Banner
Current IssueEditorial BoardOnline First ArticlesArchive
Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 42 issue 4 (august 2019) : 447-453,   Doi: 10.18805/LR-3907

Comparative analysis of diversity based on morpho-metric and molecular markers in chickpea over different environments 

I
Indu Rialch
R
Rama Kalia
H
H.K. Chaudhary
B
B. Kumar
J
J.C. Bhandari
V
V. K. Sood
1Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, Himachal Pradesh, India.

  • Submitted28-06-2017|

  • Accepted22-02-2018|

  • First Online 20-06-2018|

  • doi 10.18805/LR-3907

Cite article:- Rialch Indu, Kalia Rama, Chaudhary H.K., Kumar B., Bhandari J.C., Sood K. V. (2018). Comparative analysis of diversity based on morpho-metric and molecular markers in chickpea over different environments. Legume Research. 42(4): 447-453. doi: 10.18805/LR-3907.

ABSTRACT

Ten morpho-agronomic traits and 80 random amplified polymorphic DNA (RAPD) molecular markers were used to survey genetic diversity in 25 chickpea genotypes. Analysis of variance revealed significant variability among different genotypes for morpho-metric traits. The cluster analysis done using morpho-metric traits grouped 25 genotypes into seven and six clusters in Environment I (Env. I) and Environment II (Env. II), respectively. Three genotypes viz., ICCV-96904, HPG-17, ICCV-95503 and L-HR-1 belonging to diverse clusters were identified divergent and may use in heterosis breeding programme. Of 80 random RAPD markers, 25 were found polymorphic. Three major clusters were identified using 25 polymorphic RAPD markers. The genetic similarity coefficient among genotypes ranged from 0.57 to 0.91. The average polymorphic information content (PIC) for 25 RAPD markers ranges from 0.12 to 0.40. D2-statistic, RAPD analysis and study of genotypes performance revealed sufficient genetic diversity among chickpea genotypes which would be useful in future breeding programme. 

REFERENCES

  1. Ahmad, F., Khan, A. I., Awan, F. S., Sadia, B., Sadaqat, H. A. and Bahadur, S. (2010).Genetic diversity of chickpea (Cicer arietinum L.) germplasm in Pakistan as revealed by RAPD analysis. Genetics and Molecular Research,9: 1414-1420.
  2. Babbar, A., Prakash, V., Tiwari, P. and Iquebal M. A. (2012). Genetic variability for chickpea (Cicer arietinum L.) under late sown season. Legume Research,35: 1-7.
  3. Bharadwaj, C., Srivastava, R., Chauhan, S. K., Satyavathi, C. T., Kumar, J., Faruqui, A., Yadav, S., Rizvi, A. H. and Kumar, T. (2011). Molecular diversity and phylogeny in geographical collection of chickpea (Cicer sp.) accessions. Journal of Genetics,90: 94-100.
  4. Doyle, J. J. and Doyle, D. L. (1987). Isolation of plant DNA from fresh tissue. Phytochem Bull 19: 11-15.
  5. Durga, K. K., Rao, Y. K. and Reddy, M. V. (2005). Genetic divergence in chickpea (Cicer arietinum L.). Legume Research,28: 250-    255.
  6. Jayalakshmi, V., Kiran, R. C., Jyothirmayi, G., Trivikrama, R. A (2016). Studies on genetic diversity in chickpea utilizing morphological and total seed protein markers. Legume Research, 39: 323-325.
  7. Kaur, S., Gupta, A. K. and Kaur, N. (2013). Genetic diversity in chickpea (Cicer arietinum L.) ultivars using RAPD Markers. Indian Journal of Agricultural Biochemistry, 26: 10-17.
  8. Mahalanobis, P. C. (1936). On the generalized distance in statistics. In: Proceedings National Academy of Sciences,India 2: 49-55.
  9. Mohammad, N., Ghafoor, A., Khan, M.R., Ahmad, H., Qureshi, A.S. and Ali, H. (2007). Genetic diversity and geographic relationship among local and exotic chickpea germplasm. Pakistan Journal of Botany, 39: 1575-1581
  10. Parashi, V. S., Lad, D. B., Mahse, L. B., Kute, N. S. and Sonawane, C. J. (2013). Genetic diversity studies in chickpea (Cicer arietinum L.). Journal of Life Sciences,10: 337-341.
  11. Rao, C. R. (1952). Advance Statistical Methods in biometrical Research. John Wiley and Sons Inc. New York. 1.
  12. Rao, L. S., Rani, P. U., Deshmukh, P. S., Kumar, P. A. and Panguluri, S. K. (2007). RAPD and ISSR fingerprinting in cultivated chickpea (Cicer arietinum L.) and its wild progenitor Cicer reticulatum Ladizinsky. Genetic Resources and Crop Evolution, 54: 1235-1244.
  13. Rohlf, F. J. (1993). NTSYS-pc: Numerical Taxonomic System of Multivariate Analysis System, Version 1.80. Exeter Software: Setauket, New York.
  14. Rolden- Ruiz I., Dendauw, J., Van Bockstaele, E., Depicker, A., De Loose, M. (2000): AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.). Molecular Breeding, 6: 125-134
  15. Upadhaya, H. D., Dwivedi, S. L., Gowda, C. L. L. and Singh, S. (2007). Identification of diverse germplasm lines for agronomic traits in chickpea (Cicer arietinum L.) core collection for use in crop improvement. Field Crops Research,100: 320-326.
  16. Van der Maesen, L. J. G. (1987). Origin, history and taxonomy of chickpea. In: Saxena MC, Singh KB (eds) The chickpea. C.A.B., Wallingford, pp 11–34.
  17. Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafalsky, J. A. and Tingey, S. V. (1990). DNA polymorphism amplified by arbitary primers are useful as genetic markers. Nucleic Acid Research,18: 6531–6535. 
Published In
Legume Research
Article Metrics
Metrics Icon
0
Views
0
Citations
Reviewed By
Share Article
Download Article
In this Article
    Contact us
    Follow us

    Research Article

    volume 42 issue 4 (august 2019) : 447-453,   Doi: 10.18805/LR-3907

    Comparative analysis of diversity based on morpho-metric and molecular markers in chickpea over different environments 

    I
    Indu Rialch
    R
    Rama Kalia
    H
    H.K. Chaudhary
    B
    B. Kumar
    J
    J.C. Bhandari
    V
    V. K. Sood
    1Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, Himachal Pradesh, India.

    • Submitted28-06-2017|

    • Accepted22-02-2018|

    • First Online 20-06-2018|

    • doi 10.18805/LR-3907

    Cite article:- Rialch Indu, Kalia Rama, Chaudhary H.K., Kumar B., Bhandari J.C., Sood K. V. (2018). Comparative analysis of diversity based on morpho-metric and molecular markers in chickpea over different environments. Legume Research. 42(4): 447-453. doi: 10.18805/LR-3907.

    ABSTRACT

    Ten morpho-agronomic traits and 80 random amplified polymorphic DNA (RAPD) molecular markers were used to survey genetic diversity in 25 chickpea genotypes. Analysis of variance revealed significant variability among different genotypes for morpho-metric traits. The cluster analysis done using morpho-metric traits grouped 25 genotypes into seven and six clusters in Environment I (Env. I) and Environment II (Env. II), respectively. Three genotypes viz., ICCV-96904, HPG-17, ICCV-95503 and L-HR-1 belonging to diverse clusters were identified divergent and may use in heterosis breeding programme. Of 80 random RAPD markers, 25 were found polymorphic. Three major clusters were identified using 25 polymorphic RAPD markers. The genetic similarity coefficient among genotypes ranged from 0.57 to 0.91. The average polymorphic information content (PIC) for 25 RAPD markers ranges from 0.12 to 0.40. D2-statistic, RAPD analysis and study of genotypes performance revealed sufficient genetic diversity among chickpea genotypes which would be useful in future breeding programme. 

    REFERENCES

    1. Ahmad, F., Khan, A. I., Awan, F. S., Sadia, B., Sadaqat, H. A. and Bahadur, S. (2010).Genetic diversity of chickpea (Cicer arietinum L.) germplasm in Pakistan as revealed by RAPD analysis. Genetics and Molecular Research,9: 1414-1420.
    2. Babbar, A., Prakash, V., Tiwari, P. and Iquebal M. A. (2012). Genetic variability for chickpea (Cicer arietinum L.) under late sown season. Legume Research,35: 1-7.
    3. Bharadwaj, C., Srivastava, R., Chauhan, S. K., Satyavathi, C. T., Kumar, J., Faruqui, A., Yadav, S., Rizvi, A. H. and Kumar, T. (2011). Molecular diversity and phylogeny in geographical collection of chickpea (Cicer sp.) accessions. Journal of Genetics,90: 94-100.
    4. Doyle, J. J. and Doyle, D. L. (1987). Isolation of plant DNA from fresh tissue. Phytochem Bull 19: 11-15.
    5. Durga, K. K., Rao, Y. K. and Reddy, M. V. (2005). Genetic divergence in chickpea (Cicer arietinum L.). Legume Research,28: 250-    255.
    6. Jayalakshmi, V., Kiran, R. C., Jyothirmayi, G., Trivikrama, R. A (2016). Studies on genetic diversity in chickpea utilizing morphological and total seed protein markers. Legume Research, 39: 323-325.
    7. Kaur, S., Gupta, A. K. and Kaur, N. (2013). Genetic diversity in chickpea (Cicer arietinum L.) ultivars using RAPD Markers. Indian Journal of Agricultural Biochemistry, 26: 10-17.
    8. Mahalanobis, P. C. (1936). On the generalized distance in statistics. In: Proceedings National Academy of Sciences,India 2: 49-55.
    9. Mohammad, N., Ghafoor, A., Khan, M.R., Ahmad, H., Qureshi, A.S. and Ali, H. (2007). Genetic diversity and geographic relationship among local and exotic chickpea germplasm. Pakistan Journal of Botany, 39: 1575-1581
    10. Parashi, V. S., Lad, D. B., Mahse, L. B., Kute, N. S. and Sonawane, C. J. (2013). Genetic diversity studies in chickpea (Cicer arietinum L.). Journal of Life Sciences,10: 337-341.
    11. Rao, C. R. (1952). Advance Statistical Methods in biometrical Research. John Wiley and Sons Inc. New York. 1.
    12. Rao, L. S., Rani, P. U., Deshmukh, P. S., Kumar, P. A. and Panguluri, S. K. (2007). RAPD and ISSR fingerprinting in cultivated chickpea (Cicer arietinum L.) and its wild progenitor Cicer reticulatum Ladizinsky. Genetic Resources and Crop Evolution, 54: 1235-1244.
    13. Rohlf, F. J. (1993). NTSYS-pc: Numerical Taxonomic System of Multivariate Analysis System, Version 1.80. Exeter Software: Setauket, New York.
    14. Rolden- Ruiz I., Dendauw, J., Van Bockstaele, E., Depicker, A., De Loose, M. (2000): AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.). Molecular Breeding, 6: 125-134
    15. Upadhaya, H. D., Dwivedi, S. L., Gowda, C. L. L. and Singh, S. (2007). Identification of diverse germplasm lines for agronomic traits in chickpea (Cicer arietinum L.) core collection for use in crop improvement. Field Crops Research,100: 320-326.
    16. Van der Maesen, L. J. G. (1987). Origin, history and taxonomy of chickpea. In: Saxena MC, Singh KB (eds) The chickpea. C.A.B., Wallingford, pp 11–34.
    17. Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafalsky, J. A. and Tingey, S. V. (1990). DNA polymorphism amplified by arbitary primers are useful as genetic markers. Nucleic Acid Research,18: 6531–6535. 
    In this Article
      Contact us
      Follow us
      Published In
      Legume Research

      Editorial Board

      View all (0)