Legume Research

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Legume Research, volume 43 issue 1 (february 2020) : 38-42

ISSR based diversity analysis in beachpea [Vigna marina (Burm.) Merr.] accessions of Andaman and Nicobar Islands, India 

Awnindra K Singh, A. Velmurugan, Debjyoti Sen Gupta, N.P. Singh, S. Dam Roy, Utpal Biswas, R. Rahul Kumar
1ICAR- Central Island Agricultural Research Institute, Port Blair-744 101, Andaman and Nicobar Islands, India.
  • Submitted14-09-2017|

  • Accepted25-02-2019|

  • First Online 04-10-2019|

  • doi 10.18805/LR-3943

Cite article:- Singh K Awnindra, Velmurugan A., Gupta Sen Debjyoti, Singh N.P., Roy Dam S., Biswas Utpal, Kumar Rahul R. (2019). ISSR based diversity analysis in beachpea [Vigna marina (Burm.) Merr.] accessions of Andaman and Nicobar Islands, India. Legume Research. 43(1): 38-42. doi: 10.18805/LR-3943.
Beachpea [Vigna marina (Berm.) Merr.] is a less-known food legume species, usually found near to sandy sea beaches. In the present study, a total of 17 ISSR primers were used to study the genetic diversity of 16 accessions of beachpea. The number of amplicons of genomic DNA ranged from 4 (UBC 818) to 110 (HB 13) among accessions and varied in size from 125 bp to 3000 bp. Amplification of genomic DNA of 16 accessions produced 861 amplicons, of which 588 were polymorphic (68.29%) with an average of 35 polymorphic fragments per primer. Average PIC ranged from 0.01 (UBC 809) to 0.499 (UBC 811). UPGMA analysis grouped the accessions into two main clusters, I and II comprising of 12 and 4 accessions, respectively. The Jaccard similarity coefficient between different beachpea accessions ranged from 0.38 to 1.00. The higher degree of genetic variation in the accessions revealed by ISSR analysis could be useful in characterization and conservation point of view of wild Vigna species.
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