Chief EditorJ. S. Sandhu
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Coat protein and movement protein based characterization of Lima bean yellow mosaic virus disease in Karnataka (India)
First Online 30-03-2017|
Yellow mosaic disease caused by a Begomovirus has become an important production constraint in lima bean cultivation in Karnataka. The begomovirus causes Lima bean Yellow Mosaic Virus (LYMV), was characterized by sequencing the CP and MP genes. Distinct viral gene specific PCR product corresponding to CP and MP gene ~1000 bp and ~900 bp was obtained. The PCR product were sequenced and compared with the reference Begomoviruses sequences from the NCBI blast database. LYMV (GKVK isolate) shared highest nucleotide identity (98%) with Horsegram yellow mosaic virus (HgYMV) of French bean isolate (Accession No. AM932425.1). Phylogenetic analysis of CP and MP gene sequences of LYMV-GKVK isolate showed that LYMV-GKVK clustered with isolates of HgYMV Horsegram and French bean isolates of Tamil Nadu, Srilanka, Karnataka. Based on Nucleotide Identity begomovirus infecting lima bean at Bangalore is to be considered as strain of HgYMV. The disease was also successfully transmitted to French bean and Horsegram when experimentally transmitted using whitefly vector. Therefore, the begomovirus causing yellow mosaic disease of lima bean is considered as strain of HgYMV. Lima bean is infected by Mungbean Yellow Mosaic India Virus (MYMIV) according to earlier reports whereas our study the reports the infection of HgYMV on Lima bean from Karnataka. Thus it is concluded that Lima bean is infected by both MYMIV and HgYMV in different geographical locations.
- Agbagwa, I.O., Datta, P.G., Patil, Singh, P. and Nadarajan, N. (2012). A protocol for high-quality genomic DNA extraction from legumes. Genet. Mol. Res., 11 : 4632-4639.
- Asurmendi, S., Berg, R.H, Koo, J.C. and Beachy, R.N. (2004). Coat protein regulates formation of replication complexes during tobacco mosaic virus infection. Proc. Natl. Acad. Sci.(USA) 101: 1415 1420.
- Borah, B.K. and Dasgupta, I. (2012). Begomovirus research in India: A critical appraisal and the way ahead. J. Biosci., 37: 791–806.
- Fauquet, C. M., Bisaro, D. M., Briddon, R. W., Brown, J. K., Harrison, B. D., Rybicki, E. P., Stenger, D. C. and Stanley, J. (2003). Revision of taxonomic criteria for species demarcation in the family Geminiviridae and an updated list of begomovirus species. Arch. Virol., 148: 405-421.
- Fofana, B., Baudoin, J.P., Vekemans, X., Debouck, D.G. and Du Jardin, P. (1999). Molecular evidence for an Andean origin and a secondary gene pool for the Lima bean (Phaseolus lunatus L.) using chloroplast DNA. Theoretical Applied Genetics, 98: 202-212.
- Harrison, B. D., Swanson, M. M. and Fargette, D. (2002). Begomovirus coat protein: serololy, variation and functions. Physiol. Mol. Pl. Pathol., 60: 257-271.
- Hussain, M., Qazi, J., Mansoor, S., Iram, S., Bashi, M. and Zafar, Y. (2004). First report of Mungbean yellow mosaic India virus on mungbean in Pakistan. Pl.Pathol., 53: 518.
- Qazi, J., Mansoora, S., Amina, I., Awanb, M.Y, Briddona, R. W. and Zafar, Y. (2006). First report of Mungbean yellow mosaic virus on moth bean in Pakistan. Pl.Pathol., 55: 818.
- Galvez, G. E. and Cardenas, M. (1980). Whitefly transmitted viruses. In :Bean production problem (Eds. H. F. Schwartz and G. E. Galvez), pp261-289, Centro International de Agricultural Tropical, Cali, Colombia.
- Govindu, H.C., (1964). A review on virus diseases of crop plants. Information pamphlet No.2 (Research series). Directorate of Agriculture, Bangalore. 13 pp.
- Maruthi, M. N., Colvin, J., Seal, S.E., Gibson, G. and Cooper, J. (2002). Co-adaptation between cassava mosaic geminiviruses and their local vector populations. Virus Res., 86: 71-85.
- Mc Grath, P. F. and Harrison, B. R . (1995). Transmission of tomato leaf curl geminivirus by Bemisia tabaci, effects of virus isolate and vector biotype. Ann. Appl. Biol., 126: 307-316.
- Naimuddin and Mohd. Akram. (2010). Detection of mixed infection of begomoviruses in cowpea and their molecular characterization based on CP gene sequences. J. Food Legumes, 23: 191-195.
- Obaiah, S., Bhaskara Reddy, B.V., Eswara Reddy, N.P. and Siva Prasad, Y. (2013). Molecular detection of yellow mosaic virus infecting blackgram [Vigna mungo (L.) hepper] in Andhra pradesh. Int. J. Pl. Ani. Envi. Sci., 4.
- Prins, M., Laimer, M., Noris, E., Schubert, J., Wassenegger, M. and Tepfer, M. (2009). Strategies for antiviral resistance in transgenic plants. Mol. Plant Pathol., 9: 73 83.
- Qu, F., Ren, T. and Morris, T.J. (2003). The coat protein of turnip crinkle virus suppresses post transcriptional gene silencing at an early initiation step. J. Virol., 77: 511 522.
- Wyatt, S. D. and Brown, J. K. (1996). Detection of subgroup III geminivirus isolates in leaf extracts by degenerate primers. Phytopathol., 86: 1288-1293.
- Reichert, V.L., Choi., M., Petrillo, J.E. and Gehrke, L. (2007). Alfalfa mosaic virus coat protein bridges RNA and RNA dependent RNA polymerase in vitro. Virology, 364: 214 226.
- Richa Maheshwari, Gatikrushna Panigrahi. and Angappan, K. (2014). Molecular characterization of distinct YMV (Yellow mosaic virus) isolates with the aid of coat protein gene as amarker for identification. Molecular biology reports, 41:2635-2644.
- Rouhibakhsh, A., Priya, J., Periasamy, A., Haq, Q. M. I. and Malathi, V. G. (2008). An improved DNA isolation method and PCR protocol for efficient detection of multicomponents of begomovirus in legumes. J. Virological Methods, 147: 37- 42.
- Shahid, M. S., Ikegami, M. and Natsuaki, K. T. (2012). First report of Mungbean yellow mosaic India virus on Lima bean affected by yellow mosaic disease in Nepal. Australasian Plant Dis. Notes, 7: 85-89.
- Tamura K., Glen Stecher, Daniel Peterson, Alan Filipski. and Sudhir Kumar. (2013), MEGA6:Molecular genetics analysis version software 6.06. Mol. Biol. Evol., 30:2725–2729.
- Venkataravanappa, V., Swarnalatha, P., Lakshminarayana Reddy, C.N., Mahesh, B. and Rai, A.B. (2012). Molecular Evidence for Association of Tobacco Curly Shoot Virus and a Betasatellite with Curly Shoot Disease of Common Bean (Phaseolus vulgaris L.) from India. J Plant Pathol. Microb., 3:148.
- Zhou, Y.C., Garrido-Ramirez, E. R., Sudarshana, M. R., Yendluri, S. and Gilbertson, R. L. (2007). The N-terminus of the Begomovirus Nuclear Shuttle Protein (BV1) Determines Virulence or Avirulence in Phaseolus vulgaris. MPMI, 20:1523–1534.
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