Legume Research-An International Journal
Publish
your articles with us

Quick Facts



Payment Options

payment portals

Click here to pay directly

Effects of seed priming on biochemical parameters and seed germination in cowpea [vigna unguiculata (L.) Walp]
 

M.N. Arun*, K. Bhanuprakash, S. Shankar Hebbar and T. Senthivel

ICAR – Indian Institute of Horticulture Research, Hesaraghatta, Bengaluru- 560 089, India

arun_tulasi2011@yahoo.in

Page Range:
562-570
Article ID:
LR-3724
Online Published:
16-05-2017
Abstract

Cowpea seeds lose their vigour during storage at high temperature and high relative humidity. Present study was conducted to invigorate the performance of high and low vigour seeds (exposed to 45 ± 2o C and 100% RH) by seed priming with different chemicals and concentrations (GA3 (100ppm); CaCl2 (10-3M); Ammonium Molybdate (10-3M); KBr (10-3M); Mg (NO3)2 (10-3M); ZnSO4 (10-3M); hydro primed and dry seed (control) at 15o C for 24 hours. Priming was effective in reducing the time for 50 % germination and mean germination time and increased the germination percentage in low vigour seeds, while energy of germination and final germination percentage in normal seeds displayed a minor increase. Priming of normal / low – vigour seeds improved the vigour of seedling in terms of seedling length and their dry weight and seedling vigour index. Seed priming of fresh and accelerated cowpea seeds increased the electrical conductivity of seed leachate, cell membrane stability, total protein content, a – amylase activity, peroxidase activity and dehydrogenase activity over control dry seeds. Esterase and peroxidase enzymes were completely lost as a result of accelerated ageing, which showed reappearance after priming. Priming showed little improvement in the banding pattern and intensity of enzyme in normal seeds. 
 

Keywords
Accelerated aging, Seed priming , Vigna Unguiculata (L.), Vigour, Walp.
References
  1. Abdul - baki, A. A. and Anderson, J. D. (197)3. Vigour determination in soybean by multiple criteria. Crop Science, 13: 630-633.
  2. Anuradha, V., Alice, K.V. and Malavika, D. (2010). The sub - cellular basis of seed priming. Current Science, 99: 450 “ 456.
  3. Bartlett, M.S., (1973). Some examples of Statistical Methods of Research in Applied Biology. Journal of Research and Statistical Society 4: 137-183.
  4. Basra, S. M., Farooq, M., Tabbassum, R. and Ahmad, N. (2005). Physiological and biochemical aspects of seed vigour enhancement treatments in fine rice (Oryza sativa L.). Seed Science & Technology, 33: 623-628.
  5. Blum, A. and Ebercon, A., (1981). Cell membrane stability as a measure of drought and heat tolerance in wheat. Crop Science, 21 (1):43-47.
  6. Boonasiri, K., Ketsa, S., Van-doorn W.G., (2007). Seed browning of hot peppers during low temperature storage. Postharvest Biol. Technol., 45: 258 - 365.
  7. Coolbear, P., Slater, R. J. and Bryant, J. A. (1990). Changes in nucleic acid levels associated with improved germination performance of tomato seeds after low temperature pre-sowing treatment. Annals of Botany, 665: 187 - 195.
  8. Dasgupta, P. R. and Austenson, (1973). Analysis of inter relationship among seedling vigour, field emergence and yield in wheat. Agronomy Journal, 65: 417 - 422.
  9. Dubois, K.G., Hamilton, J.A., Rebers, P.A. and Smith, F., (1951). A Colorimetric Method for the determination of Sugars. Nature, 167: 168.
  10. Ellis, R. A. and Roberts, E. H., (1981). The quantification of ageing and survival of orthodox seeds. Seed Science and Technology, 9: 373 - 409.
  11. Evans, L.E. and Bhatt, G.M., (1977). Influence of seed size, protein content and cultivar on early seedling vigour in wheat. Canadian Journal of Plant Science, 57: 929-935.
  12. Fu, J.R., Lu, X.H., Chen, R.Z., Liu, Z.S. and Cai, D.Y., (1988), Osmoconditioning of peanut seeds with PEG to improve vigour and some biochemical activities. Seed Sci. and Technol., 16: 197-212.
  13. Glaszman, J.C., Delos Reyes, B.G. and Khush, G.S., (1988). Electrophoretic variation of isozymes in plumules of rice. IRRI Res. Paper series, 134: 1 - 3.
  14. Goth, L.,(1991), A simple method for determination of serum catalase and revision of reference range. Clin. Chim. Acta, 196: 143 - 152.
  15. Harris, D.; Joshi, A., Khan, P.A., Gothkar, P. and Sodhi, P.S. (1999). On-farm seed priming in semi-arid agriculture: Development and evaluation in maize, rice and chickpea in India using participatory methods. Experimental Agriculture 35: 15 – 29.
  16. Hsu, J.L. and Sung J.M. (1997). Antioxidant role of glutathione associated with accelerated aging and hydration of triploid watermelon seeds. Plant Physiology, 91: 703–707.
  17. ISTA, (2007), International Rules for Seed Testing, Published by International Seed Testing Association, Bassersdorf, Switzerland.
  18. Khajeh-Hosseini, M., Powell A.A., and Bingham I.J., (2003). The interaction between salinitystress and seed vigor during germination of soybean seeds. Seed Science and Technology, 31: 715-725.
  19. Kittock, D. L. and Law, A. G., (1968), Relationship of seedling vigour to respiration and tetrazolium chloride reduction by germinating wheat seeds. Agronomy Journal, 60: 286-288.
  20. Lowry, O. H., Rose Brough, J., Farrland, A.L., and Randall, R. J., (1951). Protein measurement with Folin-Phenol reagent. J. Biol.Chem., 193: 265-275.
  21. Masterson, C., Blackburn, A. and Wood, C., (2000), Acyl – CoA dehydrogenase activity in pea cotyledon tissue during germination and initial growth. Biochemical Society Transaction 28 (6): 760 – 762.
  22. McDonalds, M. B., (1999), Seed deterioration physiology, repair and assessment. Seed Science and Technology, 27: 177 - 237.
  23. Mir Mahmoodi, T., Ghassemi-Golezani, K., Habibi, D., Paknezhad, F., Ardekani M, R., (2011). Effect of hydro-priming duration on seedling vigour and field establishment of maize (Zea mays L.). Research on Crops, 12: 341-345.
  24. Osborne, D.J., (1983), Biochemical control systems operating in the early hours of germination. Indian Journal of Botany, 61: 3568 - 3577.
  25. Powell, A.A., Yule, L.J., Jing, H.C., Groot, S.P.C., Bino, R.J. and Pritchard, H.W., (2000). The influence of hydrated seed treatment on seed longevity as assessed by the viability equation. Journal of Experimental Botany, 51 (353): 2031 - 2043.
  26. Sadasivam, S. and Manickam, A. (2008). Biochemical Methods. 3rd Eein, New Age International Publishers, New Delhi, India. 
  27. Sarika, G. Basavaraju, G.V., Bhanuprakash, K. Chaanakeshva, B. C. Paramesh, R. and Radha, B.N. (2013). Investigations on seed viability and Vigour of aged seeds by Priming in french bean (Phaseolus vulgaris L.) Vegetable Science 40 (2): 169-173.
  28. Serge kibinza, Jernmie Bazin, Christophe Bailly, Jill M.Farrant, Fran Corbineau and Harat El-Maarouf-Bouteau, (2011). Catalase is a key enzyme in recovery from ageing during priming. Plant Science 181: 309–315
  29. Wang, Q. and Sang, L. L., (2008). Study on hard seed and germinating characteristic of Rhus chinensis Mill. Journal of Modern Agriculture, 4: 58 – 61 
Global footprints


© 2015 ARCC JOURNALS. All Rights Reserved. Powered By ARCC JOURNALS