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Current IssueEditorial BoardOnline First ArticlesArchive

volume 38 issue 4 (august 2015) : 447-451,   Doi: 10.5958/0976-0571.2015.00039.9

Breeding mungbean [Vigna radiata (L.) Wilczek] for development of high yielding protein rich lines with low trypsin inhibitor

S
S. Das
D
Dewanjee
A
A. Maji
K
K.K. Sarkar
1Department of Plant Breeding, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur- 741 252, Nadia, India.
Cite article:- Das S., Dewanjee, Maji A., Sarkar K.K. (2025). Breeding mungbean [Vigna radiata (L.) Wilczek] for development of high yielding protein rich lines with low trypsin inhibitor . Legume Research. 38(4): 447-451. doi: 10.5958/0976-0571.2015.00039.9.

ABSTRACT

Genetic diversity among twelve mungbean genotypes was estimated following SDS-PAGE analysis. The genotypes were also evaluated for their per-se performance on yield, protein content alongwith presence of trypsin inhibitor in seed. The trypsin inhibitor ranged from 1324.26 TIU/g in PDM 54 to 1502 TIU/g in IC 311420. The protein content ranged from 14.31% in IC 325817 to 36.56% PDM 84 143 and yield plant-1 was recorded as low as 2.64g in IC 324036 to as high as 12.46 in IC 333153. PDM 54 was also found to be good yielder. IC 333153, PDM 54 and Hinjalgunj with low trypsin content were crossed with PDM 84143 to develop high yielding protein rich lines with low level of trypsin inhibitor. The hybrid between IC 333153 and PDM 84143 showed high heterosis towards desired direction for all of these three traits.

REFERENCES

  1. Asghar R., Siddique T. and Afzal M. (2003) Inter and intra-specific variation in SDS-PAGE electrophorograms of total seed protein in chickpea(Cicer arietinum L.) Germplasm. Pak. J. Bio. Sci. 6(24): 1991-1995
  2. Chattopadhya K., Sarkar H.K., and Bhattacharyya S. (2009) Morphological and nutritional Diversity of some mungbean genotypes from Indian subcontinent. Indian J. Plant Genet Resour., 22(2): 155-159
  3. Dasgupta T., and Singh M. (2003) Diversity in advanced breeding lines of chickpea. Int. Chickpea Pigeon pea Newslett., 10: 38-41.
  4. Ferreira J.J., lvarrez E.A., Roca M.A. and Giraldez R. (2000) Determination of the out crossing rate of Phaseolus vulgaris L. using seed protein markers. Euphytica, 113: 259-263
  5. Fillippetti A. and Azadegan G.H. (1994) Protein content and trypsin inhibitor in raw fababean seeds. FABIS Newslette., 34- 35: 48-51
  6. Ghafoor A., Gulbazz F.N, Afzal M., Ashraf M. and Arshad M. (2003) Inter-relationship between SDSPAGE markers and agronomic characters in chickpea(Cicer arietinum L.). Pak. J. Bot. 35: 613-624
  7. Ghafoor A. and Ahmad Z. (2005) Diversity of agronomic traits and total seed protein in blackgram Vigna mungo (L.) Hepper. Acta Biologia Cracoviensia Series Botanica., 47(2): 69-75
  8. Hong J.W. Na L. and Hong D.L.(2006) Genetic diversity of seed storage proteins in different ecotype varieties of japonica rice and its application. Rice Sci., 13: 85-92
  9. Kakade M L, Rackis J J, McGhee J E and Puski G (1974) Determination of trypsin inhibitor activity of Soy products : a collaborative analysis of an improved procedure. Cereal Chem., 51: 376-382
  10. Lowry O.H., Rosenbought N.J., Ferr A.L. and Randall R.J. (1951) Protein measurement with the folin phenol reagent. Journal of Biological Chemistry. 193: 265-275
  11. Rahman M.M. and Hirata Y., (2004) Genetic diversity in Brassica species using SDS-PAGE analysis. J. Biol. Sci. 4: 234-238.
  12. Ramesh Babu S. and Subrah manyam B. ( 2011) Evaluation of trypsin and chymotrypsin proteinase inhibitors in germplasm of Indian food legumes. J. of Food Legumes. 24(4): 338-340
  13. Sneath P.H.A. and Sokal R.R. (1973) Numerical Taxonomy. W.H. Truman & Co., San Francisco
  14. Thuzar Win, Zaw Oo K. , Lay New A., San Thein K.M and Yutaka H. (2011) Diversity of Myanmar cowpea accessions through seed storage polypeptides and its cross compatibility with the subgenus Ceratotropis. J. of Pl. Breed. And Crop Sci. Vol. 3(5), pp. 87-95
  15. Weber H., Borisjuk L. and Wobus U. (2005) Molecular physiology of legume seed development. Ann. Rev. Plant Biol. 56: 253-279.
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    volume 38 issue 4 (august 2015) : 447-451,   Doi: 10.5958/0976-0571.2015.00039.9

    Breeding mungbean [Vigna radiata (L.) Wilczek] for development of high yielding protein rich lines with low trypsin inhibitor

    S
    S. Das
    D
    Dewanjee
    A
    A. Maji
    K
    K.K. Sarkar
    1Department of Plant Breeding, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur- 741 252, Nadia, India.
    Cite article:- Das S., Dewanjee, Maji A., Sarkar K.K. (2025). Breeding mungbean [Vigna radiata (L.) Wilczek] for development of high yielding protein rich lines with low trypsin inhibitor . Legume Research. 38(4): 447-451. doi: 10.5958/0976-0571.2015.00039.9.

    ABSTRACT

    Genetic diversity among twelve mungbean genotypes was estimated following SDS-PAGE analysis. The genotypes were also evaluated for their per-se performance on yield, protein content alongwith presence of trypsin inhibitor in seed. The trypsin inhibitor ranged from 1324.26 TIU/g in PDM 54 to 1502 TIU/g in IC 311420. The protein content ranged from 14.31% in IC 325817 to 36.56% PDM 84 143 and yield plant-1 was recorded as low as 2.64g in IC 324036 to as high as 12.46 in IC 333153. PDM 54 was also found to be good yielder. IC 333153, PDM 54 and Hinjalgunj with low trypsin content were crossed with PDM 84143 to develop high yielding protein rich lines with low level of trypsin inhibitor. The hybrid between IC 333153 and PDM 84143 showed high heterosis towards desired direction for all of these three traits.

    REFERENCES

    1. Asghar R., Siddique T. and Afzal M. (2003) Inter and intra-specific variation in SDS-PAGE electrophorograms of total seed protein in chickpea(Cicer arietinum L.) Germplasm. Pak. J. Bio. Sci. 6(24): 1991-1995
    2. Chattopadhya K., Sarkar H.K., and Bhattacharyya S. (2009) Morphological and nutritional Diversity of some mungbean genotypes from Indian subcontinent. Indian J. Plant Genet Resour., 22(2): 155-159
    3. Dasgupta T., and Singh M. (2003) Diversity in advanced breeding lines of chickpea. Int. Chickpea Pigeon pea Newslett., 10: 38-41.
    4. Ferreira J.J., lvarrez E.A., Roca M.A. and Giraldez R. (2000) Determination of the out crossing rate of Phaseolus vulgaris L. using seed protein markers. Euphytica, 113: 259-263
    5. Fillippetti A. and Azadegan G.H. (1994) Protein content and trypsin inhibitor in raw fababean seeds. FABIS Newslette., 34- 35: 48-51
    6. Ghafoor A., Gulbazz F.N, Afzal M., Ashraf M. and Arshad M. (2003) Inter-relationship between SDSPAGE markers and agronomic characters in chickpea(Cicer arietinum L.). Pak. J. Bot. 35: 613-624
    7. Ghafoor A. and Ahmad Z. (2005) Diversity of agronomic traits and total seed protein in blackgram Vigna mungo (L.) Hepper. Acta Biologia Cracoviensia Series Botanica., 47(2): 69-75
    8. Hong J.W. Na L. and Hong D.L.(2006) Genetic diversity of seed storage proteins in different ecotype varieties of japonica rice and its application. Rice Sci., 13: 85-92
    9. Kakade M L, Rackis J J, McGhee J E and Puski G (1974) Determination of trypsin inhibitor activity of Soy products : a collaborative analysis of an improved procedure. Cereal Chem., 51: 376-382
    10. Lowry O.H., Rosenbought N.J., Ferr A.L. and Randall R.J. (1951) Protein measurement with the folin phenol reagent. Journal of Biological Chemistry. 193: 265-275
    11. Rahman M.M. and Hirata Y., (2004) Genetic diversity in Brassica species using SDS-PAGE analysis. J. Biol. Sci. 4: 234-238.
    12. Ramesh Babu S. and Subrah manyam B. ( 2011) Evaluation of trypsin and chymotrypsin proteinase inhibitors in germplasm of Indian food legumes. J. of Food Legumes. 24(4): 338-340
    13. Sneath P.H.A. and Sokal R.R. (1973) Numerical Taxonomy. W.H. Truman & Co., San Francisco
    14. Thuzar Win, Zaw Oo K. , Lay New A., San Thein K.M and Yutaka H. (2011) Diversity of Myanmar cowpea accessions through seed storage polypeptides and its cross compatibility with the subgenus Ceratotropis. J. of Pl. Breed. And Crop Sci. Vol. 3(5), pp. 87-95
    15. Weber H., Borisjuk L. and Wobus U. (2005) Molecular physiology of legume seed development. Ann. Rev. Plant Biol. 56: 253-279.
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