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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 41 issue 3 (june 2018) : 399-404,   Doi: 10.18805/LR-377

Interaction effects of fructan and Salicylic acid on chickpea in both biochemical and traditional agronomic indicators
 

N
N. Candan Yücel
1Department of Chemistry, Dokuz Eylül University, Faculty of Science, Buca, 35390, Izmir, Turkey.

  • Submitted04-07-2017|

  • Accepted18-11-2017|

  • First Online 23-01-2018|

  • doi 10.18805/LR-377

Cite article:- Yücel Candan N. (2018). Interaction effects of fructan and Salicylic acid on chickpea in both biochemical and traditional agronomic indicators. Legume Research. 41(3): 399-404. doi: 10.18805/LR-377.

ABSTRACT

Starch and fructans are accumulated for carbohydrate storage in legumes, while fructans accumulated large amounts than starch. The uses of this natural and biodegradable material counteract stress as cheaper and safer alternatives. Therefore, fructan (F, 0.5 %) and salicylic acid (SA, 0.5 mM) priming were used as exogenous growth enhancers to stimulate chickpea (Cicer arietinum L.) seed vigor against salt stress. The main aim of this study was to address whether priming chickpea with F, SA and F+SA could bring about supplementary benefits particularly against salt stress. Exogenous application of F- or SA-alone improved chickpea development in the presence of salt stress. Nevertheless, the best results in terms of growth, seed vigor and total phenolic – flavonoids, chlorophyll – carotenoids  contents, phenylalanine ammonia-lyase (PAL), ascorbic acid oxidase (AAO) activities and lipid peroxidation level (LPO) were determined in the combined F+SA treatment against salt stress. 

REFERENCES

  1. Arun, M.N., Bhanuprakash, K., Hebbar, S.S. and Senthivel, T. (2017). Effect of seed priming on biochemical parameters and seed germination in cowpea (Vigna unguiculata L.) walp. Legume Research 40:562-570.
  2. Bradford, M.M. (1976). A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254.
  3. Buege, J. A. and Aust, S. D. (1978). Microsomal lipid peroxidation. Methods in Enzymology 52: 302–310.
  4. Caputo, E., Ceglie, V., Lippolis, M., Rocca, N. and De Tullio, M. C. (2010). Identification of a NaCl-induced ascorbate oxidase activity from Chaetamorpha linum suggests a novel mechanism of adaptation to increased salinity. Environmental and Experimental Botany 69:63-67.
  5. Conrath, U. (2011). Moleular aspects of defence priming. Trends in Plant Science 16:524-531.
  6. Du, L., Ali, G.S., Simons, K.A., Hou, J., Yang, T., Reddy, A.S. and Poovanah, B. (2009). Ca2+/calmodulin regulates salicylic-acid-    mediated plant immunity. Nature 457:1154-1158. 
  7. Ende, W. (2013). Multifunctional fructans and raffinose family oligosaccharides. Plant Science 247: 1-10.
  8. Fotopoulos, V., De Tullio, M.C., Barnes, J. and Kanellis, A. K. (2008). Altered stomatal dynamics in ascorbate oxidase over-expressing tobacco plants suggest a role for dehydroascorbate signalling. Journal of Experimental Botany 59:729-737. 
  9. Gomez-Ariza, J., Campo, S., Rufat, M., Estopa, M., Messeguer, J., San Segundoa, B. and Coca, M. (2007). Sucrose-mediated priming of plant defense responses and broad-spectrum disease resistance by overexpression of the maize pathogenesis-related PRms protein in rice plants. Molecular Plant Microbial Interaction 20:832–842.
  10. Hendry, G.A.F. (1993). Evolutionary origins and functions of fructan—a climatologically. New Phytology 123:3–14.
  11. Hodgins, D.S. (1971). Yeast phenylalanine ammonia-lyase. Purification, properties, and the identification of catalytically essential dehydroalanine. Journal of Biology and Chemistry 246:2977–2985.
  12. Oberbacher, M.F. and Vines, H.M. (1963). Spectrophotometric assay of ascorbic acid oxidase. Nature 197:1203-1204.
  13. Pontis, H.G. (1989). Fructans and cold stress. Journal of Plant Physiology 134:148–150.
  14. Kerepesi, I., Galiba, G. and Banyai, E. (1998). Osmotic and salt stresses induced differential alteration in water-soluble carbohydrate content in wheat seedlings. Journal of Agricultural Food Chemistry 46:5347-5354.
  15. Kumar, M.S., Devi, R.S.J., Reddy, B. and Prasanthi, L. (2017). Morphological and cultural characterization of colletotrichum capsici, incitant of blight of chickpea in Andhra Pradesh, India. Legume Research 40:592-596.
  16. McCue, P., Zheng, Z., Pinkham, J. L. and Shetty, K. (2000). A model for enhanced pea seedling vigour following low pH and salicylic acid treatments. Process Biochemistry 35: 60-613.
  17. Nakano, Y. and Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiology 22:867–880.
  18. Pourcel, L., Irami, N.G., Koo, A.K., Bohorquez-Restrepo, A., Howe, G.A. and Grotewold, E. (2013). A chemical complementation approach reveals genes and interactions of flavonoids with other pathways. Plant Journal 74:383-397.
  19. Shalitin, A. and Wolf, C. (2000). Cucumber mosaic virus infection affects sugar transport in melon plants. Plant Physiology 123:597-604.
  20. Salerno, G.L., Porchia, A.C., Vargas, W. and Abdian, P.L. (2004). Fructose-containing oligosaccharides: Novel compatible solutes in Anabaena cells exposed to salt stress. Plant Science 167:1003–1008.
  21. Song, J.Y. and Roe, J.H. (2008). The role and regulation of Trxl, a cytosolic thioredoxin in Schizosacchoromyces pombe. Journal of Microbiology 46:408–414.
  22. Zhang, U. (2011). Moleular aspects of defence priming. Trends in Plant Science 16:524-531.
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    Research Article

    volume 41 issue 3 (june 2018) : 399-404,   Doi: 10.18805/LR-377

    Interaction effects of fructan and Salicylic acid on chickpea in both biochemical and traditional agronomic indicators
     

    N
    N. Candan Yücel
    1Department of Chemistry, Dokuz Eylül University, Faculty of Science, Buca, 35390, Izmir, Turkey.

    • Submitted04-07-2017|

    • Accepted18-11-2017|

    • First Online 23-01-2018|

    • doi 10.18805/LR-377

    Cite article:- Yücel Candan N. (2018). Interaction effects of fructan and Salicylic acid on chickpea in both biochemical and traditional agronomic indicators. Legume Research. 41(3): 399-404. doi: 10.18805/LR-377.

    ABSTRACT

    Starch and fructans are accumulated for carbohydrate storage in legumes, while fructans accumulated large amounts than starch. The uses of this natural and biodegradable material counteract stress as cheaper and safer alternatives. Therefore, fructan (F, 0.5 %) and salicylic acid (SA, 0.5 mM) priming were used as exogenous growth enhancers to stimulate chickpea (Cicer arietinum L.) seed vigor against salt stress. The main aim of this study was to address whether priming chickpea with F, SA and F+SA could bring about supplementary benefits particularly against salt stress. Exogenous application of F- or SA-alone improved chickpea development in the presence of salt stress. Nevertheless, the best results in terms of growth, seed vigor and total phenolic – flavonoids, chlorophyll – carotenoids  contents, phenylalanine ammonia-lyase (PAL), ascorbic acid oxidase (AAO) activities and lipid peroxidation level (LPO) were determined in the combined F+SA treatment against salt stress. 

    REFERENCES

    1. Arun, M.N., Bhanuprakash, K., Hebbar, S.S. and Senthivel, T. (2017). Effect of seed priming on biochemical parameters and seed germination in cowpea (Vigna unguiculata L.) walp. Legume Research 40:562-570.
    2. Bradford, M.M. (1976). A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254.
    3. Buege, J. A. and Aust, S. D. (1978). Microsomal lipid peroxidation. Methods in Enzymology 52: 302–310.
    4. Caputo, E., Ceglie, V., Lippolis, M., Rocca, N. and De Tullio, M. C. (2010). Identification of a NaCl-induced ascorbate oxidase activity from Chaetamorpha linum suggests a novel mechanism of adaptation to increased salinity. Environmental and Experimental Botany 69:63-67.
    5. Conrath, U. (2011). Moleular aspects of defence priming. Trends in Plant Science 16:524-531.
    6. Du, L., Ali, G.S., Simons, K.A., Hou, J., Yang, T., Reddy, A.S. and Poovanah, B. (2009). Ca2+/calmodulin regulates salicylic-acid-    mediated plant immunity. Nature 457:1154-1158. 
    7. Ende, W. (2013). Multifunctional fructans and raffinose family oligosaccharides. Plant Science 247: 1-10.
    8. Fotopoulos, V., De Tullio, M.C., Barnes, J. and Kanellis, A. K. (2008). Altered stomatal dynamics in ascorbate oxidase over-expressing tobacco plants suggest a role for dehydroascorbate signalling. Journal of Experimental Botany 59:729-737. 
    9. Gomez-Ariza, J., Campo, S., Rufat, M., Estopa, M., Messeguer, J., San Segundoa, B. and Coca, M. (2007). Sucrose-mediated priming of plant defense responses and broad-spectrum disease resistance by overexpression of the maize pathogenesis-related PRms protein in rice plants. Molecular Plant Microbial Interaction 20:832–842.
    10. Hendry, G.A.F. (1993). Evolutionary origins and functions of fructan—a climatologically. New Phytology 123:3–14.
    11. Hodgins, D.S. (1971). Yeast phenylalanine ammonia-lyase. Purification, properties, and the identification of catalytically essential dehydroalanine. Journal of Biology and Chemistry 246:2977–2985.
    12. Oberbacher, M.F. and Vines, H.M. (1963). Spectrophotometric assay of ascorbic acid oxidase. Nature 197:1203-1204.
    13. Pontis, H.G. (1989). Fructans and cold stress. Journal of Plant Physiology 134:148–150.
    14. Kerepesi, I., Galiba, G. and Banyai, E. (1998). Osmotic and salt stresses induced differential alteration in water-soluble carbohydrate content in wheat seedlings. Journal of Agricultural Food Chemistry 46:5347-5354.
    15. Kumar, M.S., Devi, R.S.J., Reddy, B. and Prasanthi, L. (2017). Morphological and cultural characterization of colletotrichum capsici, incitant of blight of chickpea in Andhra Pradesh, India. Legume Research 40:592-596.
    16. McCue, P., Zheng, Z., Pinkham, J. L. and Shetty, K. (2000). A model for enhanced pea seedling vigour following low pH and salicylic acid treatments. Process Biochemistry 35: 60-613.
    17. Nakano, Y. and Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiology 22:867–880.
    18. Pourcel, L., Irami, N.G., Koo, A.K., Bohorquez-Restrepo, A., Howe, G.A. and Grotewold, E. (2013). A chemical complementation approach reveals genes and interactions of flavonoids with other pathways. Plant Journal 74:383-397.
    19. Shalitin, A. and Wolf, C. (2000). Cucumber mosaic virus infection affects sugar transport in melon plants. Plant Physiology 123:597-604.
    20. Salerno, G.L., Porchia, A.C., Vargas, W. and Abdian, P.L. (2004). Fructose-containing oligosaccharides: Novel compatible solutes in Anabaena cells exposed to salt stress. Plant Science 167:1003–1008.
    21. Song, J.Y. and Roe, J.H. (2008). The role and regulation of Trxl, a cytosolic thioredoxin in Schizosacchoromyces pombe. Journal of Microbiology 46:408–414.
    22. Zhang, U. (2011). Moleular aspects of defence priming. Trends in Plant Science 16:524-531.
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