Legume Research

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Research Article

Legume Research, volume 43 issue 2 (april 2020) : 165-171

Characterization and identification of pigeonpea [Cajanus cajan (L.) Millsp.] genotypes based on quality of seed protein

Ashish Kumar Pandey, Ajai Kumar Singh, Prakash Singh, Rajendra Prasad Vyas, Ravish Kumar Singh, Hemraj Bhandari
1Department of Genetics and Plant Breeding,<br />Chandra Shekhar Azad University of Agriculture and Technology, Kanpur-208 002, Uttar Pradesh, India.&nbsp;

  • Submitted02-03-2016|

  • Accepted18-05-2019|

  • First Online 14-08-2019|

  • doi 10.18805/LR-3696

Cite article:- Pandey Kumar Ashish, Singh Kumar Ajai, Singh Prakash, Vyas Prasad Rajendra, Singh Kumar Ravish, Bhandari Hemraj (2019). Characterization and identification of pigeonpea [Cajanus cajan (L.) Millsp.] genotypes based on quality of seed protein. Legume Research. 43(2): 165-171. doi: 10.18805/LR-3696.

ABSTRACT

Tris- and water-soluble seed protein profiling was used to characterize, identify and differentiate 13 genotypes of pigeonpea via SDS–PAGE based electrophoresis of seed protein. Electropherograms, RM-value and UPGMA based cluster (dendrograms) analysis was used to analyze tris- and water-soluble protein banding patterns of these genotypes. It revealed that RM-value of protein bands have differed from all the genotypes for both soluble proteins. Tris-soluble protein banding pattern exhibited 17 bands including 12-15 variate of RM-value (0.083 to 0.98) and two common bands with RM-value 0.083 and 0.78. However, water-soluble protein banding pattern revealed the presence of 16 bands including 8-12 variate of RM-value (0.16 to 0.95) with four common bands of RM-value 0.43, 048, 0.23 and 0.65, respectively. UPGMA cluster analysis was used to group the 13 genotypes into six distinct clusters. The banding pattern of tris-soluble protein was found to be more distinct than water-soluble protein. The result demonstrated that the electrophoretic profile of tris-soluble protein through SDS-PAGE was more effective than the water-soluble protein.

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