Legume Research

  • Chief EditorJ. S. Sandhu

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Legume Research, volume 43 issue 1 (february 2020) : 32-37

Genetic diversity analysis in underutilized medicinal climber Mucuna pruriens (L.) DC. germplasm revealed by inter simple sequence repeats markers

A. Chinapolaiah, K. Hima Bindu, G.N. Khadke, G.N. Manjesh, N. Hariprasad Rao, S. Sudheer Kumar, Manish Kumar Suthar
1ICAR-Indian Institute of Horticultural Research, Hessaraghatta, Bengaluru-560089, Karnataka, India.
  • Submitted12-10-2017|

  • Accepted10-07-2018|

  • First Online 15-11-2018|

  • doi 10.18805/LR-3954

Cite article:- Chinapolaiah A., Bindu Hima K., Khadke G.N., Manjesh G.N., Rao Hariprasad N., Kumar Sudheer S., Suthar Kumar Manish (2018). Genetic diversity analysis in underutilized medicinal climber Mucuna pruriens (L.) DC. germplasm revealed by inter simple sequence repeats markers. Legume Research. 43(1): 32-37. doi: 10.18805/LR-3954.
Velvet bean is an important medicinal legume, its seeds are prominent source of L-Dopa. The present investigation on genetic diversity assessment of 58 germplasm of velvet bean by using 11 ISSR markers. Out of 63 amplified products 59 were showed polymorphism and 4 were monomorphic with an average of 5.7 bands amplified per primer. According to band statistics and efficiency parameters showed the primers UBC 827, UBC 834 and UBC 836 were more efficient. The highest genetic similarity values (0.90) were observed between IIHR MP 102 and IIHR MP 74-3. In dendrogram germplasm grouped into two major clusters at 63 per cent similarity. Among the germplasm, IIHR Selection 4, IIHR Selection 10, IIHR MP 9, IC 33243 and IIHR MP 7 were found to be distinctly divergent, can be used in the further breeding programme.
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