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Current IssueEditorial BoardOnline First ArticlesArchive

volume 38 issue 1 (february 2015) : 115-120,   Doi: 10.5958/0976-0571.2015.00019.3

Antagonistic effect of Mesorhizobium ciceri Isolates on Fusarium oxysporum F. Sp. Ciceri

S
Suman*
A
A.S. Yadav
1Department of Genetics & Plant Breeding, CCS Haryana Agricultural University, Hisar-125004, India.
Cite article:- Suman*, Yadav A.S. (2025). Antagonistic effect of Mesorhizobium ciceri Isolates on Fusarium oxysporum F. Sp. Ciceri. Legume Research. 38(1): 115-120. doi: 10.5958/0976-0571.2015.00019.3.

ABSTRACT

A total of 207 wild type (WT)  isolates of Mesorhizobium ciceri,  isolated from root nodules of chickpea plants, was screened for antagonistic effect to Fusarium oxysporum f.sp. ciceri on TY medium by spot test method. Seven isolates (MC69, MC84, MC96, MC99, MC180, MC183 and MC190) were Nod+ and found to be antagonistic to  Fusarium oxysporum f.sp. ciceri by showing inhibition zone, but none produced antibiotic or solubilize tricalcium phosphate. However, these produced indole acetic acid (IAA), which ranged from 200.2 mg/ml to 1762.7 mg/ml. Three isolates (MC84, MC96 and MC99) produced siderophore by making orange halo zone on blue coloured plates. The MC99 was found to be best antagonistic isolate as it produced the maximum amount of siderophore and IAA.

REFERENCES

  1. Alikhani, H.A., Rastin, N.S. and Antoun, H. (2006). Phosphate solubilization activity of rhizobia native to Iranian soils. Pl. Soil., 287: 35-41.
  2. Berg, G., Roskot, N., Steidle, A., Eberl, L., Zock, A. and Smalla, K. (2002). Plant dependent genotypic and phynotypic diversity of antagonistic rhizobacteria isolated from different Verticillium host plants. Appl. Environ. Microbiol., 68: 3328-3338.
  3. Beringer, J.E. (1974). R factor transfer in Rhizobium leguminosarum. J. Gen. Microbiol., 84: 188-198.
  4. Biswas, J.C., Lodha, J.K. and Dazzo, F.B. (2000). Rhizobia inoculation improves nutrient uptake and growth of lowland rice. Soil Sci. Soc. Am. J., 64: 1644-1650.
  5. Castillo, U.F., Strobel, G.A., Ford, E.J., Hess, W.N., Porter, H., Jensen, J.B., Albert., H.,Condron, M.A.M., Teplow, D.B., Steevens, D. and Yaver, D. (2002). Munumbicins , wide spectrum antibiotics produced by Streptomyces NRRL 30562, endophytic on Kennedia nigricans Microbiol. 148:2675-2685.
  6. Chakraborty, U. and Purkayastha, R.P. (1984). Role of rhizobitoxine in protecting soybean roots. Can. J. Microbiol., 30: 285-289.
  7. Coombs, J.T., Michelson, P.P. and Franco, C.M.M. (2004). Evaluation of endophytic actinobacteria as antagonists of Gaeumanomyces gramini var. tritici in wheat. Biol. Control: Theory and application in pest management., 29: 359-366.
  8. Deshwal, V.K., Dubey, R.C. and Maheshwari, D.K. (2003b). Isolation of plant growth promoting strains of Bradyrhizobium (Arachis) sp. with biocontrol potential against Macrophomina phaseolina causing charcoal rot of peanut. Curr. Sci., 84: 443-448.
  9. Enteshamul-Haque, S. and Ghaffar, A. (1993). Use of rhizobia in the control of root rot disease of sunflower, okra, soybean and mungbean. J. Phytopathol., 138: 157-163.
  10. Ghosh, A. C. and Basu, P.S. (2002). Growth behaviour and biproduction of indole acetic acid by Rhizobium species isolated from root nodules of a leguminous tree Dalbergia lanceolaria. Ind. J. Exp. Biol., 40: 796-801.
  11. Glickman, E. and Dessaux, Y. (1995). A critical examination of the specificity of the Salkowski reagent for indole compounds produced by pathogenic bacteria. Appl. Environ. Microbiol., 61: 793-796.
  12. Handelsman, J. and Stabb, E.V. (1996). Biocontrol of soil borne plant pathogens. The Plant Cell: 8: 1855-1869.
  13. Hartman, A., Singh, M. and Klingmuler, W. (1983). Isolation and characterization of Azospirillum mutants excreting high amount of indole acetic acid. Can. J. Microbiol., 29: 916-923.
  14. Howell, C.R. and Stipanovic, R.D. Suppression of Pythium ultimum induced damping off of cotton seedlings by Pseudomonas fluorescence and its antibiotic, pyoluteorin. (1980). Phytopathol. 70:712-715.
  15. Idris, H.A.,Labuschagne, N. and Korsten, L. (2007). Screening of rhizobacteria for biocontrol of Fusarium root and crown rot of Sorghum in Ethiopia. Biol. Control. 40:97-106.
  16. Jaymani, A.K. (2006). Studies on antagonistic effect of rhizobacteria against soil borne Phytophthora species in strawberry. M.Sc. Thesis. Der Universtat Hanover.
  17. Lemessa, F. and Zeller, W. (2007). Screening of rhizobacteria for biological control of Ralstonia solanacearum in Ethiopia. Biol. Control., 42: 336-344.
  18. Neilands, J.B. and Leong, S.A. (1986). Siderophores in relation to plant growth and disease. Ann. Rev. Pl. Physiol., 37:187-208.
  19. Park, M., Paul, D., Kim, Y.K., Nam, K.W., Lee, Y.K., Choi, H.W. and Lee, S.Y. (2007). Induced systemic resistance by Bacillus vallismortis EXTN-1 suppressed bacterial wilt in tomato caused by Ralstonia solanacearum. Pl. Pathol., 23: 22-25.
  20. Persmark, M., Pittman, P., Buyer, J.S., Schwyn, B., Gill, P.R. and Neiland, J.B. Isolation and structure of Rhizobactin 1021, a siderophore from the alfalfa symbiont Rhizobium meliloti 1021. (1993). J. Am. Chem. Soc., 115: 3950-3956.
  21. Pikovskaya, R.I. (1948). Mobilization of phosphorus in soil in connection with the vital activity of some microbial species. Microbiologica., 17: 362-370.
  22. Romero, D., de Viucente, A., Rakotoaly, R.H., Dufour, S.E., Veening, J.W., Arrebola, E., Cazorla, F.N., Quipers, O.P., Paquot, M. and Perez-Garcia, A. (2007). The Iturin and Fengycin families of lipopeptides are key factors in antagonisms of Bacillus subtilis towards Podoshaera fusca. Mol. Pl. Microbe Interact., 20: 430-440.
  23. Schwyn, B. and Neilands, J.B. Universal chemical assay for detection and determination of siderophore. (1987). Anal. Biochem., 160: 47-56.
  24. Sessitsch, A., Reiter, B. and Berg, G. (2004). Endophytic bacterial communities of field grown potato plants and their plant growth promoting and antagonistic abilities. Can. J. Microbiol., 50: 239-249.
  25. Sindhu, S.S., Gupta, S.K. and Dadarwal, K.R. (1999). Antagonistic effect of Pseudomonas spp. on pathogenic fungi and enhanced plant growth in green gram (Vigna radiata). Biol. Fertil. Soil., 29: 62-68.
  26. Sridevi, N. and Mallaiah, K.V. (2008). Production of indole-3-acetic acid by Rhizobium isolates from Sesbania species. Pl. Sci. Res., 1: 13-16.
  27. Vincent, J.M. (1970). A Manual for the Practical Study of Root Nodule Bacteria. Blackwell Scientific Publications, Oxford, England.
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    volume 38 issue 1 (february 2015) : 115-120,   Doi: 10.5958/0976-0571.2015.00019.3

    Antagonistic effect of Mesorhizobium ciceri Isolates on Fusarium oxysporum F. Sp. Ciceri

    S
    Suman*
    A
    A.S. Yadav
    1Department of Genetics & Plant Breeding, CCS Haryana Agricultural University, Hisar-125004, India.
    Cite article:- Suman*, Yadav A.S. (2025). Antagonistic effect of Mesorhizobium ciceri Isolates on Fusarium oxysporum F. Sp. Ciceri. Legume Research. 38(1): 115-120. doi: 10.5958/0976-0571.2015.00019.3.

    ABSTRACT

    A total of 207 wild type (WT)  isolates of Mesorhizobium ciceri,  isolated from root nodules of chickpea plants, was screened for antagonistic effect to Fusarium oxysporum f.sp. ciceri on TY medium by spot test method. Seven isolates (MC69, MC84, MC96, MC99, MC180, MC183 and MC190) were Nod+ and found to be antagonistic to  Fusarium oxysporum f.sp. ciceri by showing inhibition zone, but none produced antibiotic or solubilize tricalcium phosphate. However, these produced indole acetic acid (IAA), which ranged from 200.2 mg/ml to 1762.7 mg/ml. Three isolates (MC84, MC96 and MC99) produced siderophore by making orange halo zone on blue coloured plates. The MC99 was found to be best antagonistic isolate as it produced the maximum amount of siderophore and IAA.

    REFERENCES

    1. Alikhani, H.A., Rastin, N.S. and Antoun, H. (2006). Phosphate solubilization activity of rhizobia native to Iranian soils. Pl. Soil., 287: 35-41.
    2. Berg, G., Roskot, N., Steidle, A., Eberl, L., Zock, A. and Smalla, K. (2002). Plant dependent genotypic and phynotypic diversity of antagonistic rhizobacteria isolated from different Verticillium host plants. Appl. Environ. Microbiol., 68: 3328-3338.
    3. Beringer, J.E. (1974). R factor transfer in Rhizobium leguminosarum. J. Gen. Microbiol., 84: 188-198.
    4. Biswas, J.C., Lodha, J.K. and Dazzo, F.B. (2000). Rhizobia inoculation improves nutrient uptake and growth of lowland rice. Soil Sci. Soc. Am. J., 64: 1644-1650.
    5. Castillo, U.F., Strobel, G.A., Ford, E.J., Hess, W.N., Porter, H., Jensen, J.B., Albert., H.,Condron, M.A.M., Teplow, D.B., Steevens, D. and Yaver, D. (2002). Munumbicins , wide spectrum antibiotics produced by Streptomyces NRRL 30562, endophytic on Kennedia nigricans Microbiol. 148:2675-2685.
    6. Chakraborty, U. and Purkayastha, R.P. (1984). Role of rhizobitoxine in protecting soybean roots. Can. J. Microbiol., 30: 285-289.
    7. Coombs, J.T., Michelson, P.P. and Franco, C.M.M. (2004). Evaluation of endophytic actinobacteria as antagonists of Gaeumanomyces gramini var. tritici in wheat. Biol. Control: Theory and application in pest management., 29: 359-366.
    8. Deshwal, V.K., Dubey, R.C. and Maheshwari, D.K. (2003b). Isolation of plant growth promoting strains of Bradyrhizobium (Arachis) sp. with biocontrol potential against Macrophomina phaseolina causing charcoal rot of peanut. Curr. Sci., 84: 443-448.
    9. Enteshamul-Haque, S. and Ghaffar, A. (1993). Use of rhizobia in the control of root rot disease of sunflower, okra, soybean and mungbean. J. Phytopathol., 138: 157-163.
    10. Ghosh, A. C. and Basu, P.S. (2002). Growth behaviour and biproduction of indole acetic acid by Rhizobium species isolated from root nodules of a leguminous tree Dalbergia lanceolaria. Ind. J. Exp. Biol., 40: 796-801.
    11. Glickman, E. and Dessaux, Y. (1995). A critical examination of the specificity of the Salkowski reagent for indole compounds produced by pathogenic bacteria. Appl. Environ. Microbiol., 61: 793-796.
    12. Handelsman, J. and Stabb, E.V. (1996). Biocontrol of soil borne plant pathogens. The Plant Cell: 8: 1855-1869.
    13. Hartman, A., Singh, M. and Klingmuler, W. (1983). Isolation and characterization of Azospirillum mutants excreting high amount of indole acetic acid. Can. J. Microbiol., 29: 916-923.
    14. Howell, C.R. and Stipanovic, R.D. Suppression of Pythium ultimum induced damping off of cotton seedlings by Pseudomonas fluorescence and its antibiotic, pyoluteorin. (1980). Phytopathol. 70:712-715.
    15. Idris, H.A.,Labuschagne, N. and Korsten, L. (2007). Screening of rhizobacteria for biocontrol of Fusarium root and crown rot of Sorghum in Ethiopia. Biol. Control. 40:97-106.
    16. Jaymani, A.K. (2006). Studies on antagonistic effect of rhizobacteria against soil borne Phytophthora species in strawberry. M.Sc. Thesis. Der Universtat Hanover.
    17. Lemessa, F. and Zeller, W. (2007). Screening of rhizobacteria for biological control of Ralstonia solanacearum in Ethiopia. Biol. Control., 42: 336-344.
    18. Neilands, J.B. and Leong, S.A. (1986). Siderophores in relation to plant growth and disease. Ann. Rev. Pl. Physiol., 37:187-208.
    19. Park, M., Paul, D., Kim, Y.K., Nam, K.W., Lee, Y.K., Choi, H.W. and Lee, S.Y. (2007). Induced systemic resistance by Bacillus vallismortis EXTN-1 suppressed bacterial wilt in tomato caused by Ralstonia solanacearum. Pl. Pathol., 23: 22-25.
    20. Persmark, M., Pittman, P., Buyer, J.S., Schwyn, B., Gill, P.R. and Neiland, J.B. Isolation and structure of Rhizobactin 1021, a siderophore from the alfalfa symbiont Rhizobium meliloti 1021. (1993). J. Am. Chem. Soc., 115: 3950-3956.
    21. Pikovskaya, R.I. (1948). Mobilization of phosphorus in soil in connection with the vital activity of some microbial species. Microbiologica., 17: 362-370.
    22. Romero, D., de Viucente, A., Rakotoaly, R.H., Dufour, S.E., Veening, J.W., Arrebola, E., Cazorla, F.N., Quipers, O.P., Paquot, M. and Perez-Garcia, A. (2007). The Iturin and Fengycin families of lipopeptides are key factors in antagonisms of Bacillus subtilis towards Podoshaera fusca. Mol. Pl. Microbe Interact., 20: 430-440.
    23. Schwyn, B. and Neilands, J.B. Universal chemical assay for detection and determination of siderophore. (1987). Anal. Biochem., 160: 47-56.
    24. Sessitsch, A., Reiter, B. and Berg, G. (2004). Endophytic bacterial communities of field grown potato plants and their plant growth promoting and antagonistic abilities. Can. J. Microbiol., 50: 239-249.
    25. Sindhu, S.S., Gupta, S.K. and Dadarwal, K.R. (1999). Antagonistic effect of Pseudomonas spp. on pathogenic fungi and enhanced plant growth in green gram (Vigna radiata). Biol. Fertil. Soil., 29: 62-68.
    26. Sridevi, N. and Mallaiah, K.V. (2008). Production of indole-3-acetic acid by Rhizobium isolates from Sesbania species. Pl. Sci. Res., 1: 13-16.
    27. Vincent, J.M. (1970). A Manual for the Practical Study of Root Nodule Bacteria. Blackwell Scientific Publications, Oxford, England.
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