ISSR based diversity analysis in beachpea [Vigna marina (Burm.) Merr.] accessions of Andaman and Nicobar Islands, India 

DOI: 10.18805/LR-3943    | Article Id: LR-3943 | Page : 38-42
Citation :- ISSR based diversity analysis in beachpea [Vigna marina (Burm.) Merr.] accessions of Andaman and Nicobar Islands, India.Legume Research-An International Journal.2020.(43):38-42
Awnindra K Singh, A. Velmurugan, Debjyoti Sen Gupta, N.P. Singh, S. Dam Roy, Utpal Biswas and R. Rahul Kumar awnindrakumar@gmail.com
Address : ICAR- Central Island Agricultural Research Institute, Port Blair-744 101, Andaman and Nicobar Islands, India.
Submitted Date : 14-09-2017
Accepted Date : 25-02-2019

Abstract

Beachpea [Vigna marina (Berm.) Merr.] is a less-known food legume species, usually found near to sandy sea beaches. In the present study, a total of 17 ISSR primers were used to study the genetic diversity of 16 accessions of beachpea. The number of amplicons of genomic DNA ranged from 4 (UBC 818) to 110 (HB 13) among accessions and varied in size from 125 bp to 3000 bp. Amplification of genomic DNA of 16 accessions produced 861 amplicons, of which 588 were polymorphic (68.29%) with an average of 35 polymorphic fragments per primer. Average PIC ranged from 0.01 (UBC 809) to 0.499 (UBC 811). UPGMA analysis grouped the accessions into two main clusters, I and II comprising of 12 and 4 accessions, respectively. The Jaccard similarity coefficient between different beachpea accessions ranged from 0.38 to 1.00. The higher degree of genetic variation in the accessions revealed by ISSR analysis could be useful in characterization and conservation point of view of wild Vigna species.

Keywords

Beachpea Genetic diversity ISSR markers Polymorphism Vigna marina

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