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Research Article

volume 42 issue 1 (february 2019) : 32-38,   Doi: 10.18805/LR-3817

Molecular dissection of genetic diversity in pigeonpea  [Cajanus cajan (L) Millsp.] minicore collection

S
S. Muniswamy
R
R. Lokesha
R
Rachit Saxena
B
B. Fakrudin
K
Kishan Patel
Y
Yamanura
1Pulse Research Institute, Agricultural Research Station, Gulbarga-585 104, Karnataka, India.

  • Submitted19-12-2016|

  • Accepted12-01-2018|

  • First Online 16-07-2018|

  • doi 10.18805/LR-3817

Cite article:- Muniswamy S., Lokesha R., Saxena Rachit, Fakrudin B., Patel Kishan, Yamanura (2018). Molecular dissection of genetic diversity in pigeonpea [Cajanus cajan (L) Millsp.] minicore collection. Legume Research. 42(1): 32-38. doi: 10.18805/LR-3817.

ABSTRACT

The present investigation was carried out using 191genotypes as mini core collections of pigeonpea along with 5 check varieties to know the genetic diversity at molecular level. Significant variation was observed by the way of analysis of variance for nine characters viz., days to 50 % flowering, days to maturity, plant height, number of branches per plant, pod bearing length, number of pods per plant, number of seeds per pod, seed yield per plant and hundred seed weight. Molecular diversity using 18 polymorphic simple sequence repeat (SSR) markers divided genotypes into 15 clusters, of which ICP-11059 and AK-101 were solitary, indicating their distinctiveness among all genotypes. Similarly, BSMR-533, JKM-7, RVK-285, ICP-1126, ICP-348, ICP-6859 and ICP-7869 were found distinct among the genotypes. Geographical origin based diversity separated Indian and non Indian genotypes. The Un weighted Pair Group Method with Arithmetic mean (UPGMA) based dendrogram indicated distinctiveness of ICP-13633 and Bennur local, as they formed solitary cluster. The SSR marker CcM 602, as it could differentiate 4 genotypes at different base pair size can be used for identification and finger printing of genotypes.

REFERENCES

  1. Anderson, J.A., Churchill, G.A., Sutrique, J.E., Tanksley, S.D. and Sorrells, M.E., (1993), Optimizing parental selection for genetic linkage maps. Genome, 36: 181–186.
  2. Anonymous, 2014, Improved Agronomic Practices, UAS Dharwad and UAS Raichur., pp. 94-104
  3. Arunachalanm V.,(1981) Genetics distance in plant breeding, Indian J. Genet., 41:226-236
  4. Cuc, L.M., Mace, E,S., Crouch, J.H., Quang, V.D., Long, T.D., Varshney, R.K., (2008), Isolation and characterization of novel microsatellite markers and their application for diversity assessment in cultivated groundnut (Arachis hypogaea). BMC Plant Biology, 8: 55.
  5. Gupta, P. K. and Varshney, R. K., (2000), The development and use of microsatellite markers for genetic analysis and plant breeding with emphasis on bread wheat. Euphytica, 113: 163-185.
  6. http://www.faostat.fao.org. 
  7. Katiyar, P. K., Dua, R. P., Singh, I. P., Singh, B. B., Singh, F., (2004), Multivariate analysis for genetic diversity in early pigeonpea accessions. Legume Res., 27(3): 164-170.
  8. Metkar, A. P., Gawande, V. L. and Gahukar, S. J., (2010), Molecular characterization of CMS lines in pigeonpea (Cajanus cajan (L.) Millsp.). J. Food Legume, 23(3-4): 234-237.
  9. Neha, M. and Dinesh, Y., (2010), RAPD Analysis among pigeonpea [Cajanus Cajan (L).Millsp.] cultivar for their genetic diversity. J. Genet. Engineering Biotech., 1:1-9.
  10. Odeny, A., Jayashree, B., Ferguson, M., Hoisington, D., Crouch, J. and Gebhardt, C., (2007), Development, characterization and utilization of microsatellite markers in pigeonpea. Plant Breed., 126: 130–136.
  11. Panguluri, S. K., Janaiah, J., Govil, J. N., Kumar, P. A. and Sharma, P. C., (2006), AFLP fingerprinting in pigeonpea (Cajanus cajan (L.) Millsp.) and its wild relatives. Genet. Res. Crop. Evol., 53: 523- 531.
  12. Perrier, X., Flori, A. and Bonnot, F., (2003), Data Analysis Methods: Genetic diversity of cultivated tropical plants, Enfield Science Publishers, Montpellier, France. pp. 43-76.
  13. Reddy, L. J., Upadhyaya, H. D., Gowda, C. L. L. and Sube Singh, (2005), Development of core collection in Pigeonpea [Cajanus cajan (L.) Millsp.] using geographic and qualitative morphological descriptors. Genet. Res. Crop Evolut., 52: 1049–1056.
  14. Sarika, S. and Anand, R., (2013), Analysis of genetic diversity in pigeonpea (Cajanus cajan) by using PCR based molecular marker, Recent res. science Tech., 5(2): 20-23. 
  15. Saxena,. R. K., Sexana, K. B., Kumar, R. V., Hosington, D. A. and Varshney, R. K., (2010a), Simple sequence repeat-based diversity in elite pigeonpea genotypes for developing mapping populations to map resistance to Fusarium wilt and sterility mosaic disease, Plant Breed., 129: 135-141.
  16. Saxena, R. K, Saxena, K. and Varshney, R. K., (2010b), Application of SSR markers for molecular characterization of hybrid parents and purity assessment of ICPH 2438 hybrid of pigeonpea (Cajanus cajan (L.) Millsp.). Mol. Breed., 26: 371–380.
  17. Singh, A.K., Rai, V.P., Chand, R., Singh. R.P. and Singh, M.N., (2013), Genetic diversity studies and identification of SSR markers associated with Fusarium wilt (Fusarium udum) resistance in cultivated pigeonpea (Cajanus Cajan). J. Genet., 92(2): 273-279.
  18. Songok, S., Morag Ferguson, Anne, W. M. and Said Silim, (2010), Genetic diversity in pigeonpea [Cajanus cajan (L.) Millsp.]Landraces as revealed by simple sequence repeat markers. African J. Biotech., 9(22): 3231-3241.
  19. Songok, and Serah, C., (2013), Genetic diversity in pigeonpea (Cajanus cajan (L).Millsp.) landrace as revealed by simple sequence repeat markers, M.Sc. Thesis, Jomo Kenyatta Univ. Agric. Tech., Kenya, pp.126. 
  20. Upadhyaya, H. D., Reddy, K. N., Gowda, C. L. L. and Silim, S. N., (2007), Patterns of diversity in pigeonpea (Cajanus cajan (L.) MilMillsp.) germplasm collected from different elevations in Kenya. Genet. Res. Crop Evol., 54 (8): 1787-1795.
  21. Upadhyaya, H. D., Yadav, D., Dronavalli, N., Gowda, C. L. L. and Singh, S., (2010), Minicore germplasm collections for infusing genetic diversity in plant breeding programs. Elect. J. Plant Breed., 1(4): 1294-1309.
  22. Wasike, S., Okori, P. and Rubaihayo, P. R., (2005), Genetic variability and relatedness of the Asian and African pigeonpea as revealed by AFLP. African J. Biotech., 4: 1228-1233.
  23. Varshney, R. K., Graner, A., Sorrells, M. E., (2005), Genic microsatellite markers in plants: features and applications. Trends Biotechnol., 23: 48–55.
  24. Vos, P., Hoge R.S., Bleekers, R., Rejians, M., Van de Lee, M. T., Hornes, M., Frijters, A., et al. (1995), AFLP : A new technique for DNA fingerprinting. Nucleic Acids Res., 23: 4407-4414.
  25. Vetriventhan, M., Upadhyaya, H.D., Anandakumar, C.R., Senthilvel, S., Parzies, H.K., et al., (2012), Assessing genetic diversity, allelic richness and genetic relationship among races in ICRISAT foxtail millet core collection, Plant Genet. Res.: Characterization and Utilization, 10(3): 214-223.
  26. Yadav, K., Sanjay, K .Y., Anurag, Y., Pandey,V. P. and Dwivedi,U.N., (2012), Genetic diversity of pigeonpea (Cajanus cajan (L.) Millsp.) cultivars and its wild relatives using Randomly Amplified Polymorphic DNA (RAPD) Markers, Am. J. Plant Sci., 3: 322-330
  27. Yang, S.Y., Pang, W., Ash, G., Harper, J., Carling, J., Wenzl, P., Huttner, E., Zong, X.and Kilian, A., (2006), Low level of genetic diversity in cultivated pigeonpea compared to its wild relatives is revealed by diversity arrays technology. Theor. Appl. Genet., 113 : 585-595. 
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    Research Article

    volume 42 issue 1 (february 2019) : 32-38,   Doi: 10.18805/LR-3817

    Molecular dissection of genetic diversity in pigeonpea  [Cajanus cajan (L) Millsp.] minicore collection

    S
    S. Muniswamy
    R
    R. Lokesha
    R
    Rachit Saxena
    B
    B. Fakrudin
    K
    Kishan Patel
    Y
    Yamanura
    1Pulse Research Institute, Agricultural Research Station, Gulbarga-585 104, Karnataka, India.

    • Submitted19-12-2016|

    • Accepted12-01-2018|

    • First Online 16-07-2018|

    • doi 10.18805/LR-3817

    Cite article:- Muniswamy S., Lokesha R., Saxena Rachit, Fakrudin B., Patel Kishan, Yamanura (2018). Molecular dissection of genetic diversity in pigeonpea [Cajanus cajan (L) Millsp.] minicore collection. Legume Research. 42(1): 32-38. doi: 10.18805/LR-3817.

    ABSTRACT

    The present investigation was carried out using 191genotypes as mini core collections of pigeonpea along with 5 check varieties to know the genetic diversity at molecular level. Significant variation was observed by the way of analysis of variance for nine characters viz., days to 50 % flowering, days to maturity, plant height, number of branches per plant, pod bearing length, number of pods per plant, number of seeds per pod, seed yield per plant and hundred seed weight. Molecular diversity using 18 polymorphic simple sequence repeat (SSR) markers divided genotypes into 15 clusters, of which ICP-11059 and AK-101 were solitary, indicating their distinctiveness among all genotypes. Similarly, BSMR-533, JKM-7, RVK-285, ICP-1126, ICP-348, ICP-6859 and ICP-7869 were found distinct among the genotypes. Geographical origin based diversity separated Indian and non Indian genotypes. The Un weighted Pair Group Method with Arithmetic mean (UPGMA) based dendrogram indicated distinctiveness of ICP-13633 and Bennur local, as they formed solitary cluster. The SSR marker CcM 602, as it could differentiate 4 genotypes at different base pair size can be used for identification and finger printing of genotypes.

    REFERENCES

    1. Anderson, J.A., Churchill, G.A., Sutrique, J.E., Tanksley, S.D. and Sorrells, M.E., (1993), Optimizing parental selection for genetic linkage maps. Genome, 36: 181–186.
    2. Anonymous, 2014, Improved Agronomic Practices, UAS Dharwad and UAS Raichur., pp. 94-104
    3. Arunachalanm V.,(1981) Genetics distance in plant breeding, Indian J. Genet., 41:226-236
    4. Cuc, L.M., Mace, E,S., Crouch, J.H., Quang, V.D., Long, T.D., Varshney, R.K., (2008), Isolation and characterization of novel microsatellite markers and their application for diversity assessment in cultivated groundnut (Arachis hypogaea). BMC Plant Biology, 8: 55.
    5. Gupta, P. K. and Varshney, R. K., (2000), The development and use of microsatellite markers for genetic analysis and plant breeding with emphasis on bread wheat. Euphytica, 113: 163-185.
    6. http://www.faostat.fao.org. 
    7. Katiyar, P. K., Dua, R. P., Singh, I. P., Singh, B. B., Singh, F., (2004), Multivariate analysis for genetic diversity in early pigeonpea accessions. Legume Res., 27(3): 164-170.
    8. Metkar, A. P., Gawande, V. L. and Gahukar, S. J., (2010), Molecular characterization of CMS lines in pigeonpea (Cajanus cajan (L.) Millsp.). J. Food Legume, 23(3-4): 234-237.
    9. Neha, M. and Dinesh, Y., (2010), RAPD Analysis among pigeonpea [Cajanus Cajan (L).Millsp.] cultivar for their genetic diversity. J. Genet. Engineering Biotech., 1:1-9.
    10. Odeny, A., Jayashree, B., Ferguson, M., Hoisington, D., Crouch, J. and Gebhardt, C., (2007), Development, characterization and utilization of microsatellite markers in pigeonpea. Plant Breed., 126: 130–136.
    11. Panguluri, S. K., Janaiah, J., Govil, J. N., Kumar, P. A. and Sharma, P. C., (2006), AFLP fingerprinting in pigeonpea (Cajanus cajan (L.) Millsp.) and its wild relatives. Genet. Res. Crop. Evol., 53: 523- 531.
    12. Perrier, X., Flori, A. and Bonnot, F., (2003), Data Analysis Methods: Genetic diversity of cultivated tropical plants, Enfield Science Publishers, Montpellier, France. pp. 43-76.
    13. Reddy, L. J., Upadhyaya, H. D., Gowda, C. L. L. and Sube Singh, (2005), Development of core collection in Pigeonpea [Cajanus cajan (L.) Millsp.] using geographic and qualitative morphological descriptors. Genet. Res. Crop Evolut., 52: 1049–1056.
    14. Sarika, S. and Anand, R., (2013), Analysis of genetic diversity in pigeonpea (Cajanus cajan) by using PCR based molecular marker, Recent res. science Tech., 5(2): 20-23. 
    15. Saxena,. R. K., Sexana, K. B., Kumar, R. V., Hosington, D. A. and Varshney, R. K., (2010a), Simple sequence repeat-based diversity in elite pigeonpea genotypes for developing mapping populations to map resistance to Fusarium wilt and sterility mosaic disease, Plant Breed., 129: 135-141.
    16. Saxena, R. K, Saxena, K. and Varshney, R. K., (2010b), Application of SSR markers for molecular characterization of hybrid parents and purity assessment of ICPH 2438 hybrid of pigeonpea (Cajanus cajan (L.) Millsp.). Mol. Breed., 26: 371–380.
    17. Singh, A.K., Rai, V.P., Chand, R., Singh. R.P. and Singh, M.N., (2013), Genetic diversity studies and identification of SSR markers associated with Fusarium wilt (Fusarium udum) resistance in cultivated pigeonpea (Cajanus Cajan). J. Genet., 92(2): 273-279.
    18. Songok, S., Morag Ferguson, Anne, W. M. and Said Silim, (2010), Genetic diversity in pigeonpea [Cajanus cajan (L.) Millsp.]Landraces as revealed by simple sequence repeat markers. African J. Biotech., 9(22): 3231-3241.
    19. Songok, and Serah, C., (2013), Genetic diversity in pigeonpea (Cajanus cajan (L).Millsp.) landrace as revealed by simple sequence repeat markers, M.Sc. Thesis, Jomo Kenyatta Univ. Agric. Tech., Kenya, pp.126. 
    20. Upadhyaya, H. D., Reddy, K. N., Gowda, C. L. L. and Silim, S. N., (2007), Patterns of diversity in pigeonpea (Cajanus cajan (L.) MilMillsp.) germplasm collected from different elevations in Kenya. Genet. Res. Crop Evol., 54 (8): 1787-1795.
    21. Upadhyaya, H. D., Yadav, D., Dronavalli, N., Gowda, C. L. L. and Singh, S., (2010), Minicore germplasm collections for infusing genetic diversity in plant breeding programs. Elect. J. Plant Breed., 1(4): 1294-1309.
    22. Wasike, S., Okori, P. and Rubaihayo, P. R., (2005), Genetic variability and relatedness of the Asian and African pigeonpea as revealed by AFLP. African J. Biotech., 4: 1228-1233.
    23. Varshney, R. K., Graner, A., Sorrells, M. E., (2005), Genic microsatellite markers in plants: features and applications. Trends Biotechnol., 23: 48–55.
    24. Vos, P., Hoge R.S., Bleekers, R., Rejians, M., Van de Lee, M. T., Hornes, M., Frijters, A., et al. (1995), AFLP : A new technique for DNA fingerprinting. Nucleic Acids Res., 23: 4407-4414.
    25. Vetriventhan, M., Upadhyaya, H.D., Anandakumar, C.R., Senthilvel, S., Parzies, H.K., et al., (2012), Assessing genetic diversity, allelic richness and genetic relationship among races in ICRISAT foxtail millet core collection, Plant Genet. Res.: Characterization and Utilization, 10(3): 214-223.
    26. Yadav, K., Sanjay, K .Y., Anurag, Y., Pandey,V. P. and Dwivedi,U.N., (2012), Genetic diversity of pigeonpea (Cajanus cajan (L.) Millsp.) cultivars and its wild relatives using Randomly Amplified Polymorphic DNA (RAPD) Markers, Am. J. Plant Sci., 3: 322-330
    27. Yang, S.Y., Pang, W., Ash, G., Harper, J., Carling, J., Wenzl, P., Huttner, E., Zong, X.and Kilian, A., (2006), Low level of genetic diversity in cultivated pigeonpea compared to its wild relatives is revealed by diversity arrays technology. Theor. Appl. Genet., 113 : 585-595. 
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