Chief EditorJ. S. Sandhu
Print ISSN 0250-5371
Online ISSN 0976-0571
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Grain protein estimation and SDS-PAGE profiling of six important arid legumes
First Online 03-03-2017|
Grain protein content and protein profiles of six arid grain legumes belonging to three different tribes were studied. Average grain protein (per cent) of horse gram, cowpea, moth bean, guar, chickpea and green gram was 20.29, 22.8, 22.6, 20.6, 21.82, and 20.40 respectively. All the six arid legumes were clearly differentiated based on the protein banding patterns generated through SDS-PAGE. The molecular weight range of proteins in different legumes was observed to be ~16 to ~250kD. Interestingly, in chickpea, moth bean and horse gram proteins >100kD were not mostly observed. Further, distinct proteins were observed indicating inter-varietal differences in horse gram (CRHG-19), cowpea (RC-101) and guar (HG-2-20), which could be useful in varietal identification. The different legumes were broadly clustered in to four clusters. Further, chickpea (Cicereae), green gram and cowpea (Phaseoleae) were clustered together. The similarity indexes of varieties and individual crops ranged from 0.667 to 0.950 and 0.425 to 0.821 respectively. Though the six legumes belong to Leguminosae they have distinct protein profiles which could be useful in species/varietal identification.
- Adekola, O.F. and Oluleye, F. (2007). Influence of mutation induction on the chemical composition of cowpea [Vigna unguiculata (L.) Walp]. Afr. J. Biotechnol., 6:2143-2146.
- Atta, S., Maltese, S. and Cousin, R. (2004). Protein content and dry weight of seeds from various pea genotypes. Agronomie, 24:257-266.
- Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Ann. Biochem., 72:248-254.
- Burstin, J., Gallardo, K., Mir, R.R., Varshney, R.K. and Duc, G. (2011). Improving protein content and nutrition quality. In: Biology and Breeding of Food Legumes. CAB International, Wallingford, UK, 314-328.
- Cereletti, P. (1979). The legume proteins. Proceedings of Congress of PPI. Perugia, Italy, 30 May -2 June, 31-57.
- Christou, P. (1994). The biotechnology of crop legumes. Euphytica, 74:165-185.
- Crochemore, M.L., Huyghe, C., Papineau, J. and Julier, B. (1994). Intra-plant variability in seed size and seed quality in Lupinus albus L. Agronomie, 14:5-13.
- Erskine, W. and Muehlbauer, F.J. (1991). Allozyme and morphological variability, outcrossing rate and core collection formation in lentil germplasm. Theor. Appl. Genet., 83:119-125.
- Frimpong, A., Sinha, A., Tar’an, B., Warkentin, T.D., Gossen, B.D. and Chibbar, R.N. (2009). Genotype and growing environment influence chickpea (Cicer arietinum L.) seed composition. J. Sci. Food Agric., 89:2052-2063.
- Garcia-Vallvé, S., Palau, J. and Romeu, A. (1999). Horizontal gene transfer in glycosyl hydrolases inferred from codon usage in Escherichia coli and Bacillus subtilis. Mol. Biol. Evol., 16:1125-1134.
- Gueguen, J. and Barbot, J. (1988). Quantitative and qualitative variability of pea (Pisum sativum L.) protein composition. J. Sci. Food Agric., 42:209-224.
- Hedley, C.L. (2001). Introduction. In: Carbohydrate in grain legume seeds (Ed. C.L. Hedley). 1-11.
- Iqbal, S.H., Ghafoor, A. and Ayub, N. (2005). Relationship between SDS-PAGE markers and Ascochyta blight in chickpea. Pak. J. Bot., 37:87-96.
- Javaid, A., Ghafoor, A. and Anwar, R. (2004). Seed storage protein electrophoresis in groundnut for evaluating genetic diversity. Pak. J. Bot., 36:25-29.
- Karihaloo, J.L., Kaur, M. and Singh, S. (2002). Seed protein diversity in Solanum melongena L., and its wild and weedy relatives. Gen. Res. Crop Evol., 49:533-539.
- Ladizinsky, G. and Van Oss, H. (1984). Genetic relationships between wild and cultivated Vicia ervilia L. Bot. J. of Linn. Soc., 89:97-100.
- Ladizinsky, G. and Hymowitz, T. (1979). Seed protein electrophoresis in taxonomic and evolutionary studies. Theor. Appl. Genet., 51:145-151.
- Lewis, G., Schrire, B., MacKinder, B. and Lock, M. (2005). Legumes of the world. Royal Botanic Gardens, Kew, UK.
- Michener, C. and Sokal, R. (1957). A quantitative approach to a problem in classification. Evolution, 11:130-162.
- Oluwatosin, O.B. (1997). Genetic and environemental variation for seed yield, protein, lipid and amino acid composition in cowpea [Vigna unguiculata (L) Walp]. J. Sci. Food Agric., 74:107-116.
- Omonhinmin, C.A. and Ogunbodede, O.O. (2013). Genetic diversity, taxonomy and legumins implications of seed storage protein profiling in Fabaceae. Afr. J. Biotechnol., 12:2157-2163.
- Polhill, R.M. (1994). Classification of the Leguminosae. In: Phytochemical dictionary of the Leguminosae (Eds. F.A., Bisby, J. Buckingham, J.B., Harborne). Chapman and Hall, New York, USA, xxxv–xlviii.
- Polhill, R.M. and Raven, P.H. (1981). Advances in legume systematics, parts 1 and 2. Royal Botanic Gardens, Kew, UK.
- Rundel, P.W. (1989). Ecological success in relation to plant form and function in the woody legumes. In: Advances in legume biology. CH, Stirton, JL, Zarucchi,). Monog. Syst. Bot. Missou. Bot. Gard., 29:377-398
- Salmanowicz, B.P. and Przybylska, J. (1992). Seed albumins from some species of Lathyrus, Lens and Cicer genera: comparative analysis by gel filtration and electrophoresis. Genetica Polonica, 33:107-113.
- Saxena, K.B., Kumar, R.V. and Rao, P.V. (2002). Pigeonpea nutrition and its improvement. In: Quality Improvement in Field Crops (Eds. A.S. Basra and I.S. Randhawa.). Food Products Press, 227–260.
- Shaheen, S., Harun, N., Khan, F., Hussain, R.A., Ramzan, S., Rani, S., Khalid, Z., Ahmad, M. and Zafar, M. (2012). Comparative nutritional analysis between Vigna radiate and Vigna mungo of Pakistan. Afr. J. Biotechnol., 11:6694-6702.
- Sprent, J.I. (2001). Nodulation in legumes. Royal Botanic Gardens, Kew, UK.
- Sprent, J.I. and McKey, D. (1994). Advances in legume systematics, Part 5, The nitrogen factor. Royal Botanic Gardens, Kew, UK.
- Sreerama Y.N., Sashikala, V.B., Pratape, V.M. and Singh, V. (2012). Nutrients and antinutrients in cowpea and horse gram ûours in comparison to chickpea ûour: evaluation of their ûour functionality. Food Chem., 131:462–46.
- Summerfield, R.J. and Roberts, E.H. (1985). “Grain Legume Crops”. Collins Pub. London, UK.
- Valizadeh, M. (2001). Seed storage protein profile of grain legumes grown in Iran, using SDS-PAGE. J. Agric. Sci. Technol., 3:287-292.
- Yousaf, Z., Masood, S., Shinwari, Z.K., Khan, M.A. and Rabani, A. (2008). Evaluation of taxonomic status of medicinal species of the genus Hyoscyamous, Withania, Atropa and Datura based on polyacrylamide gel electrophoresis. Pak. J. Bot., 40:229.39-2297.
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