Legume Research

  • Chief EditorJ. S. Sandhu

  • Print ISSN 0250-5371

  • Online ISSN 0976-0571

  • NAAS Rating 6.80

  • SJR 0.391

  • Impact Factor 0.8 (2023)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Legume Research, volume 39 issue 6 (december 2016) : 1013-1020

Enhancing resistance of rice bean to diseases by seed treatment with Pseudomonas flourescens and Bacillus species

Ankush Gupta*, Deepa Khulbe1, P. Srinivas2
1<p>Department of Seed Science and Technology,&nbsp;G.B.P.U.A &amp; T, Pantnagar-263 145, India.</p>
Cite article:- Gupta* Ankush, Khulbe1 Deepa, Srinivas2 P. (2016). Enhancing resistance of rice bean to diseases by seed treatmentwith Pseudomonas flourescens and Bacillus species . Legume Research. 39(6): 1013-1020. doi: 10.18805/lr.v0iOF.10284.

Efficacy of seed bacterization with six rhizobacterial isolates was assessed in laboratory conditions to evaluate their resistance inducing ability in rice bean. Pseudomonas. fluorescens isolates B5, B6, B8, B9 and B17 and Bacillus species isolate B18 were found effective in reducing the percentage of diseased seedling but showed the plant growth promotion. Increased levels of catalase, phenylalanine ammonia lyase (PAL) and poly phenol oxidase (PPO) were observed in seedlings indicating higher levels of ISR. Maximum induction of defense enzymes was observed with isolate B5 followed by B6 and B18. The utilization of indigenous PGPR strains as inducers of plant defense response may offer a practical solution for eco-friendly management of plant diseases. 

  1. Abdul-Baki, A.A. and Anderson, J.D. (1973). Vigour determination in soybean seeds by multiple criteria. Crop Science., 13: 630-632.

  2. Ahemad, M. and Kibret, M. (2014). Mechanisms and applications of plant growth promoting rhizobacteria: Current perspective. Journal of King Saud University – Science., 26: 1–20.

  3. Albert, F. and Anderson, A. J. (1987). The effect of Pseudomonas putida colonization on root surface peroxidases. Plant Physiology., 85: 535-540.

  4. Anonymous (AOSA). (1993). Rules for testing seeds. Journal Seed Technology., 4: 11a-11d.

  5. Ausable, F.M. (1989). Current Protocols in Molecular Biology. Wiley press, N.Y.

  6. Benhamou, N., Klopper, J.W., Quadi-Hallmann, A. and Tuzun, S. (1996). Induction of defense related ultra structural modifications in pea root tissues inoculated with endophytic bacteria. Plant Physiology., 112: 919-929.

  7. Berger, F., Li, H., White, D., Frazer, R.and Leifert, C. (1996). Effect of pathogen inoculum, antagonist density, and plant species on biological control of Phytophtora and Pythium damping-off by Bacillus subtilis Cot1 in high-humidity fogging glasshouses. Phytopathology., 86: 428-433.

  8. Blilou, I., Bueno, P., Ocampo, J. A., and Garcia-Garrido, J. M. (2000). Induction of catalase and ascorbate peroxidase activities in tobacco roots inoculated with the arbuscular mycorrhizal Glomus mosseae. Mycological Research., 104:722-725.

  9. Butt, V. S. (1980). Direct oxidases and related enzymes, In [Davies, D.D. (Ed.)] Comprehensive Treatise. New York, Academic Press, pp. 81-123.

  10. Chelikani, P., Fita, I., and Loewen, P. C. (2004). Diversity of structures and properties among catalases. Cellular and Molecular Life Sciences., 61:192-208.

  11. Chen, C., Belanger, R. R., Benhamou, N. and Paulitz, T. C. (1998). Induced systemic resistance by Pseudomonas spp. impairs pre- and post-infection development of Pythium aphanidermatum on cucumber roots. European Journal of Plant Pathology., 104:877 – 886.

  12. Chen, C., Belanger, R.R., Benhamou, N. and Paulitz, T.C. (2000). Defense enzymes induced in cucumber roots by treatment with plant growth promoting rhizobacteria (PGPR) and Pythium aphanidermatum. Physiological and Molecular Plant Pathology., 56: 13–23.

  13. De Meyer, G. and Hofte, M. (1999). Salicyclic acid produced by the rhizobacterium Pseudomonas aeruginosa 7NSK2 induces resistance to leaf infection by Botrytis cinerea on Bean. Phytopathology., 87: 588-593.

  14. Dorey, S., Baillieul, F., Saindrenan, P., Fritig, B., and Kauffmann, S. (1998). Tobacco class I and class II catalases are differentially expressed during elicitor-induced hypersensitive cell death and localized acquired resistance. Molecular Plant-Microbe Interactions., 11:1102-1109.

  15. Handelsman, J., Raffel, S., Mester, E.H., Wunderlich, L. and Grau, C.R. (1990). Biological control of damping-off of alfalfa seedlings with Bacillus cereus UW85. Applied and Environmental Microbiology., 56: 713-718.

  16. Helbig, J., Trierweiler, B., Schulz, F.A. and Tauscher, B. (1998). Inhibition of Botrytis cinerea and Penicillium digitatum Sacc. by Bacillus sp. (Isolate 17141) in vitro. Journal for Plant Diseases and Plant Protection., 105: 8-16.

  17. ISTA. (2004). International rules for seed testing. Ed. (2005). International Seed Testing Association, bassersdorf, Switzerland.

  18. King, F.O.,Ward, M.K. and Raney, D.E. (1954). Two simple media for the demonstration of pyocyanin and fluorescein. Journal of Laboratory Clinical Medicine., 44: 302-307.

  19. Kosuge, T. (1966). Role of pheolic substances in resistance of plants pathogens. Annual Review of Phytopathology., 7:195-222.

  20. Krebs, B., Hoding, B., Kubart, S., Workie, M.A., Junge, H., Schmiedeknecht, G., Grosch, R., Bochow, H. and Hevesi, M. (1998). Use of Bacillus subtilis as biocontrol agent. I. Activities and characterization of Bacillus subtilis strains. Journal for Plant Diseases and Plant Protection., 105: 181-197.

  21. Leatham, G.F., King, M. and Stahmann, M.A. (1980). In vitro protein polymerization by quinones or free radicals generated by plant or fungal oxidative enzymes. Phytopathology., 70: 1134-1140.

  22. Luck, H. (1974). Catalase in [Bermeyer (Ed.)] In: Methods in Enzymatic Analysis. New York, Academic Press, pp.285. 

  23. Panse, V. C. and Sukhatme, P. V. (1995). Statistical Methods for Agricultural Workers. ICAR, New Delhi.

  24. Podile, R. and Laxmi, V. D. V. (1998). Seed bacterization with Bacillus subtilis, AF1 increases PAL and reduces the incidence of Fusarium wilt in pigeon pea. Journal of Plant Pathology., 146: 255-259.

  25. Ramamoorthy, V., Raguchander, T. and Samiyappan, R. (2002). Induction of defense-related proteins in tomato roots treated with Pseudomonas fluorescens Pf1 and Fusarium oxysporum f. sp. Lycopersi. Plant and Soil., 239: 55–68.

  26. Robb, A.D., Mapson, L.W. and Swain, T. (1964). Activation of the latent lyosinase of broad bean. Nature., 201: 503-504.

  27. Tomiyama, K. (1963). Physiology and Biochemistry of disease resistance of plants. Annual Review of Phytopathology., 1: 295-324.

  28. Van Loon, L. C. (1997). Induced resistance in plants and the role of pathogenesis related proteins. European Journal of Plant Pathology., 103: 753–765. 

Editorial Board

View all (0)