Legume Research

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Research Article

Legume Research, volume 40 issue 5 (october 2017) : 830-835

Caucasian clover (Trifolium ambiguum Bieb.) × white clover (T. repens L.) – interspecific hybrids developed through tissue culture

Fan Huang, Mingjiu Wang, Zhiyong Li
1Grassland Research Institute, Chinese Academy of Agricultural Sciences, Hohhot-010 010, China.

  • Submitted13-09-2016|

  • Accepted17-02-2017|

  • First Online 18-09-2017|

  • doi 10.18805/LR-317

Cite article:- Huang Fan, Wang Mingjiu, Li Zhiyong (2017). Caucasian clover (Trifolium ambiguum Bieb.) × white clover (T. repens L.) – interspecific hybrids developed through tissue culture. Legume Research. 40(5): 830-835. doi: 10.18805/LR-317.

ABSTRACT

In order to create new forage variety, caucasian clover (Trifolium ambiguum Bieb.) with strong tolerance to drought and cold introduced from New Zealand as the female parent was hybridized with a white clover (T. repens L.) collected from northeast China, which has strong nitrogen fixation capacity. Hybrid embryos of caucasian clover×white clover were successfully raised using embryo rescue. 13.8% of these embryos grew to maturity. The successfully grown plants were transferred to sterilized vermiculite after 5-6 weeks and then to field after 3-4 months. The hybrid plant showed intermediate morphological features and a chromosomal number of 2n=5X=40. In order to obtain more plants for backcross, a tissue culture system was established for the hybrids, using MS+0.1mg l-1 2, 4-D+2mg l-1 6-BA, MS +0.5mg l-1 NAA+1mg l-1 6-BA+1mg l-1 KT and 1/2MS as the medium for bud-induction, differentiation and root-induction respectively. 80% of the cultured plantlets survived these growing conditions. Hybridity of the plant was also confirmed by ISSR markers using 16 primers.

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