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Legume Research

Diversity analysis of pea genotypes using RAPD markers

DOI: 10.18805/LR-3709    | Article Id: LR-3709 | Page : 196-201
Citation :- Diversity analysis of pea genotypes using RAPD markers.Legume Research.2018.(41):196-201
Bharti Thakur, Susheel Sharma, Isha Sharma, Prachi Sharma, Deepika and Sajad Majeed Zargar drsusheelsharma@rediffmail.com
Address : Division of Plant Pathology, Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu-180 009, Jammu and Kashmir, India.
Submitted Date : 23-03-2016
Accepted Date : 11-03-2017
First Online:

Abstract

Genetic diversity among 54 genotypes of field and garden pea of India were studied using 30 RAPD primers. A total of 168 amplicons were scored, of which 154 were polymorphic revealing 89.3% of polymophism. Out of 30 RAPD primers tested, 22 primers produced clear and reproducible bands. The number of products generated by these primers ranged from 04 to 14 with primer OPU-17 giving maximum (14) and primer OPC-08 giving minimum number of amplicons (04). Genetic similarity estimates based on the binomial data using Jaccard’s coefficient ranged from 0.34 (Kaza-2/IC-218991) to 0.89 (IC-209118/IC-209123) with an average similarity index of 0.54 exhibiting considerable diversity among the pea genotypes studied. The dendrogram showed clear pattern of clustering according to the source of germplasm. This study indicated that Himalayan region germplasm exhibited higher diversity. 

Keywords

Genetic diversity Pisum sativum RAPD.

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