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Temperature induction response and accumulation of starch granules as indices to identify the thermotolerance of pulses at early growth stages
 

DOI: 10.18805/lr.v0i0.8403    | Article Id: LR-3623 | Page : 655-659
Citation :- Temperature induction response and accumulation of starch granules as indices to identify the thermotolerance of pulses at early growth stages .Legume Research.2017.(40):655-659

C. Partheeban, H. Vijayaraghavan, S. Sowmyapriya, S. Srividhya and D. Vijayalakshmi

vijiphysiology@gmail.com
Address :

Department of Crop Physiology, Tamil Nadu Agricultural University, Coimbatore- 641 003, India

Submitted Date : 28-09-2015
Accepted Date : 8-02-2016

Abstract

In pulses, high temperature is a major limiting factor for yield decline. Tamil Nadu has a rich pulse germplasm with high genetic variability. Hence, the present study was designed to develop a technique to screen genotypes for high temperature tolerance. A rapid screening protocol was developed based on the principle of “acquired tolerance”. Adapting this temperature induction response (TIR) technique to pulses, popular varieties of TNAU pulses of blackgram (VBG-07-001, VBG-08-003), redgram (CO-6, VBN-2) and soybean (CO-1, CO-2) were screened for thermotolerance. The challenging lethal temperature was standardized as 50°C for 3 hours at which 90 per cent of the seedling mortality occured. The induction temperature was standardized as 36°C to 40°C at which 48.3 per cent of growth reduction over control was noticed. Blackgram showed higher thermotolerance than other pulses in terms of survival percentage and seedling growth. Blackgram seeds showing contrasting behaviour to temperature stress tolerance namely VBG-07-001(tolerant) and VBG-08-003 (susceptible) were analysed for the accumulation and distribution of starch granules responsible for the grain filling under heat stress.

Keywords

Lethal temperature Pulses Scanning Electron Microscope (SEM) Starch granules Temperature Induction Response (TIR) Thermotolerance.

References

  1. Alagu PalamuthirSolai, M. and Datta. D. (2014). Evaluation of Pigeon Pea [Cajanuscajan (L.] Millsp.)Genotypes for Thermotolerance Tolerance Based on Temperature Induction Response (TIR) technique.Trends in Bioscience.7(23):3914-3919
  2. Ali, M., Gupta S. and Basu P.S. (2009). Higher level of warming in north India will affect crop productivity. Hindu Survey of Indian Agriculture.p.44–49.
  3. Barnabas B, Jager K and Feher A. (2008).The effect of drought and heat stress on reproductive processes in cereals.Plant Cell and Environment.31: 11-38.
  4. Bodenhuizen, N. P. (1969). The biogenesis of starch granules in higher plants, Appl-Eton Crofts, New York.
  5. Burke, J.J. (1994). Integration of acquired thermotolerance within the developmental program of seed reserve mobilization.. In [J.H. Cherry (ed.)] Biochemical and Cellular Mechanisms of Stress Tolerance in Plants.Springer,Berlin. p. 191–200
  6. Burke, J.J., (1998). Characterization of acquired thermotolerance in soybean seedlings.Plant Physiol. Biochem. 36:601–607.
  7. Fender, S.E. and O’Connell M.A. (1990). Expression of the heat shock response in a tomato interspecific hybrid is not intermediate between the two parental responses. Plant Physiol. 93: 1140 - 1146.
  8. Gandhi, D., Albert S. and Pandya N.(2011). Morphological and micromorphological characterization of some legume seeds from Gujarat, India. Environ. Exp. Biol.9: 105–113.
  9. Geingenberger, P. (2011). Regulation of starch biosynthesis in response to a fluctuating environment. Plant Physiol., 155(4):1566-1577.
  10. Hikosaka, K., Ishikawa K., Borjigidai A., Muller O. and Onoda Y. (2006). Temperature acclimation of photosynthesis: mechanisms involved in the changes in temperature dependence of photosynthetic rate. J. Exp. Bot.57: 291-302.
  11. Howarth, C.J. and Skot K.P. (1994). Detailed characterization of heat shock proteins synthesis and induced thermotolerance in seedlings of Sorghum bicolor L. J. Exp. Bot.45: 1353-1363.
  12. IPCC. (2007). Climate Change 2007: The Physical Science Basis. Contribution of Working Group I to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change. In: Solomon, S., D. Qin, M. Manning, Z. Chen, M. Marquis, K.B. Averyt, M. Tignor and H.L. Miller (Eds). Cambridge University Press, Cambridge, United Kingdom and New York, NY, USA, pp. 996.
  13. Keeler, S.J., Boettger C.M., Haynes J.G., Kuches K.A., Johnson M.M., and Thureen D.L (2000). Acquired thermotolerance and expression of the HSP100/ClpB genes of lima bean.Plant Physiol.123:1121–1132.
  14. Kumar, G., Krishna B.T., Savitha M., Gopalakrishna R., Mukopadhyay K., Ram G. Mohan and Udayakumar M. (1999). Enhanced expression of heat shock proteins in thermo tolerant lines from the progenies selected on the basis of temperature induction response (TIR). Theor. Appl. Genet. 99: 359-367.
  15. Larkindale, J., Hall J.D., Knight M.R. and. Vierling E. (2005). Heat stress phenotypes of Arabidopsis mutants implicate multiple signaling pathways in the acquisition of thermotolerance. Plant Physiol. 138: 882-97.
  16. Massie, M.R.,. Lapoczka E.M, Boggs K.D., Stine K.E., and White G.E. (2003). Exposure to the metabolic inhibitor sodium azide induces stress protein expression and thermotolerance in the nematode Caenorhabditiselegans. Cell Stress Chaperones 8:1–7.
  17. Smirnova J, Fernie AR, Steup M (2015) Chapter 7. Starch Degradation. In: Starch: Metabolism and Structure (Nakamura Y, ed.). Springer, ISBN 978-4-431-55494-3 pg. 239-290.
  18. Senthil Kumar, M. and Udayakumar M.(2004). Development of thermotolerant tomato (Lycopersicumesculentum Mill.) lines: an approach based on mutagenesis. J. Plant Biol. 31: 139-48.
  19. Senthil Kumar, M., Kumar G.,. SrikanthBabu V and Udayakumar M. (2006). Assessment of variability in acquired tolerance: Potential option to study genotypic responses and the relevance of stress genes. J. Plant Physiol. 164(2):111-25.
  20. Srikanthbabu, V., Ganesh Kumar, Krishnaprasad B. T., Gopalakrishnan R., Savitha M. and Udayakumar M.(2002). Identification of pea genotypes with enhanced thermotolerance using temperature induction response (TIR). J. Plant Physiol. 159: 535-545.
  21. Yan SH, Yin YP, Li WY, Li Y Liang ,T B Wu, Geng YH and Wang QH ZL (2008). Effect of high temperature after anthesis on starch formation of two wheat cultivars differing in heat tolerance.Acta Ecologica Sinica. 28 (12): 6138-6147.
  22. Yude, C., H. Kaiwei, L. Fuji and Y. Jie. 1993. The potential and utilization prospects of kinds of wood fodder resources in Yunnan. Forest. Res. 6: 346-350. 

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