Legume Research

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Legume Research, volume 40 issue 4 (august 2017) : 601-608

Role of Genomic tools for Mungbean [Vigna radiata (L.) Wilczek] improvement

Vijayata Singh, N. R. Yadav, Jogendra Singh
1<p>ICAR-Central Soil Salinity Research Institute,&nbsp;Karnal-132001, Haryana, India</p>
Cite article:- Singh Vijayata, Yadav R. N., Singh Jogendra (2017). Role of Genomic tools for Mungbean [Vigna radiata (L.) Wilczek] improvement . Legume Research. 40(4): 601-608. doi: 10.18805/lr.v0i0.8406.

Molecular markers are routinely utilized worldwide in all major crops as a component of breeding. The pace of development of molecular markers, establishment of marker–trait associations for important agronomic traits and other genomic sources has been accelerated in other pulses than the mungbean. The efforts are underway to use high-throughput genotyping platforms besides developing more genomic resources. So far, progress in the use of marker-assisted selection as a part of mungbean breeding programmes has been very limited. In this article, we have reviewed the progress made, limitations encountered and future possibilities for the application of marker-assisted selection in the genetic improvement of mungbean crops.


  1. Blair, M.W., Pedraza, F., Buendia, H.F., Gaitán-Solís, E., Beebe, S.E., Gepts, P. and Tohme, J. (2003). Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.). Theor. Appl. Genet. 107: 81362-74.

  2. Bordat, A., Savois, V., Nicolas, M., Salse, J., Chauveau, A. and Bourgeois, M. (2011). Translational genomics in legumes allowed placing insilico 5460 unigenes on the pea functional map and identified candidate genes in Pisum sativum L. G3 1: 93–103.

  3. Boutin, S.R., Young, N.D., Olson, T.C., Yu, Z.H., Shoemaker, R.C. and Vallejos, C.E. (1995). Genome conservation among three legume genera detected with DNA markers. Genome 38: 928–37.

  4. Brumfield, R.T., Beerli, P., Nickerson, D.A., and Edwards, S.V. (2003). The utility of single nucleotide polymorphisms in inferences of population history. Trends Ecol. Evol. 18: 249–256.

  5. Cannon, S. B., Sterck, L., Rombauts, S., Sato, S., Cheung, F. and Gouzy, J. (2006). Legume genome evolution viewed through the Medicago truncatula and Lotus japonicus genomes. Proc. Natl. Acad. Sci. U.S.A. 103:14959–14964. 

  6. Chaitieng, B., Kaga, A., Tomooka, N., Isemura, T., Kuroda, Y. and Vaughan, D.A. (2006). Development of a black gram [Vigna mungo (L.) Hepper] linkage map and its comparison with an azuki bean [Vigna angularis (Willd.) Ohwi and Ohashi] linkage map. Theor. Appl. Genet. 113:1261-1269.

  7. Chen, X., Laudeman, T., Rushton, P., Spraggins, T. and Timko, M. (2007). CGKB: an annotation knowledge base for cowpea (Vigna unguiculata L.) methylation filtered genomic gene space sequences. BMC Bioinformatics 8:129.

  8. Choi, H.K., Mun, J.H., Kim, D.J., Zhu, H., Baek, J.M., Mudge, J., Roe, B., Ellis, N., Doyle, J., Kiss, G.B., Young, N.D. and Cook, D.R. (2004). Estimating genome conservation between crop and model legume species. Proc. Nat. Acad. Sci. USA 101: 15289–94.

  9. Fatokun, C.A., Danesh, D., Young, N.D. (1993). Molecular taxonomic relationships in the genus Vigna based on RFLP analysis. Theor. Appl. Genet. 86 (1):97-104

  10. Fernandez, G.C.J. and Shanmusgasundaram, S. (1988). The AVRDC mungbean improvement program: The past present and Future. In: Proceedings of the second international symposium. Bangkok, Thailand. Pp 58-70.

  11. Gepts, P., Beavis, W.D., Brummer, E.C., Shoemaker, R.C., Stalker, H.T. and Weeden, N.F. (2005). Legumes as a model plant family. Genomics for food and feed report of the cross-legume advances through genomics conference. Plant Physiol. 137: 1228–1235.

  12. Gwag, J.G., Chung, W.K., Chung, H. K., Lee, J.H., Ma, K.H., Dixit, A., Park, Y.J., Cho, E.G., Kim, T.S. and Lee, S.H. (2006). Characterization of new microsatellite markers in mungbean. Mol. Ecol. Notes 6: 1132-1134.

  13. Han, O.K., Kaga, A., Isemura, I., Wang, X.W., Tomooka, N. and Vaughan, D.A. (2005). A genetic linkage map for azuki bean [Vigna angularis (Willd) Ohwi & Ohashi]. Theor. Appl. Genet. 111: 1278-1287.

  14. Hong, E.H., Lee, Y.H., Kim, S.D., Hwang, Y.H., Moon, Y.H., and Ham, Y.S.(1983). New disease resistant and high yielding Mungbean variety “Seonhwanogdu.” Res. Rept. 25: 178–180.

  15. Humphry, M.E., Konduri, V., Lambrides, C.J., Magner, T., McIntyre, C.L., Aitken, E.A.B. and Liu, C.J. (2002). Development of a Mungbean [Vigna radiata L. Wilczek] RFLP linkage map and its comparison with lablab, Lablab purpureus reveal a high level of colinearity between the two genomes. Theor. Appl. Genet. 105: 160-166.

  16. Isemura, T., Kaga, A., Konishi, S., Ando, T., Tomooka, N., Han, O.K. and Vaughan, D.A. (2007). Genome dissection of traits related to domestication in azukibean, Vigna angularis and comparison with other warm- season legumes. Ann. of Bot. 100: 1053-1071.

  17. Isemura, T., Kaga, A., Tabata, S., Somta, P. and Srinives, P. (2012). Construction of a genetic linkage map and genetic analysis of domestication related traits in Mungbean (Vigna radiata). PLoS ONE 7: e41304.

  18. Jung, C.H., Wong, C.E., Singh, M.B. and Bhalla, P.L. (2012). Comparative genomic analysis of soybean flowering genes. PLoS ONE 7: e38250.

  19. Kaga, A., Ishii, T., Tsukimoto, K., Tokoro, E. and Kamijima, O. (2000). Comparative molecular mapping in Ceratotropis species using an interspecific cross between azuki bean (Vigna angularis) and rice bean (V. umbellata). Theor. Appl. Genet. 100:207-213.

  20. Kang, Y.J., Kim, S.K., Kim, M.Y., Lestari, P., Kim, K.H. and Ha, B.K. (2014). Genome sequence of Mungbean and insights into evolution within Vigna species. Nat. Commun. 5: 5443. 

  21. Kang, Y.J., Satyawan, D., Shim, S., Lee, T., Lee, J. and Hwang, W.J. (2015). Draft genome sequence of adzukibean, Vigna angularis. Sci. Rep. 5: 8069.

  22. Karuppanapandian, T., Karuppudurai, T., Sinha, T.P. M., HamarulHaniya, A. and Manoharan, K. (2006). Genetic diversity in green gram [Vigna radiata (L).] landraces analyzed by using random amplified polymorphic DNA, RAPD. Afr. J. Biotechnol. 5: 1214 -1219.

  23. Khattak, A. B, Bibi, N. and Aurangzeb. (2007). Quality assessment and consumers acceptability studies of newly evolved Mungbean [Vigna radiata (L.)] genotypes. Am. J. of Food Tech. 2(6): 536-542.

  24. Kim, S.K., Lee, T., Kang, Y.J., Hwang, W.J., Kim, K.H. and Moon, J.K. (2014). Genome-wide comparative analysis of flowering genes between Arabidopsis and Mungbean. Genes Genomics 36: 799–808.

  25. Kwak, M., Velasco, D. and Gepts, P. (2008). Mapping homologous sequences for determinacy and photoperiod sensitivity in commonbean (Phaseolus vulgaris). J. Hered. 99: 283–291.

  26. Lambrides, C. J., Lawn, R. J., Godwin, I. D., Manners, J. and Imrie, B. C. (2000). Two genetic linkage maps of Mungbean using RFLP and RAPD markers. Aust. J. Agri. Res. 51: 415–425. 

  27. Lee, J.M., Grant, D., Vallejos, C.E. and Shoemaker, R.C. (2001).Genome organization in dicots. II. Arabidopsis as a bridging species to resolve genome duplication events among legumes. Theor. Appl. Genet. 103: 765–73.

  28. Li, C.D., Fatokun, C.A., Ubi, B., Singh, B.B. and Scoles, G.J. (2001). Determining genetic similarities and relationships among cowpea breeding lines and cultivars by microsatellite markers. Crop Sci. 41: 189–197.

  29. Liu, L., Li, Y., Li, S., Hu, N., He, Y. and Pong, R. (2012).Comparison of next-generation sequencing systems. J. Biomed. Biotechnol. 2012: 251-364. 

  30. Luo, C., Tsementzi, D., Kyrpides, N., Read, T. and Konstantinidis, K. T. (2012). Direct comparisons of Illumina vs. Roche 454 sequencing technologies on the same microbial community DNA sample. PLoS ONE 7: e30087. 

  31. Menancio-Hautea, D., Fatokun, C. A., Kumar, L., Danesh, D. and Young, N. D. (1993). Comparative genome analysis of Mungbean (Vigna radiata L. Wilczek) and cowpea (Vigna unguiculata L. Walpers) using RFLP mapping data.Theor. Appl. Genet. 86 :797–810. 

  32. Miyagi, M., Humphry, M., Ma, Z.Y., Lambrides, C.J., Bateson, M. and Liu, C.J. (2004).Construction of bacterial artificial chromosome libraries and their application in developing PCR-based markers closely linked to a major locus conditioning bruchid resistance in Mungbean [Vigna radiata (L.) Wilczek]. Theor. Appl. Genet. 110: 151–156.

  33. Moe, K.T., Chung, J.W., Cho, Y.I., Moon, J.K., Ku, J.H. and Jung, J.K. (2011). Sequence information on simple sequence repeats and single nucleotide polymorphisms through transcriptome analysis of Mungbean. J. Integr. Plant Biol. 53: 63–73.

  34. Mudge, J., Cannon, S.B., Kalo, P., Oldroyd, G.E., Roe, B.A. and Town, C.D. (2005). Highly syntenic regions in the genomes of soybean Medicago truncatula and Arabidopsis thaliana. BMC Plant Biol. 5: 15. 

  35. Ramiirez, M., Graham, M.A., Blanco-Loìpez, L., Silvente, S., Medrano-Soto, A., Blair, M.W., Hernaìndez, G., Vance, C.P. and Lara, M. (2005). Sequencing and analysis of common bean ESTs to build a foundation for functional genomics. Pl. Physiol. 137: 1211–1227.

  36. Rishi, N. (2009). Significant plant virus diseases in India and a glimpse of modern disease management technology. J. Gen. Plant Pathol. 75: 1–18.

  37. Sato, S., Nakamura, Y., Kaneko, T., Asamizu, E., Kato, T. and Nakao, M. (2008). Genome structure of the legume, Lotus japonicus. DNA Res. 15: 227–239. 

  38. Schmutz, J., Cannon, S.B., Schlueter, J., Ma, J., Mitros, T.,Nelson, W. (2010). Genome sequence of the palae-opolyploid soybean. Nature 463: 178–183.

  39. Schmutz, J., McClean, P.E., Mamidi, S., Wu, G.A., Cannon, S.B. and Grimwood, J. (2014).A reference genome for common bean and genome-wide analysis of dual domestications. Nat. Genet. 46: 707–713.

  40. Singh, D.P. and Ahlawat, I.P.S. (2005). Greengram (Vigna radiate) and blackgram (V. mungo) improvement in India past, present and future prospects. Indian J. of Agri. Sci. 75: 243-50.

  41. Somta, P. and Srinives. P. (2007). Genome research in Mungbean (Vigna radiata (L.) Wilczek) and blackgram (V. mungo (L.) Hepper). Science Asia. 33 (1): 69–74.

  42. Somta, P., Ammaranan, C., Peter, A. C. O. and Srinives, P. (2007). Inheritance of seed resistance to bruchids in cultivated Mungbean (Vigna radiata L. Wilczek). Euphytica 155: 47–55. 

  43. Somta, P., Kaga, A.., Tomooka, N., Kashiwaba, K., Isemura, T., Chaitieng, B., Srinives, P. and Vaughan, D.A. (2006). Development of an interspecific Vigna linkage map between Vigna umbellate [(Thunb.) Ohwi & Ohashi] and V. nakashimae [(Ohwi & Ohashi)] and its use in analysis of bruchid resistance and comparative genomics.Pl. Breed. 125: 77–84.

  44. Stacey, G. and VandenBosch, K. (2005). ‘Translational’ legume biology. Models to crops. Plant Physiol. 137: 1173.

  45. Tomooka, N., Kaga, A. and Vaughan, D.A. (2006a). The Asian Vigna (Vigna subgenus Ceratotropis) biodiversity and evolution. In: Sharma, A. K. and Sharma, A. (eds) Plant genome: biodiversity and evolution. Part C: phanerogams (angiosperms– dicotyledons), vol 1. Science, Enfield, Pp. 87–126.

  46. Tomooka, N., Vaughan, D.A. and Kaga, A. (2005).Mungbean [Vigna radiata (L.)Wilczek]. In: Singh R.J., Jauhar, P.P. (eds.). Genetic resources, chromosome engineering and crop improvement. II. Grain legumes. CRC, Boca Raton. Pp 319–339

  47. Van, K., Kang, Y.J., Han, K. S., Lee, Y.H., Gwang, J.G. and Moon, J.K. (2013). Genome-wide SNP discovery in Mungbean by Illumina HiSeq. Theor. Appl. Genet. 126: 2017–2027.

  48. Varshney, R.K., Chen, W., Li, Y., Bharti, A.K., Saxena, R.K. and Schlueter, J.A. (2012). Draft genome sequence of pigeonpea (Cajanus cajan), an orphan legume crop of resource-poor farmers. Nat. Biotechnol. 30: 83–89. 

  49. Varshney, R.K., Graner, A. and Sorrells, M.E. (2005). Genomics-assisted breeding for crop improvement. Trends Plant Sci. 10: 621–630.

  50. Varshney, R.K., Kudapa, H., Pazhamala, L., Chitikineni, A., Thudi, M. and Bohra, A. (2015). Translational genomics in agriculture : some examples in grain legumes. CRC Crit. Rev. Plant Sci. 34: 169–194. 

  51. Varshney, R.K., Song, C., Saxena, R.K., Azam, S., Yu, S. and Sharpe, A.G. (2013). Draft genome sequence of chickpea (Cicer arietinum) provides are source for trait improvement. Nat. Biotechnol. 31: 240–246. 

  52. Yan, H.H., Mudge, J., Kim, D.J., Shoemaker, R.C., Cook, D.R. and Young N.D. (2004). Comparative physical mapping reveals features of microsynteny between Glycine max, Medicago truncatula, and Arabidopsis thaliana. Genome 47: 141–155.

  53. Yang, S., Gao, M.., Xu, C., Gao, J., Deshpande, S. and Lin, S. (2008). Alfalfa benefits from Medicago truncatula: the RCT1 gene from M. truncatula confers broad-spectrum resistance to anthracnose in alfalfa. Proc. Natl. Acad. Sci. U.S.A. 105 12164–12169.

  54. Young, N. D., Kumar, L., Menancio-Hautea, D., Danesh, D., Talekar, N. S. and Shanmugasundaram, S. (1992). RFLP mapping of major bruchid resistance gene in Mungbean (Vigna radiata). Theor. Appl. Genet. 84:839–844. 

  55. Young, N.D., Debellé, F., Oldroyd, G.E.D., Geurts, R., Cannon, S.B. and Udvardi, M.K. (2011).The Medicago genome provides insight into the evolution of rhizobial symbioses. Nature 480: 520–524.

  56. Zhao, D., Xu-Zhen, C., Li-Xia, W., Su-Hua, W. and Yan-Ling, M. (2010). Construction of Mungbean Genetic Linkage Map. Acta Agro. Sinica. 36 (6): 932–939.

     

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