Legume Research

  • Chief EditorJ. S. Sandhu

  • Print ISSN 0250-5371

  • Online ISSN 0976-0571

  • NAAS Rating 6.80

  • SJR 0.391

  • Impact Factor 0.8 (2024)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Legume Research, volume 40 issue 2 (april 2017) : 224-231

Molecular tagging of pod shattering tolerance trait in soybean [Glycine max (L.) Merrill] genotype MACS-450.

Dilip S. Thakare*, Vivek P. Chimote, Amit Adsul, Milind P. Deshmukh, Santosh C. Pulate
1<p>Department of Agricultural Botany,&nbsp;Mahatma Phule Krishi Vidyapeeth, Rahuri-413 722, Maharashtra, India.</p>
Cite article:- Thakare* S. Dilip, Chimote P. Vivek, Adsul Amit, Deshmukh P. Milind, Pulate C. Santosh (2017). Molecular tagging of pod shattering tolerance trait in soybean[Glycine max (L.) Merrill] genotype MACS-450. . Legume Research. 40(2): 224-231. doi: 10.18805/lr.v0i0.7299.

ABSTRACT

Pod shattering (Dehiscence) severely reduces seed yield in soybean. Molecular tagging of pod shattering resistance can help in the process of breeding for shattering tolerance. In this study, an segregating population of cross (susceptible cultivar Monetta x tolerant genotype MACS-450) was used for bulked segregant analysis (BSA) with 26 SSR (simple sequence repeat) primers known to amplify markers linked to 22 qPDH loci. Among them, eight polymorphic SSR markers, viz., Sat_350 (qPDH1-7), Satt185 (qPDH1-3), Satt674 (qPDH1-5 loci), Satt166 (qPDH3-5), SRM1 (qPDH1 loci) Sat_342 (qPDH3-2), Satt685 (qPDH1-2) and Sat_407 (qPDH3-1) were able to distinguish parents differing for pod shattering of them two primers Satt 166 and SRM1 yielded markers polymorphic in between shattering tolerant and susceptible bulks by amplifying only Satt166-200 bp marker and SRM1-234bp marker only in tolerant bulks. Only Satt166-200 bp marker was observed in tolerant parent, bulks and 14 plants; while another 237 bp marker for pod shattering susceptibility got amplified in 46 plants including 8 plants that were heterozygous for both alleles. SRM1-234 bp marker was observed only in tolerant parent, bulks and 15 plants; while another 237 bp marker for pod shattering susceptibility got amplified in 45 plants. In combined marker analysis, the markers Satt 166 (qPDH3-5 loci) and SRM1 (qPDH1 loci) were linked with pod shattering score and were also confirmed in individual 60 F2 segregants. Hence, these markers could be utilized in the marker assisted for pod shattering resistance/tolerance breeding of qPDH3-5 like and qPDH1 genes.

REFERENCES


  1. Anonymous (1979). International Cooperators Guide: Suggested cultural practices for soybean. No. Asian Vegetable Research. Development Center, Taiwan. pp. 79-112.

  2. Carpenter, J.A. and Fehr, W.R. (1986) Genetic variability for desirable agronomic traits in population containing Glycine soja germplasm. Crop Sci., 26: 681-686.

  3. Caviness, C.E. (1963) A physiological and genetic study of shattering in soybean. Ph. D. Dissertation, University of Missouri.

  4. Che, J.Y., Liu, C.Y., Jiang, H.W., Han, X., Mao, Y. Z., Xin, D.W, Chen, Q. S. and Hua, G.H. (2013). Identification of soybean maturity SSSLs and QTL mapping based on meta-analysis. Scientia Agricultura Sinica, 46: 4646-4656.

  5. Collard, B.C.Y., Jahufer, M.Z.Z., Brouwer, J.B. and Pang, E.C.K. (2005) An introduction to markers, quantitative trait loci (QTL) mapping and marker-assisted selection for crop improvement: The basic concepts. Euphytica, 142: 169­196.

  6. Cregan P.B., Jarvik T, Bush A.L., Shoemaker R.C., Lark K.G., Kahler A.L., Kaya N., Van Toai T.T, Lohnaes D.G., Chung T., Specht J.E. (1999) An integrated genetic linkage map of the soybean genome. Crop Sci. 39:1464–1490.

  7. Funatsuki, H., Ishimoto, M., Tsuji, H., Kawaguchi, K., Hajika, M.,Fujino, K. 2006. Simple sequence repeat markers linked to a major QTL controlling pod shattering in soybean. Plant Breeding, 125: 195–197.

  8. Funatsuki, H., Hajika, M., Hagihara, S., Yamada, T., Tanaka, Y., Tsuji, H., Ishimoto, M. and Fujino, K. (2008) Confirmation of the location and the effects of a major QTL controlling pod dehiscence, qPDH1, in soybean. Breeding. Sci., 58: 63-69.

  9. Grant, D., Nelson, R.T., Cannon, S.B. and Shoemaker, R.C. (2010) SoyBase, the USDA-ARS soybean genetics and genomics database. Nucl. Acids Res. 38 (suppl 1): D843-D846. doi: 10.1093/nar/gkp798

  10. Hoeck, J.A., Fehr, W.R., Shoemaker, R.C., Welke, G.A., Johnson, S.L. and Cianzio, S.R. (2003) Molecular marker analysis of seed size in soybean. Crop Sci., 43: 68-74.

  11. Jenkins, E.S., Paul, W., Coupe, S.A., Bell, S.J., Davis, E.C. and Roberts, J.A. (1996) Characterization of an mRNA encoding a polygalacturonase expressed during pod dehiscence in oilseed rape. (Brassica napus L.). J. Exp. Bot., 47: 111-    115.

  12. Kang, S, Kwak, K, Kim, K, Choung, M, Han, W, Baek, I, Kim, M, Van, K, and Lee S (2009) Population-specific QTLs and their different epistatic interactions for pod dehiscence in soybean [Glycine max (L.) Merr.]. Euphytica 166:15­24.

  13. Keim, P., Olson, T.C. and Shoemaker, R.C. (1988) A rapid protocol for isolating soybean DNA. Soybean Genetics Newsletter, 15: 150-152.

  14. Michelmore, R.W., Paran, I. and Kesseli, R.V. (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc. Natl. Acad. Sci. USA., 88: 9828-9832.

  15. Morgan, C.L., Bruce, D.M., Child, R., Ladbrooke, Z.L. and Arthur, A.E. (1998) Genetic variation for pod shatters resistance among lines of oilseed rape developed from synthetic Brassica napus. Field Crops Res., 58: 153 -165.

  16. Nyinyi, C.N., Allen, F.L., Kopsell, D.A., Sams, C.E. and Pantalone, V.R. (2006) 2431 Joe Johnson, Dept. of Plant Science, Uni. of Tennessee, 252.

  17. Suzuki M, Fujino K, Funatsuki H (2009) A major soybean QTL, qPDH1, controls pod dehiscence without marked morphological change. Plant Prod. Sci 12:217–223.

  18. Suzuki, M, Fujino, K, Nakamoto, Y, Ishimoto, M, Funatsuki, H (2010) Fine mapping and development of DNA markers for the qPDH1 locus associated with pod dehiscence in soybean. Mol. Breeding, 25:407-418

  19. Tanksley, S.D., Young, N.D., Paterson, A.H. and Bonierbale, M.W. (1989). RFLP mapping in plant breeding new tools for an old science. Biotech., 7: 257-264.

  20. Tiwari, S.P. and Bhatnagar, P.S. (1997) Screening methods for pod shattering in soybean. In Proceedings. World Soybean Research Conference V, Chiang Mai, Thailand. Kasetsart University Press .pp.23-24.

  21. Tsuchiya, T. (1986). Studies on shattering resistance in soybean Glycine max breeding. Report Hokkaido Precision Agri. Expt. Station., 58:1- 53.

  22. Tukamuhabwa, P., Rubaihayo, P. and Dashiell, K.E. (2002) Genetic components of pod shattering in soybean. Euphytica, 125: 29-34.

  23. Tukamuhabwa, P. (2000) Genetics of resistance to pod shattering in soybean. Ph.D. thesis. Department of Crop Science. Makerere University Kampala.

  24. Yamada, T., Funatsuki, H., Hagihara, S., Fujita, S., Tanaka, Y., Tsuji, H., Ishimoto, M., Fujino K. and Hajika. M. (2009). A major QTL, qPDH1, is commonly involved in shattering resistance of soybean cultivars. Breeding Sci., 59:435-    440.

  25. Yamada, T., Hajika, M., Funatsuki, H., Yamada, N., Takahashi, K. and Ohki, N. (2010) Development of shattering-resistant lines by backcrossing to major soybean cultivars. Breeding Res., 12 (Suppl. 1): 199.
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)