Legume Research-An International Journal

Genetic diversity analysis in underutilized medicinal climber Mucuna pruriens (L.) DC. germplasm revealed by inter simple sequence repeats markers

DOI: 10.18805/LR-3954    | Article Id: LR-3954 | Page : 32-37
Citation :- Genetic diversity analysis in underutilized medicinal climber Mucuna pruriens (L.) DC. germplasm revealed by inter simple sequence repeats markers.Legume Research-An International Journal.2020.(43):32-37
A. Chinapolaiah, K. Hima Bindu, G.N. Khadke, G.N. Manjesh, N. Hariprasad Rao, S. Sudheer Kumar and Manish Kumar Suthar acpolireddy.hortico@gmail.com
Address : ICAR-Indian Institute of Horticultural Research, Hessaraghatta, Bengaluru-560089, Karnataka, India.
Submitted Date : 12-10-2017
Accepted Date : 10-07-2018
First Online:

Abstract

Velvet bean is an important medicinal legume, its seeds are prominent source of L-Dopa. The present investigation on genetic diversity assessment of 58 germplasm of velvet bean by using 11 ISSR markers. Out of 63 amplified products 59 were showed polymorphism and 4 were monomorphic with an average of 5.7 bands amplified per primer. According to band statistics and efficiency parameters showed the primers UBC 827, UBC 834 and UBC 836 were more efficient. The highest genetic similarity values (0.90) were observed between IIHR MP 102 and IIHR MP 74-3. In dendrogram germplasm grouped into two major clusters at 63 per cent similarity. Among the germplasm, IIHR Selection 4, IIHR Selection 10, IIHR MP 9, IC 33243 and IIHR MP 7 were found to be distinctly divergent, can be used in the further breeding programme.

Keywords

Genetic diversity Germplasm ISSR markers L-Dopa Mucuna pruriens Polymorphism.

References

  1. Bishoyi, A.K., Pillai, V.V., Geetha, K.A. and Maiti, S. (2014). Assessment of genetic diversity in Clitoria ternata populations from different parts of India by RAPD and ISSR markers. Genetic Resources and Crop Evolution, 61:1597-1609.
  2. Capo-chichi, L.J.A., Weaver, D.B. and Morton, C.M. (2003) .The use of molecular markers to study genetic diversity in Mucuna. Tropical and Subtropical Agroecosystems, 1:309-318.
  3. Dellapotra, S.J., Wood, G. and Hicks, J.B. (1983). A plant mini preparation: version 2. Plant Molecular Biology Reproduction, 1:19-21.
  4. Kalidass, C. and Mohan, V.R. (2010). Genetic diversity of velvet bean, Mucuna pruriens (L.) DC from Southern Western Ghats, India. International Journal of Biology and Technology, 1(2):31-37.
  5. Kameli, M., Hejazi, S.M.H. and Ebadi, M. (2013). Assessment of genetic diversity on populations of three Satureja species in Iran using ISSR markers. Annals of Biological Research, 4 (3):64-72.
  6. Leelambika, M., Mahesh, S., Jaheer, M., Sathyanarayana, N. (2010). Comparative evaluation of genetic diversity among Indian Mucuna species using morphometric, biochemical and molecular approaches. World Journal of Agricultural Sciences, 6(5):568-578. 
  7. Manyam, B.V. (1995). An alternate medicine treatment for Parkinson’s disease results of a multicenter clinical trial-HP 200 in Parkinson’s disease study group. Journal of Alternative Component Medicine, 1:249-255.
  8. Mathur, P., Habibi, N., Chittora, M. and Purohit, S.D. (2012). Molecular analysis of variability among genotypes of Abrus precatorius L. with different seed coat colours using RAPD and ISSR markers. Indian Journal of Biotechnology, 12:273-276.
  9. Padmesh, P., Reji, J.V., Jinish Dhar, M. and Seeni, S. (2006). Estimation of genetic diversity in varieties of Mucuna pruriens using RAPD, Biologia Plantarum, 50(3):367-372.
  10. Powell, W., Morgante, M., Andre, C., Hanafey, M. and Vogel, J. (1996). The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for analysis. Molecular Breeding, 2:107-111.
  11. Prevost, A. and Wilkinson, M.J. (1999). A new system for comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theoretical and Applied Genetics, 98:107-112.
  12. Rohlf, F.J. (1998). NTSYS-pc Numerical taxonomy and multivariate analysis system, version 2.02. Exeter Publishing Ltd. Setauket, New York, USA.
  13. Roldan-Ruiz, I., Dendauw, J., Van, B.E., Depicker, A. and De, L.M. (2000). AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.). Molecular Breeding, 6:125-134.
  14. Saini, M., Singh, S., Hussain, Z. and Sikka, V.K. (2010). RAPD analysis in mungbean (Vigna radiata (L.) Wilczek.) ÉÉ: comparison of efficiency parameters of RAPD primers, Indian Journal of Biotechnology, 9:276-282.
  15. Sao, M.K., Nair, S.K., Verulkar, S.B., Saxena, R.R. and Nanda, H.C. (2015). Molecular profiling and genetic diversity of mungbean (Vigna radiata L.) genotypes using ISSR and SSR markers. Indian Journal of Agricultural Research, 48(1):373-376. 
  16. Satyanarayana, N., Vikas, P.B., Bharat Kumar, T.N. and Rajesha, R. (2010). RAPD markers for genetic characterization in Mucuna species. Indian Journal of Genetics, 70(3):296-298.
  17. Shilpha, J., Silambarasan, T., Pandian, S.K. and Ramesh, M. (2013). Assessment of genetic diversity in Solanum trilobatum L., and important medicinal plant from south India using RAPD and ISSR markers. Genetic Resources and Crop Evolution, 60:807-818. 
  18. Tonk, F.A., Sonmez, C. and Furan, A. (2011). Characterization of various Hypericum perforatum clones by hypericin and RAPD analysis. International Journal of Agriculture and Biology, 13:31-37.
  19. Vyas, D., Joshi, A., Rajamani, G., Jain, D. and Kaur, G. (2016). Genetic diversity analysis in different genotypes of black gram [Vigna mungo (L.) Hepper] using RAPD markers. Legume Research, 39(5):690-697. 
  20. Vyas, D., Joshi, A., Rajamani, G. and Jain, D. (2018). Assessment of genetic diversity in black gram [Vigna mungo (L.) Hepper] genotypes based on ISSR. Legume Research, 41(2):175-181.
  21. Weir, B.S. (1996). Genetic data analysis II: methods of discrete population genetic data, Sinauer Publishers, Sunderaland MA, USA. 

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