Legume Research

  • Chief EditorJ. S. Sandhu

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Legume Research, volume 41 issue 2 (april 2018) : 303-310

Effect of Rhizobium inoculation to nodulation and growth of soybean [Glycine max (L.) Merrill] Germplasm 

Sakka Samudin, Heru Kuswantoro
1Indonesian Legume and Tuber Crops Research Institute, Indonesian Agency for Agricultural Research and Development. Jl. Raya Kendalpayak Km. 8 Malang – Indonesia 65101.
  • Submitted04-09-2017|

  • Accepted09-11-2017|

  • First Online 23-01-2018|

  • doi 10.18805/LR-385

Cite article:- Samudin Sakka, Kuswantoro Heru (2017). Effect of Rhizobium inoculation to nodulation and growth of soybean [Glycine max (L.) Merrill] Germplasm. Legume Research. 41(2): 303-310. doi: 10.18805/LR-385.
Nitrogen is an important macronutrient mineral that is needed in the largest amount by the plant and is the main limiting factor for plant development. Rhizobium is a group of bacteria capable of providing nutrients for soybean crops. When symbiotic with legume crops, this group of bacteria is able to infect plant roots and form root nodules. The study aimed to investigate the effect of Rhizobium on nodulation and growth of some soybean germplasm. The results showed no interaction between the inoculation treatment and the genotype. The significant effect of inoculation was shown by the number of nodules, nodule dry weight, root length, and root dry weight. Inoculation did not affect plant height and shoot dry weight. These traits were more influenced by genetic factors. In the condition without inoculation, the root length was higher but the root dry weight was lower. Plant height differed between the genotypes. KPT5 and KPT6 showed the highest plant height than any other genotypes. Both genotypes also had the highest shoot dry weight. The highest shoot dry weight was also achieved by KPT4.
  1. Abbasi, M.K, Majeed, A., Sadiq, A., and Khan, S.R. (2008). Application of Bradyrhizobium japonicum and phosphorus fertilization improved growth, yield and nodulation of soybean in the sub-humid hilly region of Azad Jammu and Kashmir, Pakistan. Plant Production Science, 11(3): 368-376.
  2. Abbasi, M.K., Manzoor, M., and Tahir, M.M. (2010). Efficiency of Rhizobium inoculation and P fertilization in enhancing nodulation, seed yield, and phosphorus use efficiency by field grown soybean under hilly region of Rawalakot Azad Jammu and Kashmir, Pakistan. Journal of Plant Nutrition, 33(7):1080-1102
  3. Aminu S. M., Shamsuddeen, U., and Dianda, M. (2015). Effects of inoculation on the growth of soybeans [Glycine max (L.) Merrill] planted in soils from different geographical location in North Western Nigeria. International Journal of Advances in Science Engineering and Technology, 3(3):38-42.
  4. Baraskar, V.V., Kachhadia, V.H., Vachhan, J.H., Barad, H.R., Patel, M.B., and Darwankar, M.S. (2014). Genetic variability, heritability and genetic advance in soybean [Glycine max (L.) Merrill]. Electronic Journal of Plant Breeding 5(4):802-806.
  5. Flavio, H., Boem, G., Javier, D., Rimski-Korsakov, H., and Raúl, S.L. (2004). Late season nitrogen fertilization of soybeans: effects on leaf senescence, yield and environment. Nutrient Cycling in Agro-Ecosystems. 68(2): 109-115.
  6. Furseth, B.J., Conley, S.P., and Ané, J.M. (2012). Soybean response to rhizobia and seed applied rhizobia inoculants in Wisconsin. Crop Science, 52(1): 339-344.
  7. Gaby, J.C., and Buckley, D.H. (2011). A global census of nitrogenase diversity. Environmental Microbiology, 13(7): 1790-1799.
  8. Golparvar, P., Mirshekari, B., and Borhani, P. (2012). Nitrogen spraying of soybeans at earlier flowering stage will be an ecological friendly fertilization management and improve crop yield. World Applied Sciences Journal 19(10): 1388-1392
  9. Hungria, M., Nogueira, M.A., and Araujo, R.S. (2015). Soybean Seed Co-Inoculation with Bradyrhizobium spp. and Azospirillum brasilense: A New Biotechnological Tool to Improve Yield and Sustainability. American Journal of Plant Sciences, 6(6):811-817.
  10. Hwang, S., Jeffery, D.R., Cregan, P.B., King, C.A., Davies, M.K., Purcell, L.C. (2014). Genetics and mapping of quantitative traits for nodule number, weight, and size in soybean (Glycine max L.[Merr.]). Euphytica, 195(3):419-434.
  11. King, C.A., Purcell, L.C., Bolton, A., and Specht, J.E. (2014). A possible relationship between shoot n concentration and the sensitivity of N2 fixation to drought in soybean. Crop Sci. 54(2): 746-756.
  12. Kuswantoro, H. (2017a). Genetic variability and heritability of acid-adaptive soybean promising lines. Biodiversitas 18(1): 378-382.
  13. Kuswantoro, H. (2017b). The role of heritability and genetic variability in estimated selection response of soybean lines on tidal swamp land. Pertanika Journal of Tropical Agricultural Science, 40(2): 319-328.
  14. López-Bucio, J., Cruz-Ramýìrez, A., and Herrera-Estrella, L. (2003). The role of nutrient availability in regulating root architecture. Current Opinion in Plant Biology, 6(3): 280-287.
  15. Manshuri, A.G. (2010). Pemupukan N, P, dan K pada kedelai sesuai kebutuhan tanaman dan daya dukung lahan. Penelitian Pertanian Tanaman Pangan, 29(3):171-179.
  16. Mohammadi, K., and Sohrabi, Y. (2012). Bacterial biofertilizers for sustainable crop production: a review. Journal of Agricultural and Biological Science, 7(5):307-316.
  17. Moreira, A., Moraes, L.A.C., Schroth, G., and Mandarino. J.M.G. (2015). Effect of nitrogen, row spacing, and plant density on yield, yield components, and plant physiology in soybean–wheat intercropping. Agronomy Journal, 107(6): 2162-2170.
  18. Schweiger, P., Hofer, M., Vollman, J., Wanek, W. (2014). The relationship between N isotopic fractionation within soybean and N2 fixation during soybean development. Physiolgia Plantarum, 152(3): 546-557.
  19. Sheirdil, R.A., Bashir, K., Hayat, R ., and Akhtar, M.S. (2012). Effect of cadmium on soybean (Glycine max L) growth and nitrogen fixation. African Journal of Biotechnology 11(8): 1886-1891.
  20. Simon, Z., Mtei, K., Gessesse, A., and Ndakidemi, P.A. (2014). Isolation and characterization of nitrogen fixing rhizobia from cultivated and uncultivated soils of Northern Tanzania. American Journal of Plant Sciences, 5(26):4050-4067
  21. Stajkoviæ, O., Deliæ, D., Jošiæ, D., Kuzmanoviæ, Ð., Rasuliæ, N., Kneževiæ-Vukèeviæ, J. (2011). Improvement of common bean growth by co-inoculation with Rhizobium and plant growth-promoting bacteria. Romanian Biotechnological Letters, 16(1): 5919-5926.
  22. Triadiati, Mubarik, N.R., and Ramasita, Y. (2013). Respon pertumbunan tanaman kedelai terhadap Bradyrhizobium japonicum toleran masam dan pemberian pupuk di tanah masam. J. Agron. Indonesia, 41(1): 24-31. 

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