters both prolamellar body transformation and reformation after irradiation. J. Exp. Bot. 58:2553-    2564.
  2. Abdul baki, A.A. and Anderson, J.D. (1973). Vigour determination in soybean seeds by multiple criteria. Crop Sci. 13(2): 630-633.
  3. Anjum, F., Wahid, A., Farooq, M. and Javed, F. (2011). Potential of foliar applied thiourea in improving salt and high temperature tolerance of bread wheat (Triticum aestivum). Int. J. Agric. Biol. 13 : 251-256.
  4. Ashraf, M. (2009). Biotechnological approach of improving plant salt tolerance using antioxidant as markers. Biotechnol. Adv. 27:84-93.
  5. Ashraf, M. and Foolad, M.R. (2007). Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ. Exp. Bot. 59:206-216.
  6. Ashraf, M.Y.,Akhtar, K., Sarwar, G. and Ashraf,M. (2002). Evaluation of arid and semi-arid ecotypes of guar (Cyamopsis tetragonoloba L.) for salinity (NaCl) tolerance. J. Arid Environ.52:437-482.
  7. Bano, A. and Aziz,N. (2003). Salt and drought stress in wheat and the role of ABA. Pak. J. Bot. 35:871-883.
  8. Bethke, P.C., Badger, M.R. and Jones, R.L. (2004). Apoplastic synthesis of nitric oxide by plant tissue. Pl. Cell 16:332-341.
  9. Bewley, J.D. and Black, M. (1982). Physiology and Biochemistry of Seed in Relation to Germination: Viability, Dormancy and Environmental Control. Berlin: Springer-Verlag, 375p.
  10. Das, P., Nutan, K.K., Singla-Pareek, S.L. and Pareek A. (2015). Oxidative environment and redox homeostasis in plants: dissecting out significant contribution of major cellular organelles. Front. Environ. Sci. 2:70.
  11. Dash, M. and Panda, S. K. (2001). Salt stress induced changes in growth and enzyme activities in germinating Phaseolus muingo seeds. Biol. Plant44: 587-589. 
  12. Demirevska, K., Simova-Stoilova, L., Vassileva, V., Vaseva, I., Grigorova, B. and Feller U.(2009). Drought-induced leaf protein alterations in sensitive and tolerant wheat varieties. Gen. App. Plant Physiol. 34:79-102.
  13. Devi, S., Patel, P.T. and Choudhary, K.M. (2015). Effect of application of SH-compounds on yield, protein and economics of summer green gram [Vigna radiata (L.) WILCZEK] under moisture stress in north Gujarat conditions. Legume Res. 38(4): 542-545.
  14. Farooq, M., Kobayashi, N., Wahid,A., Ito, O. and Basra, S.M.A. (2009a). Strategies for producing more rice with less water. Adv. Agron.101:351-387.
  15. Farooq, M., Wahid,A., Kobayashi,N., Fujita,D. and Basra,S.M.A. (2009b). Plant drought stress: effects, mechanisms and management. Agron. Sustain. Dev. 28: 185-212.
  16. Ingram, J. and Bartlets, D.(1996). The molecular basis of dehydration tolerance in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol.47:377-403.
  17. Iqbal, M.Z. and Rahmati,K. (1992). Tolerance of Albizia lebbeck to Cu and Fe application. Ekologia (CSFR) 11: 427-430.
  18. Kabar, K. and Beltepe, S. (1989). Effect of kinetin and gibberellic acid in overcoming high temperature and salinity (NaCl) stresses on the germination of barley and lettuce seeds. Phyton(Buenos Aires ) 30: 65-74.
  19. Khan, M.A. and Ungar, I.A. (2001). Alleviation of salinity stress and the response to temperature intwo seed morphs of Halopyrum mucronatum (Poaceae). Aust. J. Bot.49: 777-783.
  20. Li, R., Min, D., Chen, L., Chen, C. and Hu, X. (2017). Hydroprimimg accelerates seed germination of Medicago sativa under stressful conditions: A thermal and hydrotime model approach. Legume Res. 40(4): 741-747.
  21. Li, W., Liu,X.,Khan,M.A. and Yamaguchi,S. (2005). The effect of plant growth regulators, nitricoxide, nitrate, nitrite and light on the germination of dimorphic seed of Suaeda salsa under ksaline conditions. J. Pl. Res.118: 207-214.
  22. Mani, F., Bettaieb, T., Zheni, K., Doudech, N., Hannachi, C. (2012) Effect of hydrogen peroxide and thiourea on fluorescence and tuberization of potato (Solanum tuberosum(L.). J. Stress Physiol. Biochem. 8: 61–71.
  23. Mathur, N., Singh, J., Bohra, S., Bohra, A. andVyas, A. (2006). Improved productivity ofmung bean by application of thiourea under arid conditions. World J. Agric. Sci.2:185-187.
  24. Mitoi, E.N., Holobiuc, I., and Blindu, R. (2009). The effect of mannitol on antioxidative enzymes in vitro long termcultures of Dianthus tenuifolius and Dianthus spiculifolius. Rom. J. Biol. Plant Biol. 54: 25-30.
  25. Pandey, M., Srivastava, A. K., D’Souza, S. F. and Penna, S. (2013) Thiourea, a ROS scavenger, regulates source-to-sink relationship to enhance crop yield and oil content in Brassica juncea (L.). PLoS ONE 8(9): 73921. 
  26. Paparella, S., Araújo, S.S., Rossi, G., Wijayasinghe, M., Carbonera, D. andBalestrazzi, A. (2015). Seed priming: state of the art and new perspectives. Plant Cell Rep. 34:1281-1293.
  27. Sahu, M.P., Kumawat, S.M., Ramaswamy, N.K., D’Souza, S.F. and Singh, G. (2006). Sulphydryl bioregulator technology for increasing wheat productivity. Res. Bull. RAU-BARC 1–56.
  28. Singh, S., Bansal, M.L., Singh, T.P. and Kumar, R.(2001) Statistical Methods for Research Workers, Kalyani Publishers, New Delhi,.
  29. Singh, S. and Rathore,P.S. (2003). Influence of phosphorus and thiourea on yield and economics of greengram [Vigna radiata var. aureus (L.) Wilczek]. Res. on Crops 4(2): 210-212.
  30. Srivastava, A.K., Ramaswamy, N.K., Suprasanna, P. and D’Souza, S.F. (2010). Genome-wide analysis of thiourea-modulated salinity stress responsive transcripts in seeds of Brassica juncea: identification of signalling and effector components of stress tolerance. Ann.Bot.106:663-674.
  31. Tanou, G., Filippou, P. and Belghazi, M. (2012).Oxidative and nitrosative-based signalling and associated post-translational modifications orchestrate the acclimation of citrus plants to salinity stress. Plant J. 72:585–599.
  32. Yoshiyama, M., Maruyama,A., Atsumi, T. and Esashi,Y. (1996). Mechanism of action of C2H2 in promoting the germination of cocklebur seeds. III. A further enhancement of priming effect withnitrogenous compounds and C2H2 responsiveness of seeds. Aust. J. Pl. Physiol. 23:519-525.
  33. Zavariyan, A., Rad, M., Asghari, M. (2015). Effect of seed priming by potassium nitrate on germination andbiochemical indices in Silybum marianum L. under salinity stress. Int. J. Life Sci. 9: 23-29. 

Global Footprints