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Legume Research

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Research Article

Legume Research, volume 41 issue 2 (april 2018) : 222-225

SNP marker-based genetic mapping of rust resistance gene in the vegetable cowpea landrace ZN016

Xinyi Wu, Baogen Wang, Xiaohua Wu, Zhongfu Lu, Guojing Li, Pei Xu
1Institute of Vegetables, State Key Lab. Breeding Base for Sustainable Control of Plant Pest and Disease, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310 021, People’s Republic of China.

  • Submitted22-09-2017|

  • Accepted31-10-2017|

  • First Online 29-12-2017|

  • doi 10.18805/LR-387

Cite article:- Wu Xinyi, Wang Baogen, Wu Xiaohua, Lu Zhongfu, Li Guojing, Xu Pei (2017). SNP marker-based genetic mapping of rust resistance gene in the vegetable cowpea landrace ZN016. Legume Research. 41(2): 222-225. doi: 10.18805/LR-387.

ABSTRACT

Cowpea rust, caused by Uromyces vignae, is one of the most destructive foliar diseases in cowpea [V. unguiculata. (L.) Walp]. Deployment of rust-resistant cultivars is the most environment-friendly and effective way to curb this disease. For this purpose, a fundamental step is to map the rust resistance genes in elite germplasm lines. In the current study, one major and two minor QTLs conferring rust resistance were detected in a 119-line recombinant inbred line (RIL) population derived from a cross between the rust resistant line “ZN016” and a susceptible cultivar “Zhijiang 282”, using the state-of-the-art cowpea iSelect Consortium Array.The major QTL, designated as Ruv1, was mapped to a 12.48 cM interval between the SNP markers 2_01772 and 2_03292 on LG09, which explained 34.8% of the phenotypic variation. The minor QTLs, designated as Ruv2 and Ruv3, were mapped to a 7.01 cM interval on LG7 and a 6.19 cM interval on LG8, which accounted for 13.4% and 11.9% of the phenotypic variation, respectively. This study demonstrates the usefulness of the Consortium SNP Array on complex trait mapping and solidifies the basis for marker-assisted breeding of new cowpea varieties with improved rust resistance.

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