Identification of an isoflavone glycoside hydrolyzing b-glucosidase in the seeds of the desert legume Prosopis cineraria

DOI: 10.18805/LR-3798    | Article Id: LR-3798 | Page : 837-841
Citation :- Identification of an isoflavone glycoside hydrolyzing b-glucosidase in the seeds of the desert legume Prosopis cineraria.Legume Research-An International Journal.2018.(41):837-841
V. Asati and P. K. Sharma pankajsharma@pilani.bits-pilani.ac.in
Address : Department of Biological Sciences, Birla Institute of Technology and Science (BITS), Pilani-333 031, Rajasthan, India
Submitted Date : 20-10-2016
Accepted Date : 28-06-2017

Abstract

Legumes, in general, are rich in glycosidic isoflavone conjugates, whereby specific b-glucosidases act to release the free aglycones that could serve in plant-microbe interactions and defense. Prosopis cineraria is an important legume tree, bearing medicinally useful and edible pods, generally growing in extreme arid/semi-arid zones of Rajasthan. However, specific phytochemical-cum-metabolic profiles are lacking for the same. Therefore, the present investigation undertook phytochemical and metabolic screening of the pods/seeds of P. cineraria for the presence of putative isoflavonoids viz.  genistein and daidzein, their glycosides and b-glucosidase(s) capable of catalyzing the glycoside hydrolysis.  Extraction and identification of  these  two  aglycone forms of  the above  isoflavonoids  were  performed  with  solvent  partition chromatography  and  Fluorescent/High  Performance Thin Layer Chromatography, respectively. Furthermore, optimization of the isoflavone conjugate-specific b-glucosidase activity with respect to pH optima, temperature and time were carried out. The partially purified enzyme showed a temperature optima of 50°C and pH optima of 4.5. The enzyme also demonstrated activity towards natural substrates daidzin and genistin which are glycosides of isoflavonoids daidzein and genistein respectively. The methanolic extracts of the seeds of P. cineraria indicated the presence of related isoflavonoids which needs to be further validated.

Keywords

Glycoside b-Glucosidase Isoflavonoid Prosopis cineraria

References

  1. Beniwal, P. and Jood, S. (2014) Total phenolic content and antioxidant activity of byproducts from cereal and legume milling industries. Asian Journal of Dairy and Food Research, 33: 307-310.
  2. Chuankhayan, P., Rimlumduan, T., Svasti, J. and Cairns, J.R.K. (2007) Hydrolysis of soybean isoflavonoid glycosides by Dalbergia â-    glucosidases. Journal of Agricultural and Food Chemistry, 55: 2407-2412.
  3. Dewick, P.M. (1994) Isoflavones. In:The Flavonoids: Advances in research since 1986, 1st ed.; Harborne JB, ed; Chapman and Hall: London, UK, pp 117-238.
  4. Hosel, W. and Barz, W. (1975) b-Glucosidases from Cicer arietinum L.: purification and properties of isoflavone-7-O-glucoside-    specific b-glucosidases. European Journal of Biochemistry, 57: 607-616.
  5. Hsieh, M.C. and Graham, T.L. (2001) Partial purification and characterization of a soybean b-glucosidase with high specific activity towards isoflavone conjugates. Phytochemistry, 58: 995-1005.
  6. Jain, P. (2011) “The Bishnoi Community”, In: Dharma and Ecology of Hindu Communities, Ashgate Publishing Ltd., England, pp. 51.
  7. Lepiniec, L., Debeaujon, I., Routaboul, J-M., Baudry, A., Pourcel, L., Nesi, N. and Caboche, M. (2006) Genetics and biochemistry of Seed Flavonoids. Annual Review of Plant Biology, 57: 405-430.
  8. Li, X. H., Zhang, J. C., Sui, S. F., and Yang, M. S. (2005). Effect of daidzin, genistin, and glycitin on osteogenic and adipogenic differentiation of bone marrow stromal cells and adipocytic transdifferentiation of osteoblasts. Acta Pharmacologica Sinica, 26: 1081–1086. 
  9. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951) Protein measurement with the Folin-phenol reagent. Journal of Biological Chemistry, 193: 265-275.
  10. Messina, M. and Barnes, S. (1991) The role of soy products in reducing risk of cancer. Journal of the National Cancer Institute, 83: 541-546.
  11. Narayan,P. and Kumar, S. (2015) Constraints of growth in area production and productivity of pulses in India: An analytical approach to major pulses. Indian Journal of Agricultural Research, 49: 114-124.
  12. Purohit, S.D., Ramawat, K.G. and Arya, H.C. (1979) Phenolics, peroxidase and phenolase as related to gall formation in some arid zone plants. Current Science, 48: 714-16.
  13. Rathore, M. (2009) Nutrient content of important fruit trees from arid zone of Rajasthan. Journal of Horticulture and Forestry, 1: 103-108.
  14. Setchell, K.D., Brown, N.M., Zimmer-Nechemias, L., Brashear, W.T., Wolfe, B.E., Kirschner, A.S. and Heubi, J.E. (2002) Evidence for lack of absorption of soy isoflavone glycosides in humans, supporting the crucial role of intestinal metabolism for bioavailability. American Journal of Clinical Nutrition, 76: 447-453.
  15. Sharma, N., Garg, V. and Paul, A. (2010) Antihyperglycemic, antihyperlipidemic and antioxidative potential of Prosopis cineraria bark. Indian Journal of Clinical Biochemistry, 25: 193-200.
  16. Singh, G. (2009) Comparative productivity of Prosopis cineraria and Tecomella undulata based agroforestry system in degraded lands of Indian Desert. Journal of Forestry Research, 20: 144-150.
  17. Sinha, S. K., Kumar, M., Kumar, A., Bharti, S. and Shahi, V. K. (2013) Antioxidant activities of different tissue extract of Faba bean (Vicia faba L .) containing phenolic compounds. Legume Research-An International Journal, 36: 496–504.    Srisomsap, C., Svasti, J., Surarit, R., Champattanachai, V., Sawangareetrakul, P., Boonpuan, K., Subhasitanont, P. and Chokchaichamnankit, D. (1996) Isolation and characterization of an enzyme with â-glucosidase and â-fucosidase activities from Dalbergia cochinchinensis Pierre. Journal of Biochemistry, 119: 585-590.
  18. Wang, Q., Ge, X., Tian, X., Zhang, Y., Zhang, J. and Zhang, P. (2013) Soy isoflavone: the multipurpose phytochemical. Biomedical Reports, 1: 697-701.
  19. Zhu, D., Hettiarachchy, N.S., Horax, R.and Chen, P. (2005) Isoflavone contents in germinated soybean seeds. Plant Foods for Human Nutrition, 60: 147-151.

Global Footprints