Legume Research

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Research Article

Legume Research, volume 39 issue 5 (october 2016) : 690-697

Genetic diversity analysis in different genotypes of black gram [Vigna mungo (L.) Hepper] using RAPD marker

Divya Vyas*, Arunabh Joshi, Ganesh Rajamani, Devendra Jain, Gunnjeet Kaur
1<p>Department of Molecular Biology and Biotechnology,&nbsp;Rajasthan College of Agriculture, (M.P.U.A.T.), Udaipur &ndash; 313 001, India.</p>
Cite article:- Vyas* Divya, Joshi Arunabh, Rajamani Ganesh, Jain Devendra, Kaur Gunnjeet (2016). Genetic diversity analysis in different genotypes of black gram[Vigna mungo (L.) Hepper] using RAPD marker . Legume Research. 39(5): 690-697. doi: 10.18805/lr.v0iOF.9430.

ABSTRACT

Amongst the various DNA fingerprinting methodologies, randomly amplified polymorphic DNA (RAPD) was used to estimate genetic diversity and relationship amongst 22 black gram genotypes. A total of 25 randomly selected decamers were screened, out of which only 16 got amplified. A total of 133 amplified bands were obtained, out of which 120 were polymorphic. The average percentage of polymorphism was 90.23. The total number of amplified bands varied between 3 (primer OPK-03) and 15 (primer OPC-08) with an average of 9 bands per primer. The overall size of PCR amplified products ranged between 200 bp to 2600 bp. The average PIC was 0.30 ranging from 0.17 to 0.43. Five unique bands (ranging from 200-1200 bp) were detected in four genotypes using 5 RAPD primers. Jaccard’s similarity coefficient values for RAPD primers ranged from 0.58-0.85 with an average of 0.71. Based on dendrogram generated through UPGMA method and PCA, most of the genotypes got divided into three main clusters. Genotypes U-17 and STY-2289 were lying close and thus showed minimum genetic distance while genotypes UH-177 and IPU99-233 had minimum similarity value of 0.42, thus showing maximum divergence. Thus, these results could be used to assess other black gram accessions in the Vigna germplasm pool that can provide useful information towards molecular classification and the genetic marker assisted breeding for crop improvement.

REFERENCES


  1. Abd El-Hady, E.A.A., Haiba, A.A.A., Abd El-Hamid, N.R., Al-Ansary, A.M.F. and Mohamed, A.Y. (2010). Assessment of Genetic Variations in Some Vigna Species by RAPD and ISSR Analysis. New York Science Journal, 3: 120-128.

  2. Das, S. and Mukherjee, K.K. (1995). Comparative study on seed protein of Ipomoea. Seed Science and Technology, 23: 501-509.

  3. De Vries, I. M. (1996). Characterization and identification of Lactuca sativa cultivars and wild relatives with SD Selectrophoresis (Lactuca sect. Lactuca, Compositae). Genetic Resource and Crop Evolution, 43: 193-202.

  4. Doyle, J.J. and Doyle, J.L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf material. Phytochemical Bulletin, 19: 11-15.

  5. Hadrys, H., Balick, M. and Schierwater, B. (1992). Applications of random amplified polymorphic DNA (RAPD) in molecular ecology. Molecular Ecology, 1: 55-63.

  6. Jaccard, P. (1908). Nouvelles researches surla distribution florale. Societe Vaudoise des Science Natulelles Bulletin, 44: 233-170.

  7. Kaga, A., Tomooka, N., Egawa, Y., Hosaka, K. and Kamijima, O. (1996). Species relationships in the subgenus ceratotropis (genus Vigna) as revealed by RAPD analysis. Euphytica, 88: 17-24.

  8. Kannaiyan, S. (1999). Bioresources Technology for Sustainable Agriculture. Associated Publishing Company, New Delhi, pp. 422.

  9. Karuppanapandian, T., Sinha, P.B., Kamarul, H.A. and Manoharan, K. (2006a). Differential antioxidative responses of ascorbate-glutathione cycle enzymes and metabolites to chromium stress in green gram [Vigna radiata (L.) Wilczek] leaves. Journal of Plant Biology, 49: 440-447. 

  10. Karuppanapandian, T., Sinha, P.B., Kamarul, H.A., Premkumar, G. and Manoharan, K. (2006b). Aluminium-induced changes in antioxidative enzyme activities, hydrogen peroxide content and cell wall peroxidase activity in green gram (Vigna radiata L. cv. Wilczek) roots. Journal of Plant Biology, 34: 79-85. 

  11. Karuppanapandian, T., Karuppudurai, T., Sinha, P.B. and Haniya, A.K. (2007). Random Amplified Polymorphic DNA Markers Variability and Relationships Among Black Gram [Vigna mungo (L.) Hepper] Landraces. Journal of Plant Biology, 34: 79-85. 

  12. Karuppanapandian, T., Wang, H.W., Karuppudurai, T., Rajendhran, J., Kwon, M., Jang, C.S., Kim, S.H., Manoharan, K. and Kim, W. (2010). Efficiency of RAPD and ISSR markers in assessing genetic diversity and relationships in black gram (Vigna mungo L. Hepper) varieties. Canadian Journal of Plant Science, 90: 443-452.

  13. Lakshanpaul, S., Chadha, S. and Bhat, K.V. (2000). Random amplified polymorphic DNA (RAPD) analysis in Indian mung bean [V. radiata (L.) Wilczek] cultivars. Genetica, 109: 227-234.

  14. Reddy, K.S., Souframanien, J., Reddy, K.S. and Dhanasekar, P. (2008). Genetic diversity in mung bean as revealed by SSR and ISSR markers. Journal of Food Legumes, 21: 15- 21.

  15. Rohlf, F.J. (2004). NTSYS-PC. Numerical Taxonomy and Multivariate Analysis System. Exeter Software, New York.

  16. Saini, M., Singh, S., Hussain, Z. and Yadav, A. (2010). RAPD analysis in mungbean (V. radiata (L.) Wilczek): I. Assessment of genetic diversity. Indian Journal of Biotechnology, 9: 137-146.

  17. Samarajeewa, P.K., Nanayakkara, H.L.V., Ekanayake, E.M.D.S.N. and Sumanasinghe, V.A. (2002). RAPD analysis of genetic relationships of wild and cultivated Vigna species. Annals of Sri Lanka Department of Agriculture, 4: 215-226.

  18. Sambrook, J., Fritschi, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, New York. 

  19. Santalla, M., Power, J.B. and Davey, M.R. (1998). Genetic diversity in mungbean germplasm revealed by RAPD markers. Plant Breeding, 117: 473-478.

  20. Shafique, S., Khan, M.R., Nisar, M. and Rehman, S.U. (2011). Investigation of genetic diversity in black gram [Vigna mungo (L.) Hepper]. Pakistan Journal of Botany, 43: 1223- 1232.

  21. Smith, J.S.C., Chin, E.C.L., Shu, H., Smith, O.S., Wall, S.J., Senior, M.L., Mitchell, S.E., Kresovich, S. and Zeigle, J. (1997). An evaluation of the utility of SSR loci as molecular markers in maize (Z. mays L.): comparisons with data from RFLPs and pedigree. Theoretical and Applied Genetics, 95: 163-173.

  22. Sneath, P.H.A. and Sokal, R.R. (1973). Numerical Taxonomy. San Francisco: Freeman.

  23. Sonnante, G. and Beckmann, J.S. (1997). Using RAPD markers to investigate genetic variation in chickpea. Journal of Genetic Breeding, 51: 303-307.

  24. Souframanien, J., Pawar, S.E. and Rucha, A.G. (2002). Genetic variation in gamma ray induced mutants in black gram as revealed by RAPD and ISSR markers. Indian Journal of Genetic, 4: 291-295.

  25. Srivastava, P., Pandey, A. and Sinha, D.P. (2013). Genetic diversity analysis in different varieties of black gram using RAPD markers. International Journal of Biological Research, 1: 43-48.

  26. Suneja, Y., Kaur, S., Gupta, A.K. and Kaur, N. (2012). Molecular evaluation of genetic Variability in black gram genotypes using RAPD Markers. Indian Journal Agricultural Biochemistry, 25: 36-43.

  27. Talebi, R., Naji, A.M. and Fayaz, F. (2008). Geographical patterns of genetic diversity in cultivated chickpea (C. arietinum L.) characterized by amplified fragment length polymorphism. Plant Soil Environment, 54: 447-452.

  28. Williams, G.K., Kubelik, A.R., Livak, K.L., Rafalshi, J.A. and Tingey, S.V. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Research, 18: 6531-6535.

  29. Yee, E., Kidwell, K.K., Sills, G.R. and Lumpkin, T.A. (1999). Diversity among selected Vigna angularis (Azuki) accessions on the basis of RAPD and AFLP markers. Crop Sciences, 39: 268-275.

     
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