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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 42 issue 5 (october 2019) : 710-714,   Doi: 10.18805/LR-3925

Molecular tagging of botrytis grey mould disease in chickpea

R
Ranjana
A
Anju Arora
R
R.K. Panwar
S
S.K. Verma
1Department of Genetics and Plant Breeding, G.B. Pant University of Agriculture and Technology, Pantangar-263 145, Uttarakhand, India.

  • Submitted11-08-2017|

  • Accepted09-05-2018|

  • First Online 16-10-2018|

  • doi 10.18805/LR-3925

Cite article:- Ranjana, Arora Anju, Panwar R.K., Verma S.K. (2018). Molecular tagging of botrytis grey mould disease in chickpea. Legume Research. 42(5): 710-714. doi: 10.18805/LR-3925.

ABSTRACT

Validation of two primers TA144 and TR29 for their linkage with botrytis grey mould resistance gene was studied in twenty genotypes of chickpea including resistant (GL10006) and susceptible (DCP92-3) checks. The genotypes were also scored for disease reaction under natural exposure at hot spot location. Molecular analysis with primers TA144 and TR29 showed polymorphic bands in GL10006 and DCP92-3. Therefore, it can be concluded that the primers are linked with resistance gene. The genotypes that showed amplicons with both the primers at same or nearby positions of resistant check were HK-2, HK-4, BG-1003, KAK-2 and GNG-1969 and indicated as resistant or moderately resistant ones. The above genotypes were also found resistant under natural field condition. Thus both the primers TA144 and TR29 showed good correlation with phenotypic evaluation in most of the genotypes studied. In future, more number of primers nearby the resistance gene(s) can be tried for their contribution towards overall disease reaction.

REFERENCES

  1. Anbessa, Y., Taran, B., Warkentin, T.D., Tullu, A. and Vandenberg, A. (2009). Genetic analyses and conservation of QTL for ascochyta blight resistance in chickpea (Cicer arietinum L.). Theor. Appl. Genet. 119: 757-765.
  2. Chakraborthi, D., Sarkar, A. and Guptha, S. (2006). Small and large scale genomic DNA isolation protocol for chickpea (Cicer arietinum L.) suitable for molecular marker transgenic analysis. African J. Biotech. 5(8): 585-589.
  3. Chetukuri, A., Gaur, P. M., Pande, S., Gali, K. K.; Ganesh, M.,Kumar, J. and Varshney, R. K. (2011). Mapping QTL for resistance to botrytis grey mould in chickpea. Euphytica, 182: 1-9.
  4. Davidson, J. A., Pande, S., Bretag, T. W., Lindbeck, K. D. and Kishore, G. K. (2004). Biology and management of Botrytis spp. in legume crops. In: Botrytis biology, pathology and control. Kluwer Academic Publishers: The Netherlands. pp. 295–318.
  5. Grewal, J. S. and Laha, S. K. (1983). Chemical control of botrytis blight in chickpea. Indian Phytopathol, 36: 516–520.
  6. Kaur, L., Sirari, A., Kumar, D., Sandhu, J.S., Singh, S., Kapoor, K., Singh, I., Gowda, C.L.L., et al. (2013). Combining Ascochyta blight and Botrytis grey mould resistance in chickpea through interspecific hybridization. Phytopathologia Mediterranea. 52(1): 157-165.
  7. Knights, E. J. and Siddique, K. H.M. (2002). Manifestation of Botrytis cinerea on chickpeas in Australia. In: Workshop Proceedings Integrated Management of Botrytis Grey Mould of Chickpea in Bangladesh and Australia, Bangladesh Agricultural Research Institute: Joydebpur, Gazipur, Bangladesh. pp. 70-77.
  8. McIntosh, M. and Miller, C. (2001). A diet containing food rich in soluble and insoluble fiber improves glycemic control and reduces hyperlipidemia among patients with type 2 diabetes mellitus. Nutrition Reviews. 59: 52-5.
  9. Rashid, M.H., Shahadat, M.K., Hasnat, M.R. Kashem, M.A., Rafii, M.Y. and Latif, M.A. (2015). Screening of deshi chickpea (Cicer arietinum L.) germplasm resistant to Botrytis Gray Mold in Bangladesh. Legume Research. 38(6): 861-863. 
  10. Pande, S., Galloway, J., Gaur, P. M., Siddique, K. H. M., Tripathi, H. S., Taylor, P., MacLeod, M. W. J., et al . (2006). Botrytis grey mould of chickpea: a review of biology, epidemiology, and disease management. Australian Journal of Agricultural Research, 57: 1137–1150.
  11. Pande, S., Ramgopal, D., Kishore, G. K., Mallikarjuna, N., Sharma, M., Pathak, M. and Rao, N. J. et al. (2006). Evaluation of Wild Cicer Species for Resistance to Ascochyta Blight and Botrytis Gray Mold in Controlled Environment at ICRISAT, Patancheru, India. 2(1): 33-35.
  12. Pittaway, J.K. Ahuja, K.D., Cehun, M., Chronopoulos, A., Robertson, I.K., Nestel, P.J. and Ball, M.J. (2006). Dietary supplementation with chickpeas for at least 5 weeks results in small but significant reductions in serum total and low-density lipoprotein cholesterols in adult women and men. Annals of Nutrition and Metabolism. 50: 512-518. 
  13. Winter, P., Pfaff, T., Udupa, S.M., Huettel, B., Sharma, P. C., Sahi, S., Arrequin-Espinoza, R., et al. (1999). Characterization and mapping of sequence- tagged microsatellite sites in the chickpea (Cicer arietinum L.). Mol. Genet. 296: 90-101.
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    Research Article

    volume 42 issue 5 (october 2019) : 710-714,   Doi: 10.18805/LR-3925

    Molecular tagging of botrytis grey mould disease in chickpea

    R
    Ranjana
    A
    Anju Arora
    R
    R.K. Panwar
    S
    S.K. Verma
    1Department of Genetics and Plant Breeding, G.B. Pant University of Agriculture and Technology, Pantangar-263 145, Uttarakhand, India.

    • Submitted11-08-2017|

    • Accepted09-05-2018|

    • First Online 16-10-2018|

    • doi 10.18805/LR-3925

    Cite article:- Ranjana, Arora Anju, Panwar R.K., Verma S.K. (2018). Molecular tagging of botrytis grey mould disease in chickpea. Legume Research. 42(5): 710-714. doi: 10.18805/LR-3925.

    ABSTRACT

    Validation of two primers TA144 and TR29 for their linkage with botrytis grey mould resistance gene was studied in twenty genotypes of chickpea including resistant (GL10006) and susceptible (DCP92-3) checks. The genotypes were also scored for disease reaction under natural exposure at hot spot location. Molecular analysis with primers TA144 and TR29 showed polymorphic bands in GL10006 and DCP92-3. Therefore, it can be concluded that the primers are linked with resistance gene. The genotypes that showed amplicons with both the primers at same or nearby positions of resistant check were HK-2, HK-4, BG-1003, KAK-2 and GNG-1969 and indicated as resistant or moderately resistant ones. The above genotypes were also found resistant under natural field condition. Thus both the primers TA144 and TR29 showed good correlation with phenotypic evaluation in most of the genotypes studied. In future, more number of primers nearby the resistance gene(s) can be tried for their contribution towards overall disease reaction.

    REFERENCES

    1. Anbessa, Y., Taran, B., Warkentin, T.D., Tullu, A. and Vandenberg, A. (2009). Genetic analyses and conservation of QTL for ascochyta blight resistance in chickpea (Cicer arietinum L.). Theor. Appl. Genet. 119: 757-765.
    2. Chakraborthi, D., Sarkar, A. and Guptha, S. (2006). Small and large scale genomic DNA isolation protocol for chickpea (Cicer arietinum L.) suitable for molecular marker transgenic analysis. African J. Biotech. 5(8): 585-589.
    3. Chetukuri, A., Gaur, P. M., Pande, S., Gali, K. K.; Ganesh, M.,Kumar, J. and Varshney, R. K. (2011). Mapping QTL for resistance to botrytis grey mould in chickpea. Euphytica, 182: 1-9.
    4. Davidson, J. A., Pande, S., Bretag, T. W., Lindbeck, K. D. and Kishore, G. K. (2004). Biology and management of Botrytis spp. in legume crops. In: Botrytis biology, pathology and control. Kluwer Academic Publishers: The Netherlands. pp. 295–318.
    5. Grewal, J. S. and Laha, S. K. (1983). Chemical control of botrytis blight in chickpea. Indian Phytopathol, 36: 516–520.
    6. Kaur, L., Sirari, A., Kumar, D., Sandhu, J.S., Singh, S., Kapoor, K., Singh, I., Gowda, C.L.L., et al. (2013). Combining Ascochyta blight and Botrytis grey mould resistance in chickpea through interspecific hybridization. Phytopathologia Mediterranea. 52(1): 157-165.
    7. Knights, E. J. and Siddique, K. H.M. (2002). Manifestation of Botrytis cinerea on chickpeas in Australia. In: Workshop Proceedings Integrated Management of Botrytis Grey Mould of Chickpea in Bangladesh and Australia, Bangladesh Agricultural Research Institute: Joydebpur, Gazipur, Bangladesh. pp. 70-77.
    8. McIntosh, M. and Miller, C. (2001). A diet containing food rich in soluble and insoluble fiber improves glycemic control and reduces hyperlipidemia among patients with type 2 diabetes mellitus. Nutrition Reviews. 59: 52-5.
    9. Rashid, M.H., Shahadat, M.K., Hasnat, M.R. Kashem, M.A., Rafii, M.Y. and Latif, M.A. (2015). Screening of deshi chickpea (Cicer arietinum L.) germplasm resistant to Botrytis Gray Mold in Bangladesh. Legume Research. 38(6): 861-863. 
    10. Pande, S., Galloway, J., Gaur, P. M., Siddique, K. H. M., Tripathi, H. S., Taylor, P., MacLeod, M. W. J., et al . (2006). Botrytis grey mould of chickpea: a review of biology, epidemiology, and disease management. Australian Journal of Agricultural Research, 57: 1137–1150.
    11. Pande, S., Ramgopal, D., Kishore, G. K., Mallikarjuna, N., Sharma, M., Pathak, M. and Rao, N. J. et al. (2006). Evaluation of Wild Cicer Species for Resistance to Ascochyta Blight and Botrytis Gray Mold in Controlled Environment at ICRISAT, Patancheru, India. 2(1): 33-35.
    12. Pittaway, J.K. Ahuja, K.D., Cehun, M., Chronopoulos, A., Robertson, I.K., Nestel, P.J. and Ball, M.J. (2006). Dietary supplementation with chickpeas for at least 5 weeks results in small but significant reductions in serum total and low-density lipoprotein cholesterols in adult women and men. Annals of Nutrition and Metabolism. 50: 512-518. 
    13. Winter, P., Pfaff, T., Udupa, S.M., Huettel, B., Sharma, P. C., Sahi, S., Arrequin-Espinoza, R., et al. (1999). Characterization and mapping of sequence- tagged microsatellite sites in the chickpea (Cicer arietinum L.). Mol. Genet. 296: 90-101.
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