Legume Research

  • Chief EditorJ. S. Sandhu

  • Print ISSN 0250-5371

  • Online ISSN 0976-0571

  • NAAS Rating 6.80

  • SJR 0.391

  • Impact Factor 0.8 (2023)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus

Proteome alteration of soybean as a function of pod distortion syndrome

Kamal Payghamzadeh, Mahmood Toorchi*, Zahra Sadat Shobbar

Pod distortion syndrome (PDS) is a particular type of growth in which soybean plants remain green long after pod maturation. The aim of this study was to assess protein profiles of PDS and non-PDS soybeans via proteomics approaches. Therefore, protein expression profiles of PDS and non-PDS soybean cultivars viz. Katul and Gorgan 3 were analyzed by nESI-LC-MS/MS. Comparative analysis of significant proteins via nESI-LC-MS/MS revealed that 5 and 11 proteins in Gorgan 3 had significantly different expression levels in PDS and non-PDS, respectively. Most  of these proteins had already been known to regulate diverse cellular activities e.g. energy production, metabolism, signal transduction, gene transcription and translation as well as protein destination and storage. But, the present findings suggest that the key regulators of PDS in soybean plants may be are 14-3-3 like protein, Nascent Polypeptide-Associated Complex Alpha Subunit, Rubisco large subunit, and oxygen evolving enhancer protein 2 protein.

  1. Ahmadi, M.R. and Zelentsov, S. V (2002). Reason study of forming the anomalously developed soybean pods on Iran north. Electron. J. “Investigated Russ.: 609–615.

  2. Arnon, A.N. (1967). Method of extraction of chlorophyll in the plants. Agron. J. 23: 112–121.

  3. Bahrman, N., Le Gouis, J., Negroni, L., Amilhat, L., Leroy, P., Lainé, A.A.-L. and Jaminon, O. (2004). Differential protein expression assessed by two dimensional gel electrophoresis for two wheat varieties grown at four nitrogen levels. Proteomics 4: 709–719.

  4. Bevan, M., I. Bancroft, E. Bent, K. Love, H. Goodman, C. Dean, R. Bergkamp, W. Dirkse, M. Van Staveren, et al and Chalwatzis, N. (1998). Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana. Nature 391: 485–488.

  5. Bradford, M.M. (1976). Rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein–dye biding. Anal. Biochem. 72: 248.

  6. Breiman, A., Fieulaine, S., Meinnel, T. and Giglione, C. (2016). The intriguing realm of protein biogenesis: Facing the green co-translational protein maturation networks. Biochim. Biophys. Acta - Proteins Proteomics 1864: 531–550.

  7. Cotelle, V. and Leonhardt, N. (2016). 14-3-3 Proteins in Guard Cell Signaling. Front. Plant Sci. 6(January): 1–10.

  8. Cottrell, J.S. (2011). Protein identification using MS/MS data. J. Proteomics 74: 1842–51.

  9. Damerval, C., De Vienne, D., Zivy, M. and Thiellement, H. (1986). Technical improvements in two dimensional electrophoresis increase the level of genetic variation detected in wheat seedling proteins. Electrophoresis 7: 52–54.

  10. Ellis, R.J. (2006). Molecular chaperones: assisting assembly in addition to folding. Trends Biochem. Sci. 31: 395–401.

  11. Ellis, R.J. and Van Der Vies, S.M. (1988). The Rubisco subunit binding protein. Photosynth. Res. 16: 101–115.

  12. Fait, A., Fromm, H., Walter, D., Galili, G. and Fernie, A.R. (2008). Highway or byway: the metabolic role of the GABA shunt in plants. Trends Plant Sci. 13: 14–19.

  13. Fu, H., Subramanian, R.R. and Masters, S.C. (2000). 14-3-3 proteins: structure, function, and regulation. Annu. Rev. Pharmacol. Toxicol. 40: 617–647.

  14. He, Z., von Caemmerer, S., Hudson, G.S., Price, G.D., Badger, M.R. and Andrews, T.J. (1997). Ribulose-1, 5-bisphosphate carboxylase/oxygenase activase deficiency delays senescence of ribulose-1, 5-bisphosphate carboxylase/oxygenase but progressively impairs its catalysis during tobacco leaf development. Plant Physiol. 115: 1569–1580.

  15. Hill, C.B., Hartman, G.L., Esgar, R. and Hobbs, H. A. (2006). Field evaluation of green stem disorder in soybean cultivars. Crop Sci. 46: 879–885.

  16. Hossain, Z., Khatoon, A. and Komatsu, S. (2013). Soybean proteomics for unraveling abiotic stress response mechanism. J. Proteome Res. 12: 4670–4684.

  17. Lee, S.-E.E., Yoo, S.Y., Kim, D.-Y.Y., Ko, T.S., Ok, Y.S. and Kim, T.-W.W. (2014). Proteomic evaluation of the response of soybean (Glycine max var Seoritae) leaves to UV-B. Plant Omics 7: 123–132.

  18. McCormack, a L., Schieltz, D.M., Goode, B., Yang, S., Barnes, G., Drubin, D. and Yates, J.R. (1997). Direct analysis and identification of proteins in mixtures by LC/MS/MS and database searching at the low-femtomole level. Anal. Chem. 69: 767–776.

  19. Merewitz, E.B., Gianfagna, T. and Huang, B. (2011). Protein accumulation in leaves and roots associated with improved drought tolerance in creeping bentgrass expressing an ipt gene for cytokinin synthesis. J. Exp. Bot. 62: 5311–5333.

  20. Miernyk, J.A. (1999). Protein folding in the plant cell. Plant Physiol. 121: 695–703.

  21. Murithi, H.M., Beed, F., Tukamuhabwa, P., Thomma, B.P.H.J. and Joosten, M.H.A.J. (2016). Soybean production in eastern and southern Africa and threat of yield loss due to soybean rust caused by Phakopsora pachyrhizi. Plant Pathol. 65: 176–188.

  22. Nanjo, Y., Maruyama, K., Yasue, H., Yamaguchi-Shinozaki, K., Shinozaki, K. and Komatsu, S. (2011). Transcriptional responses to flooding stress in roots including hypocotyl of soybean seedlings. Plant Mol. Biol. 77: 129–144.

  23. Nouri, M.-Z. and Komatsu, S. (2010). Comparative analysis of soybean plasma membrane proteins under osmotic stress using gel based and LC MS/MS based proteomics approaches. Proteomics 10: 1930–1945.

  24. O’Farrell, P.H. (1975). High Resolution Two-Dimensional Electrophoresis of Proteins. J Biol Chem 250: 4007–4021.

  25. Pink, M., Verma, N., Rettenmeier, A.W. and Schmitz-Spanke, S. (2010). CBB staining protocol with higher sensitivity and mass spectrometric compatibility. Electrophoresis 31: 593–598.

  26. Rokka, A., Zhang, L. and Aro, E.M. (2001). Rubisco activase: an enzyme with a temperature dependent dual function? Plant J. 25: 463–471.

  27. Rospert, S., Dubaquie, Y., Gautschi, M., Dubaquié, Y. and Gautschi, M. (2002). Nascent-polypeptide-associated complex. Cell. Mol. Life Sci. 59: 1632–1639.

  28. SAS Institute. (1997). SAS/STAT. Usersguid. [Version 6.12]. Cary, NC, SAS Inst.

  29. Shimada, S., OYA, T., Nakamura, T., Hattori, M., Nakayama, N., Shimamura, S., Yamamoto, R. and Kim, Y.-H. (2005). The Occurrence of Green Stem Syndrome with Different varieties, Planting Date and Depodding Treatment in Soybeans. p. 8518. In The ASA-CSSA-SSSA International Annual Meetings, Salt Lake City, UT.

  30. Sözen, E. (2004). Vegetative Storage Proteins in Plants. Anadolu Univ. J. Sci. Technol. 5: 1–7.

  31. Thomas, H. (2013). Senescence, ageing and death of the whole plant. New Phytol. 197: 696–711.

  32. Wang, L., Ma, H., Song, L., Shu, Y. and Gu, W. (2012). Comparative proteomics analysis reveals the mechanism of pre-    harvest seed deterioration of soybean under high temperature and humidity stress. J. Proteomics 75: 2109–2127.

  33. Wittenbach, V.A. (1983). Purification and Characterization of a Soybean Leaf Storage Glycoprotein. Plant Physiol. 73: 125–129.

  34. Xu, C. and Huang, B. (2010). Differential proteomic response to heat stress in thermal Agrostis scabra and heat sensitive Agrostis stolonifera. Physiol. Plant. 139: 192–204.

  35. Yin, Y., Yang, R. and Gu, Z. (2014). Organ-specific proteomic analysis of NaCl-stressed germinating soybeans. J. Agric. Food Chem. 62: 7233–7244.

  36. Zörb, C., Schmitt, S. and Mühling, K.H. (2010). Proteomic changes in maize roots after short term adjustment to saline growth conditions. Proteomics. 10: 4441–4449. 

Editorial Board

View all (0)