Biochemical changes in naturally aged seeds of soybean genotypes with good and poor storability
 

DOI: 10.18805/LR-3931    | Article Id: LR-3931 | Page : 782-788
Citation :- Biochemical changes in naturally aged seeds of soybean genotypes with good and poor storability.Legume Research-An International Journal.2019.(42):782-788
H.P. Vijayakumar, A. Vijayakumar, P. Srimathi, G. Somasundaram, S. Rajendra Prasad, S. Natarajan, R. Dhandapani, K.M. Boraiah and K. Vishwanath vijayhpm@yahoo.com
Address : Tamil Nadu Agricultural University, Coimbatore-641 003, Tamil Nadu
Submitted Date : 23-08-2017
Accepted Date : 18-08-2018

Abstract

Seed deterioration during storage is associated with various metabolic and chemical alterations that vary among genotypes in soybean. In the present study, five genotypes with good storability viz., kalitur, MACS 1416, EC 18761, CO1 and DSB21 and five genotypes with poor storability viz., JS 71-05, DS 228, MAUS 61, NRC 93 and DSB 24 were selected and evaluated for biochemical changes to identify the best donors for storability. Among the genotypes, all the poor storer genotypes showed faster accumulation of free amino acids, free fatty acids and malondialdehyde content as a result of oxidative stress. Poor storer genotypes also showed the increased lipoxygenase I & II enzymes activity. All the good storer genotypes showed higher anti-oxidative enzymes activity causing slower lipid peroxidation. Among the good storers, kalitur showed favourable biochemical features for storability while MAUS-61 was identified as poor storer exhibiting less favourable biochemical features.

Keywords

Biochemical changes Deterioration Genotypes Seed Soybean Storability.

References

  1. Abreu, L.A.D., Carvalho, M.L.M., Pinto, C.A.G., Kataoka, V.Y. and Silva, T.T.D. (2013). Deterioration of sunflower seeds during storage. Journal of Seed Science, 35(2): 240-247 
  2. Aebi, H. (1984). Catalase in vitro. Meth Enzymol., 105: 121-126.
  3. Anderson, J.D. and Baker, J.E. (1983). Deterioration of seeds during ageing. Phytopathology, 73: 321-325.
  4. Bailly, C., Bogatek-Leszczynska, R., Côme, D. and Corbineau, F. (2002). Changes in activities of antioxidant enzymes and lipoxygenase during growth of sunflower seedlings from seeds of different vigour. Seed Sci. Res., 12(1): 47-55.
  5. Baleševiæ-Tubiæ, M., Tatiæ, V., Ðorðeviæ, Z., Nikoliæ, J. and Subiæ Ðukiæ, V. (2011). Changes in soybean seeds as affected by accelerated and natural aging. Romanian Biotechnological Letters, 6(6): 6740–6747.
  6. Bellaloui N., Krishna, N.R., Bruns, H.A., Gillen, A.M., Mengistu, A., Zobiole, L.H.S., et al. (2011). Soybean seed composition and quality: Interactions of environment, genotype, and management practices. In: Soybeans: cultivation, uses and nutrition. [Maxwell JE (ed)] Nova Science Publishers, pp. 1–42. 
  7. Castillo, F.I., Penel, I. and Greppin, H. (1984). Peroxidase release induced by ozone in Sedum album leaves. Plant Physiol., 74: 846-851.
  8. Christiansen, M.K. and Moore, R.D. (1961). Temperature in viva hydrolysis of cotton seed oil. Crop Sci., 1: 285-286.
  9. Demirkaya, M., Dietz, K.J. and Sivritepe, H.O. (2010). Changes in antioxidant enzymes during ageing of onion seeds. Not. Bot. Hort. Agrobot. Cluj., 38 (1) : 49- 52.
  10. Dhindsa, R.A., Dhindsa, P.P. and Thorpe, T.A. (1981). Leaf senescence: correlated with increased permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J. Exp. Bot., 126: 93-101.
  11. El-Abady, M.I., El-Emam, A.A.M., Seadh, S.E. and Yousof, F.I. (2012). Soybean seed quality as affected by cultivars, threshing methods and storage periods. Research Journal of Seed Science, 5: 115–125.
  12. Gabriel, L., Bonelli, G. and Dianzani, M.U. (1977). Inhibition of colchicine binding to rat liver tubulin by aldehydes and by linoleic acid hydroperoxide. Chemical-Biological Interactions, 19: 101-109.
  13. Ghassemi-Golezani, K., Bakhshy, J., Raey, Y. and Hossainzadeh-Mahootchy, A. (2010). Seed vigor and field performance of winter oilseed rape (Brassica napus L.), cultivars. Not. Bot. Hort. Agrobot. Cluj., 38(3): 146-150.
  14. Goel, A., Goel, A.K and Sheoran, I.S. (2003). Changes in oxidative stress enzymes during artificial ageing in cotton (Gossypium hirsutum L.) seeds. Journal of Plant Physiology, 160 (9):1093-1100.
  15. Grossman, S and Zakut, R. (1979). Determination of the activity of lipoxygenase (lipoxidase). Methods Biochem Anal., 25: 303-329.
  16. Harrington, J.F. (1972). Seed storage and longevity. In: “Seed Biology” Vol. III. (Ed. T. T. Kozlowski) Academic Press, New York, pp: 145-245.
  17. Heath, R.L. and Parker, L. (1968). Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys., 125: 189- 198.
  18. Hosamani, J., Dadlani, M., Santha, I.M., Arun Kumar, M.B. and Jacob, S.R. (2013). Biochemical phenotyping of soybean [Glycine max (L.) Merill] genotypes to establish the role of lipid peroxidation and antioxidant enzymes in seed longevity. Agrl. Res.,2(2): 119–126.
  19. ISTA. (2007). International Rules for Seed Testing. International Seed Testing Association, Bassersdorf, Switzerland.
  20. Jain, N., Kooper, R. and Saxena, S. (2006). Effect of accelerated ageing on seeds of radish (Raphanus sativus L.). Asian J. Plant Sci., 5: 461-464.
  21. Kaewnaree, P., Vichitphan, S., Klanrit, P., Siri, B. and Vichitphan, K. (2011). Effects of accelerated ageing process on seed quality and biochemical changes in Sweet pepper (Capsicum annuum L.) seeds. Biotechnol., 10(2): 175-182.
  22. Kibinza S., Vinel, D., Côme, D., Bailly, C. and Corbineau, F. (2006). Sunflower seed deterioration as related to moisture content during ageing, energy metabolism and active oxygen species scavenging. Physiologia Plantarum, 128 (3): 496-506.
  23. Koleckar, V., Jun, D., Opletal, L., Jahodar, L. and Kuca, L. (2007). Assay of radical scavenging activity of antidotes against chemical warfare by DPPH test using sequential injection technique. J. Appl. Biomed., 5: 81-84. 
  24. Kuchlan, M. K. (2006). Identification of physical, physiological and biochemical factors and molecular analysis for longevity of soybean seeds. Ph.D. Thesis. Division of Seed Science and Technology, IARI, New Delhi.
  25. Misra, P.S., Mertz, E.T. and Glover, D.V. (1975). Free amino acid content estimation. Cereal Chem., 52: 844.
  26. Moore, S. and Stein, W.H. (1948). In: Methods in Enzymol. (Eds. Colowick, S.P. and N.D. Kaplan) Academic Press, New York, 3, p 468.
  27. Rahul K.C., Geetanjly, Zeba, K. and Radhamani, J. (2016). Changes in enzyme activity during accelerated ageing in soybean (Glycine max (L.) Merrill), Legume Genomics and Genetics, 7(9): 1-7.
  28. Sadasivam, S. and Manickam, A. (1995). Proteins and enzymes. In: Biochemical Methods for Agricultural Sciences, Wiley Eastern Ltd., New Delhi, pp. 33-143.
  29. Salam, M.A., Haque, M.M., Islam, M.O., Uddin, M.N. and Haque, M.N. (2018). Biochemical changes in seeds of five cotton genotypes stored in different packaging materials at ambient condition. Advances in Plants & Agriculture Research, 8 (2): 207-211.
  30. Scialabba, A., Bellani, L.M. and Dell’aquila, A. (2002). Effects of ageing on peroxidase activity and localization in radish (Raphanus sativus L.) seeds. European J. Histochemistry, 46 (4): 351–8.
  31. Shelar, V.R. (2002). Role of mechanical damage in deterioration of soybean seed quality during storage. Ph.D. Thesis, MPKV, Rahuri (MS).
  32. Shelar, V.R., Shaikh, R.S. and Nikam, A.S. (2008). Soybean seed quality during storage: A review. Agric. Rev., 29(2): 125-131.
  33. Simic, B., Popovic, R., Sudaric, A., Rozman, V., Kalinovic, I. and Cosic, J. (2007). Influence of storage condition on seed oil content of maize, soybean and sunflower. Agriculture Conspectus Scientifics, 72 (3): 211-213.
  34. Smith, I.K., Vierheller, T.L. and Thorne, C.A. (1988). Assay of glutathione reductase in crude tissue homogenates using 5, 5'-dithiobis (2-nitrobenzoic acid). Annal. Biochem., 175: 408-413.
  35. Vijayakumar, H. P and Vijayakumar, A. (2016). Screening of soybean varieties for seed storability using accelerated ageing test. International Journal of Agricultural Science and Research, 6 (1):93-98. 

Global Footprints