Characterization and identification of pigeonpea [Cajanus cajan (L.) Millsp.] genotypes based on quality of seed protein

DOI: 10.18805/LR-3696    | Article Id: LR-3696 | Page : 165-171
Citation :- Characterization and identification of pigeonpea [Cajanus cajan (L.) Millsp.] genotypes based on quality of seed protein.Legume Research-An International Journal.2020.(43):165-171
Ashish Kumar Pandey, Ajai Kumar Singh, Prakash Singh, Rajendra Prasad Vyas, Ravish Kumar Singh and Hemraj Bhandari prakash201288@gmail.com
Address : Department of Genetics and Plant Breeding,
Chandra Shekhar Azad University of Agriculture and Technology, Kanpur-208 002, Uttar Pradesh, India. 
Submitted Date : 2-03-2016
Accepted Date : 18-05-2019

Abstract

Tris- and water-soluble seed protein profiling was used to characterize, identify and differentiate 13 genotypes of pigeonpea via SDS–PAGE based electrophoresis of seed protein. Electropherograms, RM-value and UPGMA based cluster (dendrograms) analysis was used to analyze tris- and water-soluble protein banding patterns of these genotypes. It revealed that RM-value of protein bands have differed from all the genotypes for both soluble proteins. Tris-soluble protein banding pattern exhibited 17 bands including 12-15 variate of RM-value (0.083 to 0.98) and two common bands with RM-value 0.083 and 0.78. However, water-soluble protein banding pattern revealed the presence of 16 bands including 8-12 variate of RM-value (0.16 to 0.95) with four common bands of RM-value 0.43, 048, 0.23 and 0.65, respectively. UPGMA cluster analysis was used to group the 13 genotypes into six distinct clusters. The banding pattern of tris-soluble protein was found to be more distinct than water-soluble protein. The result demonstrated that the electrophoretic profile of tris-soluble protein through SDS-PAGE was more effective than the water-soluble protein.

Keywords

Pigeonpea genotype SDS–PAGE Varietal identification Tris- and water-soluble protein.

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