nifH gene diversity and symbiotic effectiveness of Bradyrhizobium yuanmingense strains obtained from nodules of soybean cultivated in India

DOI: 10.5958/0976-0571.2015.00085.5    | Article Id: LR-3248 | Page : 271-275
Citation :- nifH gene diversity and symbiotic effectiveness of Bradyrhizobium yuanmingense strains obtained from nodules of soybean cultivated in India.Legume Research.2015.(38):271-275
C. Appunu*, Ravinder Kumar, C. Mahadevaiah, V. Sreenivasa and T. Manjunatha cappunu@gmail.com
Address : Division of Plant Molecular Biology, M.S. Swaminathan Research Foundation, III Cross Street, Taramani Institutional Area, Chennai 600113, Tamil Nadu, India.


Restriction fragment length polymorphism (RFLP) of nifH gene with three endonucleases revealed three different nifH genotypes among twenty one indigenous Bradyrhizobium yuanmingense strains. The interactive symbiotic effectiveness on soybean cv. JS335, nodulation and plant growth of these B. yuanmingense strains were comparatively evaluated with B. japonicum ASR011 under greenhouse conditions. Significant variation in symbiotic potential among native B. yuanmingense strains was observed and few of them exhibited significantly superior symbiotic performance over B. japonicum ASR011 and B. yuanmingense CCBAU 61071 type strain. A considerable difference in nitrogen-fixing characteristics was detected among B. yuanmingense strains which shared identical nifH gene sequence. This, to our knowledge, is the first study on symbiotic effectiveness of B. yuanmingense strains obtained from nodules of soybean cultivated in India. The symbiotically efficient B. yuanmingense strains may have applications in the formulation of appropriate inocula to improve soybean crop yield in Indian soils.


B. yuanmingense Glycine max nifH gene diversity Nodulation.


  1. AOAC. (1990). Official Method of Analysis of the Association of Official Analytical Chemist, 15 Ed, Arlington, VA, 552.
  2. Appunu, C. and Dhar, B. (2006). Differential symbiotic response of phage-typed strains of Bradyrhizobium japonicum with soybean cultivars. J. Microbiol. 44(3): 363-368.
  3. Appunu, C., Ganesan, G., Kalita, M., Kaushik, R., Saranya, B., Prabavathy, V. R. and Nair, S. (2011). Phylogenetic diversity of rhizobia associated with Horsegram [Macrotyloma uniflorum (Lam.) Verdc.] grown in South India based on glnII, recA and 16S-23S intergenic sequence analyses. Curr. Microbiol. 62: 1230-1238.
  4. Appunu, C., N’Zoue, A. and Laguerre, G. (2008a). Genetic diversity of native bradyrhizobia isolated from soybeans (Glycine max L.) in different agricultural-ecological-climatic regions of India. Appl. Environ. Microbiol. 74: 5991-5996.
  5. Appunu, C., Sasi Rekha, N., Prabavathy, V. R. and Nair, S. (2009). A significant proportion of indigenous rhizobia from India associated with soybean [Glycine max (L.)] are distinctly belong to Bradyrhizobium and Ensifer genera. Biol. Fertil. Soils 46: 57-63.
  6. Appunu, C., Sen, D., Singh, M. K. and Dhar, B. (2008b). Variation in symbiotic performance of Bradyrhizobium japonicum strains and soybean cultivars under field conditions. J. Central European Agri. 9: 185-190.
  7. Hungria, M., Campo, R.J. and Mendes, I.C. (2003). Benefits of inoculation on common bean (Phaseolus vulgaris) crop with efficient and competitive Rhizobium tropicii strains. Biol. Fertil. Soils 39: 88-93.
  8. Hungria, M., Campo, R.J., Chueire, L.M.O., Grange, L. and Megias, M. (2001). Symbiotic effectiness of fast-growing rhizobial strains isolated from soybean nodules in Brazil. Biol. Fertil. Soils 33: 387-394.
  9. Meghvanshi, M.K., Prasad, K. and Mahna, S.K. (2010). Symbiotic potential, competitiveness and compatibility of indigenous Bradyrhizobium japonicum isolates to three soybean genotypes of two distinct agro-climatic regions of Rajasthan, India. Saudi J. Biol. Sci. 17: 303-310.
  10. Melchiorre, M., de Luca, M.J., Anta, G.G., Suarez, P., Lopez, C., Lascano, R. and Racca, R.W. (2011). Evaluation of bradyrhizobia strains isolated from field-grown soybean plants in Argentina as improved inoculants. Biol. Fertil. Soils 47: 81-89.
  11. Pule-Meulenberg, F., Gyogluu, C., Naab, J. and Dakora, F.D. (2011). Symbiotic N nutrition, bradyrhizobial diversity and photosynthetic functioning of six inoculated promiscuous-nodulating soybean genotypes. J. Plant Physiol. 168: 540-548.
  12. Risal, C.P., Yokoyama, T., Ohkama-Ohtsu, N., Djedidi, S. and Sekimoto, H. (2010). Genetic diversity of native soybean bradyrhizobia from different topographical regions along the southern slopes of the Himalayan mountains in Nepal. Syst. Appl. Microbiol. 33: 416-425.
  13. Sarr, P.S., Yamakawa, T., Fujimoto, S., Saeki, Y., Thao, H.T.B. and Myint, A.K. (2009). Phylogenetic diversity and symbiotic effectiveness of root-nodulating bacteria associated with cowpea in the South-West area of Japan. Microbes Environ. 24: 105-112.
  14. Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S. (2011). MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol. Biol. Evol. 28: 2731-2739.
  15. Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. and Higgins, D.G. (1997). The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nuc. Acids Res. 25: 4876-4882.
  16. Vincent, J. (1970). A manual for the practical study of the root nodule bacteria. In: Int. Biol. Prog. Handbook, vol. 15. Blackwell Scientific Publications (Oxford, USA).
  17. Vinuesa, P., Rojas-Jimenez, K., Contreras-Moreira, B., Mahna, S.K., Prasad, B.N., Moe, H., Selvaraju, S.B., Thierfelder, H. and Werner, D. (2008). Multilocus sequence analysis for assessment of the biogeography and evolutionary genetics of four Bradyrhizobium species that nodulate soybeans on the Asiatic continent. Appl. Environ. Microbiol. 74: 6987-6996.
  18. Yang, J.K. and Zhou, J.C. (2008). Diversity, phylogeny and host specificity of soybean and peanut bradyrhizobia. Biol. Fertil. Soils 44: 843-851.
  19. Yao, Z.Y., Kan, F.L., Wang, E.T, Wei, G.H. and Chen, W.X. (2002). Characterization of rhizobia that nodulate legume species of the genus Lespedeza and description of Bradyrhizobium yuanmingense sp. Nov. Int. J. Syst. Evol. Microbiol. 52: 2219-2230.

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