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Research Article

volume 41 issue 2 (april 2018) : 222-225,   Doi: 10.18805/LR-387

SNP marker-based genetic mapping of rust resistance gene in the vegetable cowpea landrace ZN016

X
Xinyi Wu
B
Baogen Wang
X
Xiaohua Wu
Z
Zhongfu Lu
G
Guojing Li
P
Pei Xu
1Institute of Vegetables, State Key Lab. Breeding Base for Sustainable Control of Plant Pest and Disease, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310 021, People’s Republic of China.

  • Submitted22-09-2017|

  • Accepted31-10-2017|

  • First Online 29-12-2017|

  • doi 10.18805/LR-387

Cite article:- Wu Xinyi, Wang Baogen, Wu Xiaohua, Lu Zhongfu, Li Guojing, Xu Pei (2017). SNP marker-based genetic mapping of rust resistance gene in the vegetable cowpea landrace ZN016. Legume Research. 41(2): 222-225. doi: 10.18805/LR-387.

ABSTRACT

Cowpea rust, caused by Uromyces vignae, is one of the most destructive foliar diseases in cowpea [V. unguiculata. (L.) Walp]. Deployment of rust-resistant cultivars is the most environment-friendly and effective way to curb this disease. For this purpose, a fundamental step is to map the rust resistance genes in elite germplasm lines. In the current study, one major and two minor QTLs conferring rust resistance were detected in a 119-line recombinant inbred line (RIL) population derived from a cross between the rust resistant line “ZN016” and a susceptible cultivar “Zhijiang 282”, using the state-of-the-art cowpea iSelect Consortium Array.The major QTL, designated as Ruv1, was mapped to a 12.48 cM interval between the SNP markers 2_01772 and 2_03292 on LG09, which explained 34.8% of the phenotypic variation. The minor QTLs, designated as Ruv2 and Ruv3, were mapped to a 7.01 cM interval on LG7 and a 6.19 cM interval on LG8, which accounted for 13.4% and 11.9% of the phenotypic variation, respectively. This study demonstrates the usefulness of the Consortium SNP Array on complex trait mapping and solidifies the basis for marker-assisted breeding of new cowpea varieties with improved rust resistance.

REFERENCES

  1. Chandrashekar, M., Anil kumar, T.B. and Sailulla, M. (1989). Effect of different dates of sowing of cowpea on the severity of leaf rust caused by Uromyces phaseoli var vignae. Tropical Agric. 65: 149
  2. Leal-Bertioli, S.C.M., Cavalcante, U., Gouvea, E.G., Ballén-Taborda, C., Shirasawa, K., et al. (2015). Identification of QTLs for rust resistance in the peanut wild species arachis magna and the Development of KASP Markers for Marker-Assisted Selection. G3 Genes Genomes Genetics. 5: 1403-1413
  3. Li, G.J., Liu, Y.H., Ehlers, J.D., Zhu, Z.J., Wu, X.H., Wang, B.G., Lu, Z.F. (2007). Identification of an AFLP fragment linked to rust resistance in Asparagus Bean and its conversion to a SCAR marker. HortScience. 42(5): 1153-1156
  4. Michelmore, R.W., Paran, I., Kesseli, R.V. (1991). Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc. Natl. Acad. Sci. USA 88: 9828-9832
  5. Muñoz-Amatriaín, M., Mirebrahim, H., Xu, P., Wanamaker, S.I., Luo, M., et al. (2017). Genome resources for climate-resilient cowpea, an essential crop for food security. Plant J. 89: 1042–1054
  6. Rangaiah, S. (1997). Inheritance of resistance to Uromyces phaseolus in Vigna unguiculata (L.) Walp. Crop imrovement. 24: 251-252
  7. Singh, S.R. and Allen, D.J. (1979). In: Cowpea Pest and Diseases. International Institute of Tropical Agriculture, Ibadan, Nigeria
  8. Uma, M.S., Hegde, N.and Hittalmani, S. (2016). Identification of SSR marker associated with rust resistance in cowpea (Vigna unguiculata L.) using bulk segregant analysis. Legume Research. 39(1): 39-42
  9. Uma, M.S. and Salimath, P.M. (2004). Evaluation of segregating populations of cowpea for slow rusting. J.Arid Legumes. 1: 181-182
  10. Vuong, T.D., Walker, D.R., Nguyen, B.T., Nguyen, T.T., Dinh, H.X., Hyten, D.L., et al. (2016). Molecular Characterization of Resistance to Soybean Rust (Phakopsora pachyrhizi Syd. & Syd.) in Soybean Cultivar DT 2000 (PI 635999). PLOS ONE. DOI:10.1371/    journal.pone.0164493
  11. Wang, Y.F. (2004).In: Cowpea. China Agricultural Science and Technology Press, Beijing (in Chinese), p. 53–60
  12. Xu, P., Wu, X.Y., Muñoz-Amatriaín, M., Wang, B.G., Wu, X.H., Hu, Y.W., Huynh, B-L., et al. (2017). Genomic regions, cellular components and gene regulatory basis underlying pod length variations in cowpea (V. unguiculata L. Walp). Plant Biotechnol J. 15: 547–557
  13. Zhang,Y.R., Fan, M.R. and Huang, J.K. (1997). Study on inheritance of resistance to rust in yardlong bean. China Veg. 6:10–12 (in Chinese)
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    Research Article

    volume 41 issue 2 (april 2018) : 222-225,   Doi: 10.18805/LR-387

    SNP marker-based genetic mapping of rust resistance gene in the vegetable cowpea landrace ZN016

    X
    Xinyi Wu
    B
    Baogen Wang
    X
    Xiaohua Wu
    Z
    Zhongfu Lu
    G
    Guojing Li
    P
    Pei Xu
    1Institute of Vegetables, State Key Lab. Breeding Base for Sustainable Control of Plant Pest and Disease, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310 021, People’s Republic of China.

    • Submitted22-09-2017|

    • Accepted31-10-2017|

    • First Online 29-12-2017|

    • doi 10.18805/LR-387

    Cite article:- Wu Xinyi, Wang Baogen, Wu Xiaohua, Lu Zhongfu, Li Guojing, Xu Pei (2017). SNP marker-based genetic mapping of rust resistance gene in the vegetable cowpea landrace ZN016. Legume Research. 41(2): 222-225. doi: 10.18805/LR-387.

    ABSTRACT

    Cowpea rust, caused by Uromyces vignae, is one of the most destructive foliar diseases in cowpea [V. unguiculata. (L.) Walp]. Deployment of rust-resistant cultivars is the most environment-friendly and effective way to curb this disease. For this purpose, a fundamental step is to map the rust resistance genes in elite germplasm lines. In the current study, one major and two minor QTLs conferring rust resistance were detected in a 119-line recombinant inbred line (RIL) population derived from a cross between the rust resistant line “ZN016” and a susceptible cultivar “Zhijiang 282”, using the state-of-the-art cowpea iSelect Consortium Array.The major QTL, designated as Ruv1, was mapped to a 12.48 cM interval between the SNP markers 2_01772 and 2_03292 on LG09, which explained 34.8% of the phenotypic variation. The minor QTLs, designated as Ruv2 and Ruv3, were mapped to a 7.01 cM interval on LG7 and a 6.19 cM interval on LG8, which accounted for 13.4% and 11.9% of the phenotypic variation, respectively. This study demonstrates the usefulness of the Consortium SNP Array on complex trait mapping and solidifies the basis for marker-assisted breeding of new cowpea varieties with improved rust resistance.

    REFERENCES

    1. Chandrashekar, M., Anil kumar, T.B. and Sailulla, M. (1989). Effect of different dates of sowing of cowpea on the severity of leaf rust caused by Uromyces phaseoli var vignae. Tropical Agric. 65: 149
    2. Leal-Bertioli, S.C.M., Cavalcante, U., Gouvea, E.G., Ballén-Taborda, C., Shirasawa, K., et al. (2015). Identification of QTLs for rust resistance in the peanut wild species arachis magna and the Development of KASP Markers for Marker-Assisted Selection. G3 Genes Genomes Genetics. 5: 1403-1413
    3. Li, G.J., Liu, Y.H., Ehlers, J.D., Zhu, Z.J., Wu, X.H., Wang, B.G., Lu, Z.F. (2007). Identification of an AFLP fragment linked to rust resistance in Asparagus Bean and its conversion to a SCAR marker. HortScience. 42(5): 1153-1156
    4. Michelmore, R.W., Paran, I., Kesseli, R.V. (1991). Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc. Natl. Acad. Sci. USA 88: 9828-9832
    5. Muñoz-Amatriaín, M., Mirebrahim, H., Xu, P., Wanamaker, S.I., Luo, M., et al. (2017). Genome resources for climate-resilient cowpea, an essential crop for food security. Plant J. 89: 1042–1054
    6. Rangaiah, S. (1997). Inheritance of resistance to Uromyces phaseolus in Vigna unguiculata (L.) Walp. Crop imrovement. 24: 251-252
    7. Singh, S.R. and Allen, D.J. (1979). In: Cowpea Pest and Diseases. International Institute of Tropical Agriculture, Ibadan, Nigeria
    8. Uma, M.S., Hegde, N.and Hittalmani, S. (2016). Identification of SSR marker associated with rust resistance in cowpea (Vigna unguiculata L.) using bulk segregant analysis. Legume Research. 39(1): 39-42
    9. Uma, M.S. and Salimath, P.M. (2004). Evaluation of segregating populations of cowpea for slow rusting. J.Arid Legumes. 1: 181-182
    10. Vuong, T.D., Walker, D.R., Nguyen, B.T., Nguyen, T.T., Dinh, H.X., Hyten, D.L., et al. (2016). Molecular Characterization of Resistance to Soybean Rust (Phakopsora pachyrhizi Syd. & Syd.) in Soybean Cultivar DT 2000 (PI 635999). PLOS ONE. DOI:10.1371/    journal.pone.0164493
    11. Wang, Y.F. (2004).In: Cowpea. China Agricultural Science and Technology Press, Beijing (in Chinese), p. 53–60
    12. Xu, P., Wu, X.Y., Muñoz-Amatriaín, M., Wang, B.G., Wu, X.H., Hu, Y.W., Huynh, B-L., et al. (2017). Genomic regions, cellular components and gene regulatory basis underlying pod length variations in cowpea (V. unguiculata L. Walp). Plant Biotechnol J. 15: 547–557
    13. Zhang,Y.R., Fan, M.R. and Huang, J.K. (1997). Study on inheritance of resistance to rust in yardlong bean. China Veg. 6:10–12 (in Chinese)
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