Somaclonal variation for genetic improvement in grasspea (Lathyrus sativus L.)

DOI: 10.18805/lr.v0iOF.6853    | Article Id: LR-3326 | Page : 329-335
Citation :- Somaclonal variation for genetic improvement in grasspea (Lathyrus sativus L.) .Legume Research-An International Journal.2016.(39):329-335

Swapan K. Tripathy*, A. Panda, Pramod K. Nayak, Sasmita Dash, D. Lenka, D.R. Mishra, Rajesh K. Kar, N. Senapati and G.B. Dash

swapankumartripathy@gmail.com
Address :

Sinha Molecular Breeding Laboratory, Department of Plant Breeding & Genetics, College of Agriculture, OUAT, Bhubaneswar-751 003, India

Submitted Date : 21-01-2014
Accepted Date : 6-06-2014

Abstract

Somaclonal variation was revealed among regenerants in four genotypes (Nirmal, P24, Nayagarh local and Dhenkanal local) of grasspea (Lathyrus sativus L.) following long term in vitro culture of internode explants. Most of the somaclones which survived till maturity had revealed chromosomal abnormalities and variation in one or more morphological and/or agronomic traits. Some of the variant phenotypes especially those relating to developmental variations were not inherited to R2 generation owing to elimination of chromosomal aberrations that preclude normal morphogenesis. Somaclones with variation in flower colour, seed colour, leaflet length and breadth, foliage and pod pigmentation may be used as genetic markers in breeding Lathyrus sativus. Besides, variants with broad leaf, dwarf height, long pod, large seed, short duration and synchronous maturity are agronomically desirable. A large seeded somaclone NGOG 5 having high seed yield and low neurotoxin content (ODAP) recovered in this pursuit, can be a desirable candidate for future breeding programme.

Keywords

Lathyrus sativus L Morphological and agronomic traits Somaclonal variation Tissue culture instability.

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